Restriction map for
pFN21AB3292
(Linear) (Six-base) MAPSORT of: KIBB3292.seq Check: 5634 from: 1 to: 1677
KIBB3292.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
Acc65I G'GTAC_C
Cuts at: 0 1238 1677
Size: 1238 439
AclI AA'CG_TT
Cuts at: 0 555 1677
Size: 555 1122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1677
Size: 66 1611
AflIII A'CryG_T
Cuts at: 0 1020 1029 1055 1677
Size: 1020 9 26 622
Fragments arranged by size:
1020 622 26 9
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 412 1677
Size: 412 1265
AlwNI CAG_nnn'CTG
Cuts at: 0 754 1569 1677
Size: 754 815 108
Fragments arranged by size:
815 754 108
ApoI r'AATT_y
Cuts at: 0 7 965 1126 1134 1268 1364 1677
Size: 7 958 161 8 134 96 313
Fragments arranged by size:
958 313 161 134 96 8 7
AseI AT'TA_AT
Cuts at: 0 419 1677
Size: 419 1258
BanI G'GyrC_C
Cuts at: 0 1238 1599 1677
Size: 1238 361 78
Fragments arranged by size:
1238 361 78
BciVI GTATCCnnnnn_n'
Cuts at: 0 341 812 1677
Size: 341 471 865
Fragments arranged by size:
865 471 341
BclI T'GATC_A
Cuts at: 0 566 611 719 1677
Size: 566 45 108 958
Fragments arranged by size:
958 566 108 45
BglII A'GATC_T
Cuts at: 0 286 1677
Size: 286 1391
Bme1580I G_kGCm'C
Cuts at: 0 1604 1677
Size: 1604 73
BmgBI CAC'GTC
Cuts at: 0 1276 1677
Size: 1276 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 1465 1522 1677
Size: 1465 57 155
Fragments arranged by size:
1465 155 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 194 676 1139 1677
Size: 152 42 482 463 538
Fragments arranged by size:
538 482 463 152 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 578 608 1677
Size: 578 30 1069
Fragments arranged by size:
1069 578 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 965 1677
Size: 965 712
BseYI C'CCAG_C
Cuts at: 0 1603 1634 1677
Size: 1603 31 43
Fragments arranged by size:
1603 43 31
BsiHKAI G_wGCw'C
Cuts at: 0 391 1677
Size: 391 1286
BsmI GAATG_Cn'
Cuts at: 0 94 1677
Size: 94 1583
Bsp1286I G_dGCh'C
Cuts at: 0 391 1604 1677
Size: 391 1213 73
Fragments arranged by size:
1213 391 73
BspHI T'CATG_A
Cuts at: 0 162 1677
Size: 162 1515
BsrDI GCAATG_nn'
Cuts at: 0 1626 1677
Size: 1626 51
BsrGI T'GTAC_A
Cuts at: 0 640 1677
Size: 640 1037
BstBI TT'CG_AA
Cuts at: 0 543 1650 1677
Size: 543 1107 27
Fragments arranged by size:
1107 543 27
BstYI r'GATC_y
Cuts at: 0 286 1677
Size: 286 1391
BtgI C'CryG_G
Cuts at: 0 869 1377 1677
Size: 869 508 300
Fragments arranged by size:
869 508 300
BtsI GCAGTG_nn'
Cuts at: 0 991 1645 1677
Size: 991 654 32
Fragments arranged by size:
991 654 32
ClaI AT'CG_AT
Cuts at: 0 713 1677
Size: 713 964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 338 373 1677
Size: 338 35 1304
Fragments arranged by size:
1304 338 35
DraI TTT'AAA
Cuts at: 0 237 1677
Size: 237 1440
EaeI y'GGCC_r
Cuts at: 0 668 1677
Size: 668 1009
EarI CTCTTCn'nnn_
Cuts at: 0 854 1580 1677
Size: 854 726 97
Fragments arranged by size:
854 726 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 152 194 676 1139 1677
Size: 66 86 42 482 463 538
Fragments arranged by size:
538 482 463 86 66 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 164 1677
Size: 164 1513
EcoO109I rG'GnC_Cy
Cuts at: 0 169 1677
Size: 169 1508
EcoRI G'AATT_C
Cuts at: 0 1126 1677
Size: 1126 551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1155 1677
Size: 1155 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1677
Size: 1174 32 471
Fragments arranged by size:
1174 471 32
KpnI G_GTAC'C
Cuts at: 0 1242 1677
Size: 1242 435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1005 1341 1677
Size: 1005 336 336
Fragments arranged by size:
1005 336 336
MscI TGG'CCA
Cuts at: 0 670 1677
Size: 670 1007
MslI CAynn'nnrTG
Cuts at: 0 715 1677
Size: 715 962
NcoI C'CATG_G
Cuts at: 0 869 1377 1677
Size: 869 508 300
Fragments arranged by size:
869 508 300
NdeI CA'TA_TG
Cuts at: 0 924 1677
Size: 924 753
NspI r_CATG'y
Cuts at: 0 1016 1024 1033 1552 1677
Size: 1016 8 9 519 125
Fragments arranged by size:
1016 519 125 9 8
PciI A'CATG_T
Cuts at: 0 1020 1029 1677
Size: 1020 9 648
Fragments arranged by size:
1020 648 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 1383 1677
Size: 1383 294
PfoI T'CCnGG_A
Cuts at: 0 171 620 1062 1677
Size: 171 449 442 615
Fragments arranged by size:
615 449 442 171
PpuMI rG'GwC_Cy
Cuts at: 0 169 1677
Size: 169 1508
PstI C_TGCA'G
Cuts at: 0 1547 1677
Size: 1547 130
SfcI C'TryA_G
Cuts at: 0 1543 1677
Size: 1543 134
SspI AAT'ATT
Cuts at: 0 1673 1677
Size: 1673 4
StuI AGG'CCT
Cuts at: 0 495 881 1677
Size: 495 386 796
Fragments arranged by size:
796 495 386
StyI C'CwwG_G
Cuts at: 0 869 1377 1677
Size: 869 508 300
Fragments arranged by size:
869 508 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1619 1677
Size: 1619 58
TatI w'GTAC_w
Cuts at: 0 150 640 740 1677
Size: 150 490 100 937
Fragments arranged by size:
937 490 150 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 1677
Size: 578 32 1067
Fragments arranged by size:
1067 578 32
XbaI T'CTAG_A
Cuts at: 0 141 1326 1677
Size: 141 1185 351
Fragments arranged by size:
1185 351 141
XmnI GAAnn'nnTTC
Cuts at: 0 452 1138 1677
Size: 452 686 539
Fragments arranged by size:
686 539 452
Enzymes that do cut:
Acc65I AclI AcuI AflIII AlfI AlwNI ApoI AseI
BanI BciVI BclI BglII Bme1580I BmgBI BmrI BpmI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BsrGI BstBI BstYI BtgI BtsI ClaI CspCI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
KpnI MmeI MscI MslI NcoI NdeI NspI PciI
PflMI PfoI PpuMI PstI SfcI SspI StuI StyI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbsI BbvCI BcgI BfrBI BglI
BlpI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgZI DraIII DrdI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MspA1I NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI Tth111I XcmI XhoI XmaI ZraI