(Circular) (Six-base) MAPSORT of: pFN21AB3331.seq Check: 2381 from: 1 to: 6247 pFN21AB3331 6247 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3468 3590 1937 Size: 1531 122 4594 Fragments arranged by size: 4594 1531 122 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3465 4310 5694 5962 1632 Size: 1833 845 1384 268 1917 Fragments arranged by size: 1917 1833 1384 845 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 2073 5146 6123 1203 Size: 870 3073 977 1327 Fragments arranged by size: 3073 1327 977 870 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 2042 1166 Size: 876 5371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6247 AlwNI CAG_nnn'CTG Cuts at: 2315 3990 5562 2315 Size: 1675 1572 3000 Fragments arranged by size: 3000 1675 1572 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 2491 3200 3268 3556 3782 3934 6043 1493 Size: 998 709 68 288 226 152 2109 1697 Fragments arranged by size: 2109 1697 998 709 288 226 152 68 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 3572 1870 Size: 73 1629 4545 Fragments arranged by size: 4545 1629 73 BamHI G'GATC_C Cuts at: 3577 3944 3577 Size: 367 5880 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2574 3305 3568 5023 618 Size: 325 217 627 787 731 263 1455 1842 Fragments arranged by size: 1842 1455 787 731 627 325 263 217 BanII G_rGCy'C Cuts at: 1391 2780 3566 1391 Size: 1389 786 4072 Fragments arranged by size: 4072 1389 786 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2808 928 Size: 971 909 4367 Fragments arranged by size: 4367 971 909 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4438 4472 1903 Size: 34 2501 34 3678 Fragments arranged by size: 3678 2501 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4139 5349 4139 Size: 1210 5037 BclI T'GATC_A Cuts at: 1548 3415 1548 Size: 1867 4380 BfrBI ATG'CAT Cuts at: 3313 3313 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4858 136 Size: 107 122 71 631 605 3186 1525 Fragments arranged by size: 3186 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2691 6242 2691 Size: 3551 2696 BlpI GC'TnA_GC Cuts at: 3664 3664 Size: 6247 Bme1580I G_kGCm'C Cuts at: 1790 4308 5464 1790 Size: 2518 1156 2573 Fragments arranged by size: 2573 2518 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6247 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 2742 3978 Size: 416 53 83 399 24 1146 179 1236 Cuts at: 3978 4936 442 Size: 958 1753 Fragments arranged by size: 1753 1236 1146 958 416 399 179 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3162 3312 3346 4907 1239 Size: 1923 150 34 1561 2579 Fragments arranged by size: 2579 1923 1561 150 34 Bpu10I CC'TnA_GC Cuts at: 2380 2380 Size: 6247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4910 882 Size: 617 3411 2219 Fragments arranged by size: 3411 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4038 493 Size: 711 2834 2702 Fragments arranged by size: 2834 2702 711 BsaBI GATnn'nnATC Cuts at: 2418 6241 2418 Size: 3823 2424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4054 4436 5071 275 Size: 53 83 186 936 2521 382 635 1451 Fragments arranged by size: 2521 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3233 4680 5078 5352 5499 5910 3233 Size: 1447 398 274 147 411 3570 Fragments arranged by size: 3570 1447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3270 3300 1178 Size: 30 165 30 1867 30 4125 Fragments arranged by size: 4125 1867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 1186 Size: 841 5406 BseYI C'CCAG_C Cuts at: 2619 2820 5450 5946 2619 Size: 201 2630 496 2920 Fragments arranged by size: 2920 2630 496 201 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2355 2514 2892 2922 1163 Size: 573 619 159 378 30 4488 Fragments arranged by size: 4488 619 573 378 159 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4308 4393 5464 4308 Size: 85 1071 5091 Fragments arranged by size: 5091 1071 85 BsmI GAATG_Cn' Cuts at: 2224 2358 3758 3851 2224 Size: 134 1400 93 4620 Fragments arranged by size: 4620 1400 134 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2780 3566 4308 4393 5464 1391 Size: 399 990 786 742 85 1071 2174 Fragments arranged by size: 2174 1071 990 786 742 399 85 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 4136 5866 4136 Size: 1730 4517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2279 3602 844 Size: 1435 1323 3489 Fragments arranged by size: 3489 1435 1323 BsrBI CCG'CTC Cuts at: 1394 1823 4134 1394 Size: 429 2311 3507 Fragments arranged by size: 3507 2311 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4742 4916 65 Size: 1183 3494 174 1396 Fragments arranged by size: 3494 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4891 5078 1700 Size: 110 3081 187 2869 Fragments arranged by size: 3081 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2032 2971 96 Size: 1936 939 3372 Fragments arranged by size: 3372 1936 939 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6247 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 2914 1184 Size: 1730 4517 BstXI CCAn_nnnn'nTGG Cuts at: 3513 3513 Size: 6247 BstYI r'GATC_y Cuts at: 1972 2343 2669 2691 2824 3076 3577 3944 4329 Size: 371 326 22 133 252 501 367 385 Cuts at: 4329 4346 5798 5884 5896 6242 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 501 385 371 367 346 326 252 133 86 22 17 12 BstZ17I GTA'TAC Cuts at: 3469 3469 Size: 6247 BtgI C'CryG_G Cuts at: 513 1065 2004 2582 513 Size: 552 939 578 4178 Fragments arranged by size: 4178 939 578 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2501 487 Size: 683 56 1275 4233 Fragments arranged by size: 4233 1275 683 56 BtsI GCAGTG_nn' Cuts at: 2880 3200 3763 4557 4577 2880 Size: 320 563 794 20 4550 Fragments arranged by size: 4550 794 563 320 20 ClaI AT'CG_AT Cuts at: 1554 2904 1554 Size: 1350 4897 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5254 809 Size: 507 3938 1802 Fragments arranged by size: 3938 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212 Size: 54 1425 50 275 18 1864 889 1629 Cuts at: 6212 8 Size: 43 Fragments arranged by size: 1864 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2109 4177 1353 Size: 701 55 2068 3423 Fragments arranged by size: 3423 2068 701 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4834 5208 5354 387 Size: 1064 138 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3162 3312 3346 3465 4310 4907 5694 Size: 393 1530 150 34 119 845 597 787 Cuts at: 5694 5962 1239 Size: 268 1524 Fragments arranged by size: 1530 1524 845 787 597 393 268 150 119 34 EcoO109I rG'GnC_Cy Cuts at: 2064 2210 2312 2064 Size: 146 102 5999 Fragments arranged by size: 5999 146 102 EcoRI G'AATT_C Cuts at: 2491 3556 3934 2491 Size: 1065 378 4804 Fragments arranged by size: 4804 1065 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2213 2213 Size: 6247 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 5394 1678 Size: 3716 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2767 2799 3374 3406 4888 Size: 32 135 32 1195 32 575 32 1482 Cuts at: 4888 4920 4962 4994 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1482 1195 575 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3591 3837 669 Size: 988 281 1653 246 3079 Fragments arranged by size: 3079 1653 988 281 246 HindIII A'AGCT_T Cuts at: 748 2144 748 Size: 1396 4851 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 2496 3846 2496 Size: 1350 4897 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5361 5545 1305 Size: 4056 184 2007 Fragments arranged by size: 4056 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4425 Fragments arranged by size: 4425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2856 2868 2994 4205 4564 4723 Size: 648 876 814 12 126 1211 359 159 Cuts at: 4723 518 Size: 2042 Fragments arranged by size: 2042 1211 876 814 648 359 159 126 12 MspA1I CmG'CkG Cuts at: 1606 2369 3362 3443 3513 3663 4340 5488 5733 Size: 763 993 81 70 150 677 1148 245 Cuts at: 5733 1606 Size: 2120 Fragments arranged by size: 2120 1148 993 763 677 245 150 81 70 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4756 Fragments arranged by size: 4756 939 552 NdeI CA'TA_TG Cuts at: 387 2175 2233 387 Size: 1788 58 4401 Fragments arranged by size: 4401 1788 58 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NsiI A_TGCA'T Cuts at: 3315 3315 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 2077 3605 5150 1132 Size: 97 848 1528 1545 2229 Fragments arranged by size: 2229 1545 1528 848 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6247 PciI A'CATG_T Cuts at: 2073 5146 2073 Size: 3073 3174 PflMI CCAn_nnn'nTGG Cuts at: 1108 2315 1108 Size: 1207 5040 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 5829 5861 4316 Size: 32 1481 32 4702 Fragments arranged by size: 4702 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2064 2210 2312 2064 Size: 146 102 5999 Fragments arranged by size: 5999 146 102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PstI C_TGCA'G Cuts at: 830 3028 3599 830 Size: 2198 571 3478 Fragments arranged by size: 3478 2198 571 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 PvuII CAG'CTG Cuts at: 2369 3362 3443 3513 2369 Size: 993 81 70 5103 Fragments arranged by size: 5103 993 81 70 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 2746 4495 6207 2746 Size: 1749 1712 2786 Fragments arranged by size: 2786 1749 1712 SfcI C'TryA_G Cuts at: 826 1045 2481 2593 2920 3006 3024 3595 4730 Size: 219 1436 112 327 86 18 571 1135 Cuts at: 4730 5411 5602 826 Size: 681 191 1471 Fragments arranged by size: 1471 1436 1135 681 571 327 219 191 112 86 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4351 5252 5514 820 Size: 197 926 2408 901 262 1553 Fragments arranged by size: 2408 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 3399 4171 5 Size: 47 3347 772 2081 Fragments arranged by size: 3347 2081 772 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6247 StyI C'CwwG_G Cuts at: 513 1065 2004 2406 3686 513 Size: 552 939 402 1280 3074 Fragments arranged by size: 3074 1280 939 552 402 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2592 4274 4291 4444 4629 5074 325 Size: 1255 1012 1682 17 153 185 445 1498 Fragments arranged by size: 1682 1498 1255 1012 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2032 2744 2971 Size: 274 80 33 51 1443 55 712 227 Cuts at: 2971 4493 6205 96 Size: 1522 1712 138 Fragments arranged by size: 1712 1522 1443 712 274 227 138 80 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 6207 6239 2085 Size: 32 4090 32 2093 Fragments arranged by size: 4090 2093 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2568 2568 Size: 6247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 4376 4376 Size: 6247 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI