Restriction map for
pFN21AB3292
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspEI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1238 GGTACC G^GTACC
AclI 554 AACGTT AA^CGTT
AseI 418 ATTAAT AT^TAAT
AsnI 418 ATTAAT AT^TAAT
Asp718I 1238 GGTACC G^GTACC
AspHI 387 GAGCAC G[AT]GC[AT]^C
BalI 668 TGGCCA TGG^CCA
BglII 286 AGATCT A^GATCT
BseRI 950 GAGGAG GAGGAG(10/8)
BsiHKAI 387 GAGCAC G[AT]GC[AT]^C
BsmI 88 GAATGC GAATGC(1/-1)
Bsp1407I 640 TGTACA T^GTACA
BspDI 712 ATCGAT AT^CGAT
BspHI 162 TCATGA T^CATGA
BsrDI 1619 GCAATG GCAATG(2/0)
BsrGI 640 TGTACA T^GTACA
BstYI 286 AGATCT [GA]^GATC[CT]
Cac8I 748 GCAGGC GC[ACGT]^[ACGT]GC
ClaI 712 ATCGAT AT^CGAT
DdeI 1204 CTAAG C^T[ACGT]AG
DraI 235 TTTAAA TTT^AAA
DraII 168 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 668 TGGCCA [CT]^GGCC[GA]
EcoNI 160 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 168 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1126 GAATTC G^AATTC
KpnI 1238 GGTACC GGTAC^C
MflI 286 AGATCT [GA]^GATC[CT]
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MslI 711 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 923 CATATG CA^TATG
PflMI 1377 CCATGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 846 GACTC GAGTC(4/5)
PpuMI 168 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 418 ATTAAT AT^TAAT
Psp1406I 554 AACGTT AA^CGTT
PstI 1543 CTGCAG CTGCA^G
RcaI 162 TCATGA T^CATGA
SfcI 1543 CTGCAG C^T[GA][CT]AG
SspBI 640 TGTACA T^GTACA
SspI 1671 AATATT AAT^ATT
Van91I 1377 CCATGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 286 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 210 GGATC GGATC(4/5)
674 GGATC
AlwNI 749 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1564 CAGTGCCTG
Asp700I 448 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1134 GAATTTCTTC
BanI 1238 GGTACC G^G[CT][GA]CC
1599 GGTGCC
BciVI 330 GTATCC GTATCC(6/5)
801 GTATCC
BmyI 387 GAGCAC G[AGT]GC[ACT]^C
1600 GTGCCC
BsaJI 869 CCATGG C^C[ACGT][ACGT]GG
1377 CCATGG
BsmAI 479 GAGAC GTCTC(1/5)
1613 GAGAC
BsmFI 704 GTCCC GGGAC(10/14)
1632 GTCCC
Bsp1286I 387 GAGCAC G[AGT]GC[ACT]^C
1600 GTGCCC
BspLU11I 1020 ACATGT A^CATGT
1029 ACATGT
BstBI 542 TTCGAA TT^CGAA
1649 TTCGAA
DsaI 869 CCATGG C^C[GA][CT]GG
1377 CCATGG
EarI 848 CTCTTC CTCTTC(1/4)
1585 GAAGAG
EcoT14I 869 CCATGG C^C[AT][AT]GG
1377 CCATGG
Ksp632I 848 CTCTTC CTCTTC(1/4)
1585 GAAGAG
NcoI 869 CCATGG C^CATGG
1377 CCATGG
NspV 542 TTCGAA TT^CGAA
1649 TTCGAA
SfuI 542 TTCGAA TT^CGAA
1649 TTCGAA
StuI 493 AGGCCT AGG^CCT
879 AGGCCT
StyI 869 CCATGG C^C[AT][AT]GG
1377 CCATGG
TfiI 1261 GATTC G^A[AT]TC
1317 GAATC
Tsp45I 1277 GTCAC ^GT[GC]AC
1657 GTCAC
XbaI 141 TCTAGA T^CTAGA
1326 TCTAGA
XmnI 448 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1134 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
AvaII BbvI BclI BfaI BpmI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cfr13I DpnII EcoRII FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I