################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsiWI BsmI BspDI BspHI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SacI SacII ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI TfiI XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 328 GTCGAC GT^[AC][GT]AC AccIII 1025 TCCGGA T^CCGGA AcsI 553 GAATTT [GA]^AATT[CT] AocI 292 CCTAAGG CC^T[ACGT]AGG Aor51HI 1352 AGCGCT AGC^GCT ApoI 553 GAATTT [GA]^AATT[CT] Asp700I 1617 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BsaAI 1141 TACGTG [CT]AC^GT[GA] BseAI 1025 TCCGGA T^CCGGA Bsp1407I 20 TGTACA T^GTACA BspEI 1025 TCCGGA T^CCGGA BsrDI 449 GCAATG GCAATG(2/0) BsrGI 20 TGTACA T^GTACA BstEII 2133 GGTGACC G^GT[ACGT]ACC BstPI 2133 GGTGACC G^GT[ACGT]ACC Bsu36I 292 CCTAAGG CC^T[ACGT]AGG DrdI 463 GACCCCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2360 CGGCCG C^GGCCG EclXI 2360 CGGCCG C^GGCCG Eco47III 1352 AGCGCT AGC^GCT Eco52I 2360 CGGCCG C^GGCCG Eco81I 292 CCTAAGG CC^T[ACGT]AGG EcoO65I 2133 GGTGACC G^GT[ACGT]ACC HincII 328 GTCGAC GT[CT]^[GA]AC HindII 328 GTCGAC GT[CT]^[GA]AC MroI 1025 TCCGGA T^CCGGA MslI 1372 CACGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 575 CCATGG C^CATGG NruI 1808 TCGCGA TCG^CGA NspI 848 GCATGC [GA]CATG^[CT] PshAI 321 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 509 CGATCG CGAT^CG SalI 328 GTCGAC G^TCGAC SbfI 2139 CCTGCAGG CCTGCA^GG SmaI 389 CCCGGG CCC^GGG SphI 848 GCATGC GCATG^C Sse8387I 2139 CCTGCAGG CCTGCA^GG SspBI 20 TGTACA T^GTACA StuI 596 AGGCCT AGG^CCT Tsp509I 554 AATT ^AATT XmaCI 389 CCCGGG C^CCGGG XmaI 389 CCCGGG C^CCGGG XmnI 1617 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Alw44I 175 GTGCAC G^TGCAC 817 GTGCAC ApaLI 175 GTGCAC G^TGCAC 817 GTGCAC AspI 610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2121 GACCCGGTC AvaI 389 CCCGGG C^[CT]CG[GA]G 2293 CCCGAG BbeI 1363 GGCGCC GGCGC^C 1692 GGCGCC BbvCI 231 CCTCAGC CCTCAGC(-5/-2) 1800 CCTCAGC BsaI 502 GAGACC GGTCTC(1/5) 1009 GAGACC BsaWI 1025 TCCGGA [AT]^CCGG[AT] 2026 ACCGGA BsiEI 509 CGATCG CG[GA][CT]^CG 2360 CGGCCG BsmBI 544 GAGACG CGTCTC(1/5) 781 GAGACG BsoBI 389 CCCGGG C^[CT]CG[GA]G 2293 CCCGAG BsrBI 1284 CCGCTC CCGCTC(-3/-3) 2244 GAGCGG BstYI 356 AGATCC [GA]^GATC[CT] 429 GGATCT KasI 1363 GGCGCC G^GCGCC 1692 GGCGCC MflI 356 AGATCC [GA]^GATC[CT] 429 GGATCT MseI 1172 TTAA T^TAA 1982 TTAA NarI 1363 GGCGCC GG^CGCC 1692 GGCGCC PflFI 610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2121 GACCCGGTC PpuMI 608 GGGACCT [GA]G^G[AT]CC[CT] 2330 AGGACCT SfoI 1363 GGCGCC GGC^GCC 1692 GGCGCC SmlI 931 CTCAAG C^T[CT][GA]AG 2173 CTTGAG Tru9I 1172 TTAA T^TAA 1982 TTAA Tth111I 610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2121 GACCCGGTC XcmI 959 CCAAGGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2384 CCAACTGCCTCCTGG XhoII 356 AGATCC [GA]^GATC[CT] 429 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I