(Circular) (Six-base) MAPSORT of: pFN21AB3313.seq Check: 8280 from: 1 to: 6364 pFN21AB3313 6364 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2303 4174 278 Size: 53 83 186 936 767 1871 2468 Fragments arranged by size: 2468 1871 936 767 186 83 53 AccI GT'mk_AC Cuts at: 1937 3707 1937 Size: 1770 4594 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2204 2421 2433 2442 2979 4427 5811 6079 Size: 572 217 12 9 537 1448 1384 268 Cuts at: 6079 1632 Size: 1917 Fragments arranged by size: 1917 1448 1384 572 537 268 217 12 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 3081 3661 5263 6240 1203 Size: 1878 580 1602 977 1327 Fragments arranged by size: 1878 1602 1327 977 580 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 2299 5093 2299 Size: 2794 3570 AleI CACnn'nnGTG Cuts at: 1166 2777 1166 Size: 1611 4753 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6364 AlwNI CAG_nnn'CTG Cuts at: 2885 3181 3249 4107 5679 2885 Size: 296 68 858 1572 3570 Fragments arranged by size: 3570 1572 858 296 68 ApaI G_GGCC'C Cuts at: 2938 3035 2938 Size: 97 6267 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 2138 3673 3899 4051 6160 1493 Size: 645 1535 226 152 2109 1697 Fragments arranged by size: 2109 1697 1535 645 226 152 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 3689 1870 Size: 73 1746 4545 Fragments arranged by size: 4545 1746 73 AvrII C'CTAG_G Cuts at: 3283 3283 Size: 6364 BamHI G'GATC_C Cuts at: 3001 3054 3694 4061 3001 Size: 53 640 367 5304 Fragments arranged by size: 5304 640 367 53 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3685 5140 618 Size: 325 217 627 1898 1455 1842 Fragments arranged by size: 1898 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2494 2938 3035 3683 1391 Size: 1103 444 97 648 4072 Fragments arranged by size: 4072 1103 648 444 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2439 3275 928 Size: 971 540 836 4017 Fragments arranged by size: 4017 971 836 540 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4555 4589 1903 Size: 34 2618 34 3678 Fragments arranged by size: 3678 2618 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4256 5466 4256 Size: 1210 5154 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4975 136 Size: 107 122 71 631 605 337 2966 1525 Fragments arranged by size: 2966 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 2669 3529 3781 2669 Size: 860 252 5252 Fragments arranged by size: 5252 860 252 Bme1580I G_kGCm'C Cuts at: 1790 2938 3035 4425 5581 1790 Size: 1148 97 1390 1156 2573 Fragments arranged by size: 2573 1390 1156 1148 97 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6364 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3030 4095 5053 Size: 416 53 83 399 24 1613 1065 958 Cuts at: 5053 442 Size: 1753 Fragments arranged by size: 1753 1613 1065 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3237 5024 1239 Size: 1998 1787 2579 Fragments arranged by size: 2579 1998 1787 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4489 5354 5652 4489 Size: 865 298 5201 Fragments arranged by size: 5201 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2837 5027 882 Size: 617 1338 2190 2219 Fragments arranged by size: 2219 2190 1338 617 BsaAI yAC'GTr Cuts at: 493 1204 3076 4155 493 Size: 711 1872 1079 2702 Fragments arranged by size: 2702 1872 1079 711 BsaBI GATnn'nnATC Cuts at: 2643 3317 6358 2643 Size: 674 3041 2649 Fragments arranged by size: 3041 2649 674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2300 4171 4553 5188 Size: 53 83 186 936 767 1871 382 635 Cuts at: 5188 275 Size: 1451 Fragments arranged by size: 1871 1451 936 767 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4797 5195 5469 5616 6027 4797 Size: 398 274 147 411 5134 Fragments arranged by size: 5134 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6139 Fragments arranged by size: 6139 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 1186 Size: 1177 5187 BseYI C'CCAG_C Cuts at: 3028 3396 3541 5567 6063 3028 Size: 368 145 2026 496 3329 Fragments arranged by size: 3329 2026 496 368 145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5791 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2056 3814 4575 5603 1540 Size: 275 186 55 1758 761 1028 2301 Fragments arranged by size: 2301 1758 1028 761 275 186 55 BsiHKAI G_wGCw'C Cuts at: 4425 4510 5581 4425 Size: 85 1071 5208 Fragments arranged by size: 5208 1071 85 BsmI GAATG_Cn' Cuts at: 3875 3968 3875 Size: 93 6271 BsmBI CGTCTCn'nnnn_ Cuts at: 2857 2971 3169 2857 Size: 114 198 6052 Fragments arranged by size: 6052 198 114 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2494 2938 3035 3683 4425 4510 5581 Size: 399 704 444 97 648 742 85 1071 Cuts at: 5581 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 704 648 444 399 97 85 BspEI T'CCGG_A Cuts at: 6027 6027 Size: 6364 BspHI T'CATG_A Cuts at: 4253 5983 4253 Size: 1730 4634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2981 3719 844 Size: 2137 738 3489 Fragments arranged by size: 3489 2137 738 BsrBI CCG'CTC Cuts at: 1394 1823 4251 1394 Size: 429 2428 3507 Fragments arranged by size: 3507 2428 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3304 3534 4859 5033 65 Size: 1183 2056 230 1325 174 1396 Fragments arranged by size: 2056 1396 1325 1183 230 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5008 5195 1700 Size: 110 3198 187 2869 Fragments arranged by size: 3198 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3594 96 Size: 3498 2866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6364 BssSI C'ACGA_G Cuts at: 4424 5436 4424 Size: 1012 5352 BstAPI GCAn_nnn'nTGC Cuts at: 794 3249 794 Size: 2455 3909 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstXI CCAn_nnnn'nTGG Cuts at: 3022 3541 3613 3022 Size: 519 72 5773 Fragments arranged by size: 5773 519 72 BstYI r'GATC_y Cuts at: 1972 2542 2644 3001 3054 3520 3694 4061 4446 Size: 570 102 357 53 466 174 367 385 Cuts at: 4446 4463 5915 6001 6013 6359 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 570 466 385 367 357 346 174 102 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2273 2273 Size: 6364 BtgI C'CryG_G Cuts at: 513 1065 2004 3570 513 Size: 552 939 1566 3307 Fragments arranged by size: 3307 1566 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3275 487 Size: 683 56 2049 3576 Fragments arranged by size: 3576 2049 683 56 BtsI GCAGTG_nn' Cuts at: 2898 3880 4674 4694 2898 Size: 982 794 20 4568 Fragments arranged by size: 4568 982 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 2605 3668 2605 Size: 1063 5301 DraIII CAC_nnn'GTG Cuts at: 2881 2881 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3391 5371 809 Size: 507 2075 1980 1802 Fragments arranged by size: 2075 1980 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2069 2583 Size: 54 1425 50 275 18 223 16 514 Cuts at: 2583 2595 3012 3348 3435 3811 4700 6329 8 Size: 12 417 336 87 376 889 1629 43 Fragments arranged by size: 1629 1425 889 514 417 376 336 275 223 87 54 50 43 18 16 12 EagI C'GGCC_G Cuts at: 1537 1812 2053 3811 1537 Size: 275 241 1758 4090 Fragments arranged by size: 4090 1758 275 241 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 2432 2461 2727 4294 1353 Size: 906 173 29 266 1567 3423 Fragments arranged by size: 3423 1567 906 266 173 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3047 3628 4951 5325 5471 387 Size: 1064 138 1458 581 1323 374 146 1280 Fragments arranged by size: 1458 1323 1280 1064 581 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2421 2433 2442 2979 3237 4427 Size: 393 572 217 12 9 537 258 1190 Cuts at: 4427 5024 5811 6079 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1190 787 597 572 537 393 268 258 217 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 2070 3377 3422 2070 Size: 1307 45 5012 Fragments arranged by size: 5012 1307 45 EcoO109I rG'GnC_Cy Cuts at: 2934 3019 3032 2934 Size: 85 13 6266 Fragments arranged by size: 6266 85 13 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 EcoRV GAT'ATC Cuts at: 2060 2060 Size: 6364 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2143 3292 2143 Size: 1149 5215 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 3148 3614 5511 1678 Size: 1470 466 1897 2531 Fragments arranged by size: 2531 1897 1470 466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2071 2103 2285 2317 5005 Size: 32 135 32 499 32 182 32 2688 Cuts at: 5005 5037 5079 5111 1373 Size: 32 42 32 2626 Fragments arranged by size: 2688 2626 499 182 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3556 3708 3954 669 Size: 988 281 1149 469 152 246 3079 Fragments arranged by size: 3079 1149 988 469 281 246 152 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2880 2979 3587 5478 5662 1305 Size: 1575 99 608 1891 184 2007 Fragments arranged by size: 2007 1891 1575 608 184 99 MscI TGG'CCA Cuts at: 10 64 1489 1832 2071 3350 3437 10 Size: 54 1425 343 239 1279 87 2937 Fragments arranged by size: 2937 1425 1279 343 239 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 4322 4681 4840 518 Size: 648 1611 1545 359 159 2042 Fragments arranged by size: 2042 1611 1545 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2690 2765 2957 3181 3249 3578 3780 Size: 576 508 75 192 224 68 329 202 Cuts at: 3780 4457 5605 5850 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 576 508 329 245 224 202 192 75 68 NcoI C'CATG_G Cuts at: 513 1065 2004 3570 513 Size: 552 939 1566 3307 Fragments arranged by size: 3307 1566 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 3085 3722 5267 1132 Size: 97 1856 637 1545 2229 Fragments arranged by size: 2229 1856 1545 637 97 PasI CC'CwG_GG Cuts at: 1348 3062 1348 Size: 1714 4650 PciI A'CATG_T Cuts at: 3081 5263 3081 Size: 2182 4182 PflMI CCAn_nnn'nTGG Cuts at: 1108 3262 1108 Size: 2154 4210 PfoI T'CCnGG_A Cuts at: 2553 2925 3447 2553 Size: 372 522 5470 Fragments arranged by size: 5470 522 372 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3019 3019 Size: 6364 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2934 3031 2934 Size: 97 6267 PstI C_TGCA'G Cuts at: 830 2905 3245 3583 3716 830 Size: 2075 340 338 133 3478 Fragments arranged by size: 3478 2075 340 338 133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 PvuII CAG'CTG Cuts at: 2182 2690 2765 3181 3249 3578 2182 Size: 508 75 416 68 329 4968 Fragments arranged by size: 4968 508 416 329 75 68 RsrII CG'GwC_CG Cuts at: 2588 2588 Size: 6364 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SapI GCTCTTCn'nnn_ Cuts at: 2461 2461 Size: 6364 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SexAI A'CCwGG_T Cuts at: 3021 3021 Size: 6364 SfcI C'TryA_G Cuts at: 826 1045 2901 3241 3579 3712 4847 5528 5719 Size: 219 1856 340 338 133 1135 681 191 Cuts at: 5719 826 Size: 1471 Fragments arranged by size: 1856 1471 1135 681 340 338 219 191 133 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3691 1872 Size: 1819 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4468 5369 5631 820 Size: 197 926 2525 901 262 1553 Fragments arranged by size: 2525 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3722 1132 Size: 2590 3774 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6364 StyI C'CwwG_G Cuts at: 513 1065 2004 2066 2827 3006 3015 3283 3334 Size: 552 939 62 761 179 9 268 51 Cuts at: 3334 3570 3606 3803 513 Size: 236 36 197 3074 Fragments arranged by size: 3074 939 761 552 268 236 197 179 62 51 36 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2605 2812 3533 4391 4408 4561 4746 Size: 1255 1025 207 721 858 17 153 185 Cuts at: 4746 5191 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1025 858 721 445 207 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3594 4610 6322 Size: 274 80 33 51 1443 1617 1016 1712 Cuts at: 6322 96 Size: 138 Fragments arranged by size: 1712 1617 1443 1016 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3443 3475 6324 6356 3443 Size: 32 2849 32 3451 Fragments arranged by size: 3451 2849 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3104 1316 Size: 1788 4576 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3445 3445 Size: 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 3689 1870 Size: 1819 4545 XmnI GAAnn'nnTTC Cuts at: 2126 4493 2126 Size: 2367 3997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2301 4172 276 Size: 53 83 186 936 767 1871 2468 Fragments arranged by size: 2468 1871 936 767 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SfiI SfoI SrfI SwaI