Restriction map for
pFN21AB3313
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# NO CUT ENZYMES ( 105 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspHI AvaI
AviII BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BsaWI BseAI BsgI BsiHKAI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI Eco47III EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 294 GACGTC GACGT^C
AcsI 133 AAATTT [GA]^AATT[CT]
AcyI 294 GACGTC G[GA]^CG[CT]C
AflIII 1076 ACATGT A^C[GA][CT]GT
AhdI 289 GACCAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 267 CCTCAGG CC^T[ACGT]AGG
ApoI 133 AAATTT [GA]^AATT[CT]
Asp700I 117 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 289 GACCAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1096 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1278 CCTAGG C^CTAGG
BlnI 1278 CCTAGG C^CTAGG
BpmI 1211 CTGGAG CTGGAG(16/14)
BsaAI 1069 TACGTG [CT]AC^GT[GA]
BsaHI 294 GACGTC G[GA]^CG[CT]C
BsaI 826 GGTCTC GGTCTC(1/5)
BseRI 343 GAGGAG GAGGAG(10/8)
BsiEI 48 CGGCCG CG[GA][CT]^CG
Bsp1407I 1589 TGTACA T^GTACA
BspLU11I 1076 ACATGT A^CATGT
BspMI 967 ACCTGC ACCTGC(4/8)
BsrGI 1589 TGTACA T^GTACA
BstAPI 1238 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 267 CCTCAGG CC^T[ACGT]AGG
CpoI 582 CGGACCG CG^G[AT]CCG
DraI 598 TTTAAA TTT^AAA
DraIII 871 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1380 GACAGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1565 CCATGG C^C[GA][CT]GG
EagI 48 CGGCCG C^GGCCG
Eam1105I 289 GACCAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 48 CGGCCG C^GGCCG
Eco52I 48 CGGCCG C^GGCCG
Eco81I 267 CCTCAGG CC^T[ACGT]AGG
EcoRV 53 GATATC GAT^ATC
HgaI 1090 GACGC GACGC(5/10)
Hin1I 294 GACGTC G[GA]^CG[CT]C
MslI 768 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1565 CCATGG C^CATGG
NspI 1076 ACATGT [GA]CATG^[CT]
PflFI 1096 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1251 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1013 AGGACCT [GA]G^G[AT]CC[CT]
RsrII 582 CGGACCG CG^G[AT]CCG
SapI 449 GCTCTTC GCTCTTC(1/4)
SexAI 1016 ACCTGGT A^CC[AT]GGT
SspBI 1589 TGTACA T^GTACA
Tth111I 1096 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1251 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1433 CCAGCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 117 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 109 CGCG CG^CG
594 CGCG
ApaI 929 GGGCCC GGGCC^C
1026 GGGCCC
BamHI 996 GGATCC G^GATCC
1049 GGATCC
BbsI 1263 GAAGAC GAAGAC(2/6)
441 GTCTTC
BcnI 503 CCCGG CC^[GC]GG
549 CCCGG
BfaI 557 CTAG C^TAG
1279 CTAG
BlpI 663 GCTAAGC GC^T[ACGT]AGC
1523 GCTTAGC
Bpu1102I 663 GCTAAGC GC^T[ACGT]AGC
1523 GCTTAGC
BpuAI 1263 GAAGAC GAAGAC(2/6)
441 GTCTTC
BsaBI 634 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1308 GATGCTGATC
BsrDI 1530 CATTGC GCAATG(2/0)
1292 GCAATG
BstUI 109 CGCG CG^CG
594 CGCG
CelII 663 GCTAAGC GC^T[ACGT]AGC
1523 GCTTAGC
FauI 949 CCCGC CCCGC(4/6)
510 GCGGG
HaeII 1139 GGCGCT [GA]GCGC^[CT]
1605 GGCGCT
HincII 1080 GTCAAC GT[CT]^[GA]AC
1549 GTCAAC
HindII 1080 GTCAAC GT[CT]^[GA]AC
1549 GTCAAC
MaeI 557 CTAG C^TAG
1279 CTAG
MamI 634 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1308 GATGCTGATC
MvnI 109 CGCG CG^CG
594 CGCG
NciI 503 CCCGG CC^[GC]GG
549 CCCGG
TaqI 257 TCGA T^CGA
327 TCGA
TthHB8I 257 TCGA T^CGA
327 TCGA
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanII BbvI BmyI
BsaJI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII