Restriction map for
pFN21AB3331
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstUI Bsu36I Cac8I CelII
CfoI Cfr10I CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HphI KasI KpnI KspI
MaeII MluI MluNI MroI MscI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1462 GTATAC GT^[AC][GT]AC
AflIII 68 ACATGT A^C[GA][CT]GT
AlwNI 305 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 771 GAGCCC G[GA]GC[CT]^C
BbsI 796 GAAGAC GAAGAC(2/6)
BclI 1410 TGATCA T^GATCA
BglII 686 AGATCT A^GATCT
BmyI 771 GAGCCC G[AGT]GC[ACT]^C
BpuAI 796 GAAGAC GAAGAC(2/6)
BsaBI 409 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1228 TCCGGT [AT]^CCGG[AT]
BseRI 7 GAGGAG GAGGAG(10/8)
BsiYI 304 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 304 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 502 GAGAC GTCTC(1/5)
Bsp1286I 771 GAGCCC G[AGT]GC[ACT]^C
BspDI 898 ATCGAT AT^CGAT
BspLU11I 68 ACATGT A^CATGT
BspMI 283 GCAGGT ACCTGC(4/8)
Bst1107I 1462 GTATAC GTA^TAC
BstEII 909 GGTAACC G^GT[ACGT]ACC
BstPI 909 GGTAACC G^GT[ACGT]ACC
BstXI 1501 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1462 GTATAC GTA^TAC
ClaI 898 ATCGAT AT^CGAT
DsaI 577 CCGTGG C^C[GA][CT]GG
EcoO65I 909 GGTAACC G^GT[ACGT]ACC
EcoRI 486 GAATTC G^AATTC
EcoT14I 401 CCAAGG C^C[AT][AT]GG
EcoT22I 1306 ATGCAT ATGCA^T
FauI 747 GCGGG CCCGC(4/6)
FbaI 1410 TGATCA T^GATCA
HapII 1229 CCGG C^CGG
HindIII 139 AAGCTT A^AGCTT
HpaII 1229 CCGG C^CGG
MamI 409 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 491 CAATTG C^AATTG
MspI 1229 CCGG C^CGG
MunI 491 CAATTG C^AATTG
NsiI 1306 ATGCAT ATGCA^T
NspI 68 ACATGT [GA]CATG^[CT]
PflMI 304 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 114 GAGTC GAGTC(4/5)
PstI 1019 CTGCAG CTGCA^G
ScaI 739 AGTACT AGT^ACT
SspI 1392 AATATT AAT^ATT
StyI 401 CCAAGG C^C[AT][AT]GG
Van91I 304 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 556 CCAAAACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 379 GCGG CCGC(-3/-1)
747 GCGG
BanI 569 GGCACC G^G[CT][GA]CC
1300 GGCACC
BfaI 428 CTAG C^TAG
701 CTAG
BsmFI 692 GGGAC GGGAC(10/14)
1316 GGGAC
BsmI 213 GAATGC GAATGC(1/-1)
347 GAATGC
Bsp1407I 27 TGTACA T^GTACA
966 TGTACA
BsrGI 27 TGTACA T^GTACA
966 TGTACA
EarI 54 GAAGAG CTCTTC(1/4)
109 GAAGAG
Ksp632I 54 GAAGAG CTCTTC(1/4)
109 GAAGAG
MaeI 428 CTAG C^TAG
701 CTAG
NdeI 169 CATATG CA^TATG
227 CATATG
SspBI 27 TGTACA T^GTACA
966 TGTACA
Tsp45I 116 GTCAC ^GT[GC]AC
1096 GTGAC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BpmI BsaJI BsgI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HinfI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII