(Circular) (Six-base) MAPSORT of: pFN21AB3292.seq Check: 9345 from: 1 to: 6382 pFN21AB3292 6382 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3725 1937 Size: 1788 4594 Acc65I G'GTAC_C Cuts at: 3243 3703 3243 Size: 460 5922 AclI AA'CG_TT Cuts at: 1563 2560 4509 4882 1563 Size: 997 1949 373 3063 Fragments arranged by size: 3063 1949 997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2071 4445 5829 6097 1632 Size: 439 2374 1384 268 1917 Fragments arranged by size: 2374 1917 1384 439 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6185 AflIII A'CryG_T Cuts at: 1203 3025 3034 3060 5281 6258 1203 Size: 1822 9 26 2221 977 1327 Fragments arranged by size: 2221 1822 1327 977 26 9 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 5111 5111 Size: 6382 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2417 1184 Size: 1233 5149 AlwNI CAG_nnn'CTG Cuts at: 2759 3574 4125 5697 2759 Size: 815 551 1572 3444 Fragments arranged by size: 3444 1572 815 551 ApaLI G'TGCA_C Cuts at: 4439 5595 4439 Size: 1156 5226 ApoI r'AATT_y Cuts at: 1493 2012 2970 3131 3139 3273 3369 3691 3917 Size: 519 958 161 8 134 96 322 226 Cuts at: 3917 4069 6178 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 958 519 322 226 161 152 134 96 8 AseI AT'TA_AT Cuts at: 160 1032 2424 4936 160 Size: 872 1392 2512 1606 Fragments arranged by size: 2512 1606 1392 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 3707 1870 Size: 73 1764 4545 Fragments arranged by size: 4545 1764 73 BamHI G'GATC_C Cuts at: 3712 4079 3712 Size: 367 6015 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3243 3604 3703 5158 618 Size: 325 217 627 1456 361 99 1455 1842 Fragments arranged by size: 1842 1456 1455 627 361 325 217 99 BanII G_rGCy'C Cuts at: 1391 3701 1391 Size: 2310 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4573 4607 1903 Size: 34 2636 34 3678 Fragments arranged by size: 3678 2636 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2346 2817 4274 5484 2346 Size: 471 1457 1210 3244 Fragments arranged by size: 3244 1457 1210 471 BclI T'GATC_A Cuts at: 1548 2571 2616 2724 1548 Size: 1023 45 108 5206 Fragments arranged by size: 5206 1023 108 45 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4993 136 Size: 107 122 71 631 605 3321 1525 Fragments arranged by size: 3321 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2291 6377 2291 Size: 4086 2296 BlpI GC'TnA_GC Cuts at: 3799 3799 Size: 6382 Bme1580I G_kGCm'C Cuts at: 1790 3609 4443 5599 1790 Size: 1819 834 1156 2573 Fragments arranged by size: 2573 1819 1156 834 BmgBI CAC'GTC Cuts at: 1318 3281 1318 Size: 1963 4419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3470 3527 4113 Size: 416 53 83 399 24 2053 57 586 Cuts at: 4113 5071 442 Size: 958 1753 Fragments arranged by size: 2053 1753 958 586 416 399 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2199 2681 3144 5042 1239 Size: 918 42 482 463 1898 2579 Fragments arranged by size: 2579 1898 918 482 463 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4507 5372 5670 4507 Size: 865 298 5219 Fragments arranged by size: 5219 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5045 882 Size: 617 3546 2219 Fragments arranged by size: 3546 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4173 493 Size: 711 2969 2702 Fragments arranged by size: 2969 2702 711 BsaBI GATnn'nnATC Cuts at: 6376 6376 Size: 6382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4189 4571 5206 275 Size: 53 83 186 936 2656 382 635 1451 Fragments arranged by size: 2656 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4815 5213 5487 5634 6045 4815 Size: 398 274 147 411 5152 Fragments arranged by size: 5152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2583 2613 1178 Size: 30 165 30 1180 30 4947 Fragments arranged by size: 4947 1180 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2970 1186 Size: 1784 4598 BseYI C'CCAG_C Cuts at: 3608 3639 5585 6081 3608 Size: 31 1946 496 3909 Fragments arranged by size: 3909 1946 496 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5809 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3832 4593 5621 1540 Size: 275 186 1831 761 1028 2301 Fragments arranged by size: 2301 1831 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2396 4443 4528 5599 2396 Size: 2047 85 1071 3179 Fragments arranged by size: 3179 2047 1071 85 BsmI GAATG_Cn' Cuts at: 2099 3893 3986 2099 Size: 1794 93 4495 Fragments arranged by size: 4495 1794 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 3609 3701 4443 4528 5599 1391 Size: 399 606 1213 92 742 85 1071 2174 Fragments arranged by size: 2174 1213 1071 742 606 399 92 85 BspEI T'CCGG_A Cuts at: 6045 6045 Size: 6382 BspHI T'CATG_A Cuts at: 2167 4271 6001 2167 Size: 2104 1730 2548 Fragments arranged by size: 2548 2104 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3737 844 Size: 2893 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4269 1394 Size: 429 2446 3507 Fragments arranged by size: 3507 2446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3631 4877 5051 65 Size: 1183 2383 1246 174 1396 Fragments arranged by size: 2383 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5026 5213 1700 Size: 110 3216 187 2869 Fragments arranged by size: 3216 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2645 96 Size: 2549 3833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 4442 5454 4442 Size: 1012 5370 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6382 BstBI TT'CG_AA Cuts at: 2548 3655 5186 2548 Size: 1107 1531 3744 Fragments arranged by size: 3744 1531 1107 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6382 BstYI r'GATC_y Cuts at: 1972 2291 3712 4079 4464 4481 5933 6019 6031 Size: 319 1421 367 385 17 1452 86 12 Cuts at: 6031 6377 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1421 385 367 346 319 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2874 3382 513 Size: 552 1809 508 3513 Fragments arranged by size: 3513 1809 552 508 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5643 Fragments arranged by size: 5643 683 56 BtsI GCAGTG_nn' Cuts at: 2996 3650 3898 4692 4712 2996 Size: 654 248 794 20 4666 Fragments arranged by size: 4666 794 654 248 20 ClaI AT'CG_AT Cuts at: 1554 2718 1554 Size: 1164 5218 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2343 2378 529 Size: 35 1779 35 4533 Fragments arranged by size: 4533 1779 35 35 DraI TTT'AAA Cuts at: 2242 3686 2242 Size: 1444 4938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5389 809 Size: 507 4073 1802 Fragments arranged by size: 4073 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2673 3829 4718 Size: 54 1425 50 275 18 843 1156 889 Cuts at: 4718 6347 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1156 889 843 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3829 1537 Size: 275 2017 4090 Fragments arranged by size: 4090 2017 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2859 3585 4312 1353 Size: 1506 726 727 3423 Fragments arranged by size: 3423 1506 727 726 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4969 5343 5489 387 Size: 1064 138 3380 374 146 1280 Fragments arranged by size: 3380 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2157 2199 2681 3144 4445 5042 Size: 393 439 86 42 482 463 1301 597 Cuts at: 5042 5829 6097 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1301 787 597 482 463 439 393 268 86 42 EcoNI CCTnn'n_nnAGG Cuts at: 2169 2169 Size: 6382 EcoO109I rG'GnC_Cy Cuts at: 2174 2174 Size: 6382 EcoRI G'AATT_C Cuts at: 3131 3691 4069 3131 Size: 560 378 5444 Fragments arranged by size: 5444 560 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3160 3160 Size: 6382 FspI TGC'GCA Cuts at: 4888 4888 Size: 6382 HaeII r_GCGC'y Cuts at: 1678 5529 1678 Size: 3851 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3179 3211 5023 5055 5097 Size: 32 135 32 1607 32 1812 32 42 Cuts at: 5097 5129 1373 Size: 32 2626 Fragments arranged by size: 2626 1812 1607 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3726 3972 669 Size: 988 281 1788 246 3079 Fragments arranged by size: 3079 1788 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6382 HpaI GTT'AAC Cuts at: 3972 3972 Size: 6382 KpnI G_GTAC'C Cuts at: 3247 3707 3247 Size: 460 5922 MfeI C'AATT_G Cuts at: 3981 3981 Size: 6382 MluI A'CGCG_T Cuts at: 6258 6258 Size: 6382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3010 3346 5496 5680 1305 Size: 1705 336 2150 184 2007 Fragments arranged by size: 2150 2007 1705 336 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 10 Size: 54 1425 343 843 3717 Fragments arranged by size: 3717 1425 843 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 4340 4699 4858 518 Size: 648 1554 1620 359 159 2042 Fragments arranged by size: 2042 1620 1554 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3798 4475 5623 5868 1606 Size: 2192 677 1148 245 2120 Fragments arranged by size: 2192 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2874 3382 513 Size: 552 1809 508 3513 Fragments arranged by size: 3513 1809 552 508 NdeI CA'TA_TG Cuts at: 387 2929 387 Size: 2542 3840 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NspI r_CATG'y Cuts at: 1132 1229 3021 3029 3038 3557 3740 5285 1132 Size: 97 1792 8 9 519 183 1545 2229 Fragments arranged by size: 2229 1792 1545 519 183 97 9 8 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6382 PciI A'CATG_T Cuts at: 3025 3034 5281 3025 Size: 9 2247 4126 Fragments arranged by size: 4126 2247 9 PflMI CCAn_nnn'nTGG Cuts at: 1108 3388 1108 Size: 2280 4102 PfoI T'CCnGG_A Cuts at: 2176 2625 3067 2176 Size: 449 442 5491 Fragments arranged by size: 5491 449 442 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4451 4483 5964 5996 4451 Size: 32 1481 32 4837 Fragments arranged by size: 4837 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2174 2174 Size: 6382 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6382 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PstI C_TGCA'G Cuts at: 830 3552 3734 830 Size: 2722 182 3478 Fragments arranged by size: 3478 2722 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 SalI G'TCGA_C Cuts at: 1936 3724 1936 Size: 1788 4594 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 4630 6342 4630 Size: 1712 4670 SfcI C'TryA_G Cuts at: 826 1045 3548 3730 4865 5546 5737 826 Size: 219 2503 182 1135 681 191 1471 Fragments arranged by size: 2503 1471 1135 681 219 191 182 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 3709 1872 Size: 1837 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4486 5387 5649 820 Size: 197 926 2543 901 262 1553 Fragments arranged by size: 2543 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6382 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SspI AAT'ATT Cuts at: 5 52 3678 4306 5 Size: 47 3626 628 2081 Fragments arranged by size: 3626 2081 628 47 StuI AGG'CCT Cuts at: 1517 2500 2886 1517 Size: 983 386 5013 Fragments arranged by size: 5013 983 386 StyI C'CwwG_G Cuts at: 513 1065 2874 3382 3821 513 Size: 552 1809 508 439 3074 Fragments arranged by size: 3074 1809 552 508 439 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3624 4409 4426 4579 4764 5209 325 Size: 1255 2044 785 17 153 185 445 1498 Fragments arranged by size: 2044 1498 1255 785 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2155 2645 2745 Size: 274 80 33 51 1443 178 490 100 Cuts at: 2745 4628 6340 96 Size: 1883 1712 138 Fragments arranged by size: 1883 1712 1443 490 274 178 138 100 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2583 2615 6342 6374 2583 Size: 32 3727 32 2591 Fragments arranged by size: 3727 2591 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6382 XbaI T'CTAG_A Cuts at: 2146 3331 3718 2146 Size: 1185 387 4810 Fragments arranged by size: 4810 1185 387 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6382 XmaI C'CCGG_G Cuts at: 1870 3707 1870 Size: 1837 4545 XmnI GAAnn'nnTTC Cuts at: 2457 3143 4511 2457 Size: 686 1368 4328 Fragments arranged by size: 4328 1368 686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI