Restriction map for
pFN21AE4228
(Circular) (Six-base) MAPSORT of: pFN21AE4228.seq Check: 5334 from: 1 to: 7114
pFN21AE4228 7114 bp
With 182 enzymes: SgfI *
March 28, 2012 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2193 2376 2835 3471 4212 2193
Size: 183 459 636 741 5095
Fragments arranged by size:
5095 741 636 459 183
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 4457 1937
Size: 397 2123 4594
Fragments arranged by size:
4594 2123 397
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 5241 5614 1563
Size: 3678 373 3063
Fragments arranged by size:
3678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3092 3641 5177 6561 6829 1632
Size: 1460 549 1536 1384 268 1917
Fragments arranged by size:
1917 1536 1460 1384 549 268
AfeI AGC'GCT
Cuts at: 3359 3359
Size: 7114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6917
AflIII A'CryG_T
Cuts at: 1203 6013 6990 1203
Size: 4810 977 1327
Fragments arranged by size:
4810 1327 977
AgeI A'CCGG_T
Cuts at: 5945 5945
Size: 7114
AhdI GACnn_n'nnGTC
Cuts at: 5843 5843
Size: 7114
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2681 2713 2681
Size: 32 7082
AlwNI CAG_nnn'CTG
Cuts at: 2668 2818 2959 3094 3250 3997 4857 6429 2668
Size: 150 141 135 156 747 860 1572 3353
Fragments arranged by size:
3353 1572 860 747 156 150 141 135
ApaI G_GGCC'C
Cuts at: 2320 2395 2864 3113 3271 3752 2320
Size: 75 469 249 158 481 5682
Fragments arranged by size:
5682 481 469 249 158 75
ApaLI G'TGCA_C
Cuts at: 2180 2822 5171 6327 2180
Size: 642 2349 1156 2967
Fragments arranged by size:
2967 2349 1156 642
ApoI r'AATT_y
Cuts at: 1493 2558 4423 4649 4801 6910 1493
Size: 1065 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 1065 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5668 160
Size: 872 4636 1606
Fragments arranged by size:
4636 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 2394 4298 4439 1870
Size: 73 451 1904 141 4545
Fragments arranged by size:
4545 1904 451 141 73
BamHI G'GATC_C
Cuts at: 4444 4811 4444
Size: 367 6747
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3368 3697 4200 4236 4273
Size: 325 217 627 1581 329 503 36 37
Cuts at: 4273 4435 5890 618
Size: 162 1455 1842
Fragments arranged by size:
1842 1581 1455 627 503 329 325 217
162 37 36
BanII G_rGCy'C
Cuts at: 1391 2118 2320 2395 2756 2864 3113 3271 3752
Size: 727 202 75 361 108 249 158 481
Cuts at: 3752 4046 4286 4389 4433 1391
Size: 294 240 103 44 4072
Fragments arranged by size:
4072 727 481 361 294 249 240 202
158 108 103 75 44
BbeI G_GCGC'C
Cuts at: 3372 3701 3372
Size: 329 6785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 3824 4054 4109 928
Size: 971 251 1674 230 55 3933
Fragments arranged by size:
3933 1674 971 251 230 55
BbvCI CC'TCA_GC
Cuts at: 2237 3806 2237
Size: 1569 5545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5305 5339 1903
Size: 34 3368 34 3678
Fragments arranged by size:
3678 3368 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 6216 5006
Size: 1210 5904
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2585 3569 4368
Size: 107 122 71 631 605 913 984 799
Cuts at: 4368 5725 136
Size: 1357 1525
Fragments arranged by size:
1525 1357 984 913 799 631 605 122
107 71
BglII A'GATC_T
Cuts at: 7109 7109
Size: 7114
BlpI GC'TnA_GC
Cuts at: 3275 3284 4247 4531 3275
Size: 9 963 284 5858
Fragments arranged by size:
5858 963 284 9
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2184 2320 2395 2826 2864 3113 3271
Size: 366 28 136 75 431 38 249 158
Cuts at: 3271 3308 3752 4278 5175 6331 1790
Size: 37 444 526 897 1156 2573
Fragments arranged by size:
2573 1156 897 526 444 431 366 249
158 136 75 38 37 28
BmgBI CAC'GTC
Cuts at: 1318 2920 3910 1318
Size: 1602 990 4522
Fragments arranged by size:
4522 1602 990
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2620 4845 5803
Size: 416 53 83 399 24 1203 2225 958
Cuts at: 5803 442
Size: 1753
Fragments arranged by size:
2225 1753 1203 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3057 3057
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 2785 2786 3014 3251 4342 4421 5774
Size: 1205 341 1 228 237 1091 79 1353
Cuts at: 5774 1239
Size: 2579
Fragments arranged by size:
2579 1353 1205 1091 341 237 228 79
1
Bpu10I CC'TnA_GC
Cuts at: 2237 3806 2237
Size: 1569 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 4199 5239 6104 6402 2921
Size: 1278 1040 865 298 3633
Fragments arranged by size:
3633 1278 1040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2501 3008 5777 882
Size: 617 1002 507 2769 2219
Fragments arranged by size:
2769 2219 1002 617 507
BsaAI yAC'GTr
Cuts at: 493 1204 3148 4905 493
Size: 711 1944 1757 2702
Fragments arranged by size:
2702 1944 1757 711
BsaBI GATnn'nnATC
Cuts at: 7108 7108
Size: 7114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3102 3369 3483 3698
Size: 53 83 186 936 1569 267 114 215
Cuts at: 3698 3821 3936 4059 4921 5303 5938 275
Size: 123 115 123 862 382 635 1451
Fragments arranged by size:
1569 1451 936 862 635 382 267 215
186 123 123 115 114 83 53
BsaWI w'CCGG_w
Cuts at: 3030 4031 5547 5945 6219 6366 6777 3030
Size: 1001 1516 398 274 147 411 3367
Fragments arranged by size:
3367 1516 1001 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 2681 2711 2738
Size: 30 165 30 1173 30 75 30 27
Cuts at: 2738 2768 3128 3158 1178
Size: 30 360 30 5134
Fragments arranged by size:
5134 1173 360 165 75 30 30 30
30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2360 2702 2903 3149 3668 3754 1186
Size: 997 177 342 201 246 519 86 4546
Fragments arranged by size:
4546 997 519 342 246 201 177 86
BseYI C'CCAG_C
Cuts at: 2848 3161 3372 3824 4039 4185 4207 6317 6813
Size: 313 211 452 215 146 22 2110 496
Cuts at: 6813 2848
Size: 3149
Fragments arranged by size:
3149 2110 496 452 313 215 211 146
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 2699 3080 3185 1163
Size: 573 636 327 381 105 5092
Fragments arranged by size:
5092 636 573 381 327 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2517 4368 4564 5325 6353 1540
Size: 275 186 516 1851 196 761 1028 2301
Fragments arranged by size:
2301 1851 1028 761 516 275 196 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2826 3194 5175 5260 6331 2184
Size: 642 368 1981 85 1071 2967
Fragments arranged by size:
2967 1981 1071 642 368 85
BsmI GAATG_Cn'
Cuts at: 4625 4718 4625
Size: 93 7021
BsmBI CGTCTCn'nnnn_
Cuts at: 2543 2780 2543
Size: 237 6877
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2156 2184 2320 2395 2756 2826
Size: 399 328 38 28 136 75 361 70
Cuts at: 2826 2864 3113 3194 3271 3308 3752 4046 4278
Size: 38 249 81 77 37 444 294 232
Cuts at: 4278 4286 4389 4433 5175 5260 6331 1391
Size: 8 103 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 444 399 361 328 294
249 232 136 103 85 81 77 75
70 44 38 38 37 28 8
BspEI T'CCGG_A
Cuts at: 3030 6777 3030
Size: 3747 3367
BspHI T'CATG_A
Cuts at: 5003 6733 5003
Size: 1730 5384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 2376 2835 2940 2988 3102 3471 3681
Size: 1349 183 459 105 48 114 369 210
Cuts at: 3681 3907 3969 4212 4469 844
Size: 226 62 243 257 3489
Fragments arranged by size:
3489 1349 459 369 257 243 226 210
183 114 105 62 48
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 4251 5001 1394
Size: 429 1468 960 750 3507
Fragments arranged by size:
3507 1468 960 750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 5609 5783 65
Size: 1183 1213 3148 174 1396
Fragments arranged by size:
3148 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2403 2727 3550 3938 4197 4367 5758
Size: 110 593 324 823 388 259 170 1391
Cuts at: 5758 5945 1700
Size: 187 2869
Fragments arranged by size:
2869 1391 823 593 388 324 259 187
170 110
BsrGI T'GTAC_A
Cuts at: 96 2025 96
Size: 1929 5185
BssHII G'CGCG_C
Cuts at: 1925 3240 3253 3279 3319 1925
Size: 1315 13 26 40 5720
Fragments arranged by size:
5720 1315 40 26 13
BssSI C'ACGA_G
Cuts at: 2837 3377 3641 4279 5174 6186 2837
Size: 540 264 638 895 1012 3765
Fragments arranged by size:
3765 1012 895 638 540 264
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3094 3250 3913 794
Size: 2300 156 663 3995
Fragments arranged by size:
3995 2300 663 156
BstBI TT'CG_AA
Cuts at: 5918 5918
Size: 7114
BstEII G'GTnAC_C
Cuts at: 1184 4138 1184
Size: 2954 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 3169 3643 2437
Size: 732 474 5908
Fragments arranged by size:
5908 732 474
BstYI r'GATC_y
Cuts at: 1972 2361 2434 4444 4811 5196 5213 6665 6751
Size: 389 73 2010 367 385 17 1452 86
Cuts at: 6751 6763 7109 1972
Size: 12 346 1977
Fragments arranged by size:
2010 1977 1452 389 385 367 346 86
73 17 12
Bsu36I CC'TnA_GG
Cuts at: 2298 2298
Size: 7114
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 3057 3312 4066 513
Size: 552 939 576 477 255 754 3561
Fragments arranged by size:
3561 939 754 576 552 477 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3379 487
Size: 683 56 2153 4222
Fragments arranged by size:
4222 2153 683 56
BtsI GCAGTG_nn'
Cuts at: 3971 4630 5424 5444 3971
Size: 659 794 20 5641
Fragments arranged by size:
5641 794 659 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3039 3074 529
Size: 35 2475 35 4569
Fragments arranged by size:
4569 2475 35 35
DraI TTT'AAA
Cuts at: 4418 4418
Size: 7114
DraIII CAC_nnn'GTG
Cuts at: 2139 2206 2907 2139
Size: 67 701 6346
Fragments arranged by size:
6346 701 67
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2474 6121 809
Size: 507 1158 3647 1802
Fragments arranged by size:
3647 1802 1158 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2384 2922
Size: 54 1425 50 275 18 177 377 538
Cuts at: 2922 2961 3078 3513 4365 4561 5450 7079 8
Size: 39 117 435 852 196 889 1629 43
Fragments arranged by size:
1629 1425 889 852 538 435 377 275
196 177 117 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 4365 4561 1537
Size: 275 2553 196 4090
Fragments arranged by size:
4090 2553 275 196
EarI CTCTTCn'nnn_
Cuts at: 1353 2948 2990 3344 3461 3728 5044 1353
Size: 1595 42 354 117 267 1316 3423
Fragments arranged by size:
3423 1595 1316 354 267 117 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3320 5701 6075 6221 387
Size: 1064 138 1731 2381 374 146 1280
Fragments arranged by size:
2381 1731 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 2785 2786 3014 3092 3251 3641
Size: 393 812 341 1 228 78 159 390
Cuts at: 3641 4342 4421 5177 5774 6561 6829 1239
Size: 701 79 756 597 787 268 1524
Fragments arranged by size:
1524 812 787 756 701 597 393 390
341 268 228 159 79 78 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2327 2951 4343 2327
Size: 624 1392 5098
Fragments arranged by size:
5098 1392 624
EcoO109I rG'GnC_Cy
Cuts at: 2391 2614 2860 3110 3267 3268 3748 3749 3802
Size: 223 246 250 157 1 480 1 53
Cuts at: 3802 3945 4336 2391
Size: 143 391 5169
Fragments arranged by size:
5169 480 391 250 246 223 157 143
53 1 1
EcoRI G'AATT_C
Cuts at: 4423 4801 4423
Size: 378 6736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3799 3799
Size: 7114
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 2591 3361 3372 3475 3701 3843 6261 1678
Size: 913 770 11 103 226 142 2418 2531
Fragments arranged by size:
2531 2418 913 770 226 142 103 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5755 5787 5829 5861 1373
Size: 32 135 32 4183 32 42 32 2626
Fragments arranged by size:
4183 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 4458 4704 669
Size: 988 281 397 2123 246 3079
Fragments arranged by size:
3079 2123 988 397 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7114
HpaI GTT'AAC
Cuts at: 4704 4704
Size: 7114
KasI G'GCGC_C
Cuts at: 3368 3697 3368
Size: 329 6785
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 4713 4713
Size: 7114
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6228 6412 1305
Size: 4923 184 2007
Fragments arranged by size:
4923 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2386 2924 2963 3515
Size: 54 1425 343 177 377 538 39 552
Cuts at: 3515 10
Size: 3609
Fragments arranged by size:
3609 1425 552 538 377 343 177 54
39
MslI CAynn'nnrTG
Cuts at: 518 1166 3381 5072 5431 5590 518
Size: 648 2215 1691 359 159 2042
Fragments arranged by size:
2215 2042 1691 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2310 2764 2872 2992 3586 3619 3679 3872
Size: 704 454 108 120 594 33 60 193
Cuts at: 3872 4530 5207 6355 6600 1606
Size: 658 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 658 594 454 245
193 120 108 60 33
NaeI GCC'GGC
Cuts at: 2405 2729 3552 3940 4369 2405
Size: 324 823 388 429 5150
Fragments arranged by size:
5150 823 429 388 324
NarI GG'CG_CC
Cuts at: 3369 3698 3369
Size: 329 6785
NcoI C'CATG_G
Cuts at: 513 1065 2004 2580 513
Size: 552 939 576 5047
Fragments arranged by size:
5047 939 576 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7114
NgoMIV G'CCGG_C
Cuts at: 2403 2727 3550 3938 4367 2403
Size: 324 823 388 429 5150
Fragments arranged by size:
5150 823 429 388 324
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NruI TCG'CGA
Cuts at: 3815 3815
Size: 7114
NspI r_CATG'y
Cuts at: 1132 1229 2857 4472 6017 1132
Size: 97 1628 1615 1545 2229
Fragments arranged by size:
2229 1628 1615 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2864 1348
Size: 1516 5598
PciI A'CATG_T
Cuts at: 6013 6013
Size: 7114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2139 2206 2818 2920 3532 3610 4227 1108
Size: 1031 67 612 102 612 78 617 3995
Fragments arranged by size:
3995 1031 617 612 612 102 78 67
PfoI T'CCnGG_A
Cuts at: 2973 4398 2973
Size: 1425 5689
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2713 5183 5215 6696 6728 2681
Size: 32 2470 32 1481 32 3067
Fragments arranged by size:
3067 2470 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2614 4336 2614
Size: 1722 5392
PshAI GACnn'nnGTC
Cuts at: 1613 2330 1613
Size: 717 6397
PsiI TTA'TAA
Cuts at: 4684 4684
Size: 7114
PspOMI G'GGCC_C
Cuts at: 2316 2391 2860 3109 3267 3748 2316
Size: 75 469 249 158 481 5682
Fragments arranged by size:
5682 481 469 249 158 75
PstI C_TGCA'G
Cuts at: 830 2571 2709 3591 3678 3966 4149 4335 4410
Size: 1741 138 882 87 288 183 186 75
Cuts at: 4410 4466 830
Size: 56 3478
Fragments arranged by size:
3478 1741 882 288 186 183 138 87
75 56
PvuI CG_AT'CG
Cuts at: 2001 2517 2001
Size: 516 6598
PvuII CAG'CTG
Cuts at: 2764 2872 2992 3619 2764
Size: 108 120 627 6259
Fragments arranged by size:
6259 627 120 108
SalI G'TCGA_C
Cuts at: 1936 2333 4456 1936
Size: 397 2123 4594
Fragments arranged by size:
4594 2123 397
SapI GCTCTTCn'nnn_
Cuts at: 2948 2990 3344 3461 2948
Size: 42 354 117 6601
Fragments arranged by size:
6601 354 117 42
SbfI CC_TGCA'GG
Cuts at: 4149 4466 4149
Size: 317 6797
ScaI AGT'ACT
Cuts at: 5362 7074 5362
Size: 1712 5402
SfcI C'TryA_G
Cuts at: 826 1045 2567 2705 3587 3674 3962 4145 4331
Size: 219 1522 138 882 87 288 183 186
Cuts at: 4331 4406 4462 5597 6278 6469 826
Size: 75 56 1135 681 191 1471
Fragments arranged by size:
1522 1471 1135 882 681 288 219 191
186 183 138 87 75 56
SfoI GGC'GCC
Cuts at: 3370 3699 3370
Size: 329 6785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7114
SmaI CCC'GGG
Cuts at: 1872 2396 4441 1872
Size: 524 2045 4545
Fragments arranged by size:
4545 2045 524
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 4178 5218 6119 6381 820
Size: 197 926 993 1242 1040 901 262 1553
Fragments arranged by size:
1553 1242 1040 993 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 2857 4472 1132
Size: 1725 1615 3774
Fragments arranged by size:
3774 1725 1615
SrfI GCCC'GGGC
Cuts at: 2396 2396
Size: 7114
SspI AAT'ATT
Cuts at: 5 52 5038 5
Size: 47 4986 2081
Fragments arranged by size:
4986 2081 47
StuI AGG'CCT
Cuts at: 1517 2603 1517
Size: 1086 6028
StyI C'CwwG_G
Cuts at: 513 1065 2004 2061 2580 2964 3516 3564 3738
Size: 552 939 57 519 384 552 48 174
Cuts at: 3738 4553 513
Size: 815 3074
Fragments arranged by size:
3074 939 815 552 552 519 384 174
57 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3230 5141 5158 5311 5496 5941 325
Size: 1255 1650 1911 17 153 185 445 1498
Fragments arranged by size:
1911 1650 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 5360 7072
Size: 274 80 33 51 1443 48 3335 1712
Cuts at: 7072 96
Size: 138
Fragments arranged by size:
3335 1712 1443 274 138 80 51 48
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2576 2608 3173 3205 3711 3743 7074 7106 2576
Size: 32 565 32 506 32 3331 32 2584
Fragments arranged by size:
3331 2584 565 506 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2618 4129 1316
Size: 1302 1511 4301
Fragments arranged by size:
4301 1511 1302
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2971 4396 2971
Size: 1425 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7114
XmaI C'CCGG_G
Cuts at: 1870 2394 4439 1870
Size: 524 2045 4545
Fragments arranged by size:
4545 2045 524
XmnI GAAnn'nnTTC
Cuts at: 3626 5243 3626
Size: 1617 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV
FseI FspAI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SwaI