Restriction map for
pFN21AB3205
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BfrI BglII BlnI BsaBI
BsaHI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspMI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MunI MvnI NarI NcoI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 797 GAATTC [GA]^AATT[CT]
AflIII 115 ACATGT A^C[GA][CT]GT
AlwI 1353 GATCC GGATC(4/5)
ApaI 983 GGGCCC GGGCC^C
ApoI 797 GAATTC [GA]^AATT[CT]
BanI 1221 GGTGCC G^G[CT][GA]CC
BbvCI 1213 CCTCAGC CCTCAGC(-5/-2)
BcgI 1185 GCAAGTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 422 GTATCC GTATCC(6/5)
BsaAI 680 CACGTA [CT]AC^GT[GA]
BsaI 1426 GAGACC GGTCTC(1/5)
BsgI 1113 CTGCAC GTGCAG(16/14)
BspHI 653 TCATGA T^CATGA
BspLU11I 115 ACATGT A^CATGT
BsrFI 1601 GCCGGC [GA]^CCGG[CT]
BstAPI 342 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1356 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 690 GCGC GCG^C
Cfr10I 1601 GCCGGC [GA]^CCGG[CT]
DrdI 631 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 797 GAATTC G^AATTC
EcoRV 103 GATATC GAT^ATC
HaeII 689 GGCGCT [GA]GCGC^[CT]
HhaI 690 GCGC GCG^C
HinP1I 690 GCGC G^CGC
HincII 430 GTCGAC GT[CT]^[GA]AC
HindII 430 GTCGAC GT[CT]^[GA]AC
MaeII 681 ACGT A^CGT
MseI 659 TTAA T^TAA
NaeI 1601 GCCGGC GCC^GGC
NdeI 1482 CATATG CA^TATG
NgoMIV 1601 GCCGGC G^CCGGC
NheI 1135 GCTAGC G^CTAGC
NspI 115 ACATGT [GA]CATG^[CT]
PflMI 1225 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 123 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 125 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1507 CTGCAG CTGCA^G
PvuII 1216 CAGCTG CAG^CTG
RcaI 653 TCATGA T^CATGA
SalI 430 GTCGAC G^TCGAC
Tru9I 659 TTAA T^TAA
Van91I 1225 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1283 CCATGACTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 430 GTCGAC GT^[AC][GT]AC
1382 GTCTAC
AccIII 52 TCCGGA T^CCGGA
944 TCCGGA
BalI 1303 TGGCCA TGG^CCA
1626 TGGCCA
BanII 983 GGGCCC G[GA]GC[CT]^C
1613 GGGCTC
BbsI 628 GAAGAC GAAGAC(2/6)
948 GAAGAC
BclI 365 TGATCA T^GATCA
398 TGATCA
BcnI 986 CCCGG CC^[GC]GG
1128 CCGGG
BglI 976 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1601 GCCGGCAAGGC
BpuAI 628 GAAGAC GAAGAC(2/6)
948 GAAGAC
BsaWI 52 TCCGGA [AT]^CCGG[AT]
944 TCCGGA
BseAI 52 TCCGGA T^CCGGA
944 TCCGGA
BsmBI 1447 GAGACG CGTCTC(1/5)
1531 GAGACG
BsmFI 123 GGGAC GGGAC(10/14)
280 GGGAC
BspEI 52 TCCGGA T^CCGGA
944 TCCGGA
BssSI 776 CACGAG CACGAG(-5/-1)
22 CTCGTG
EarI 265 GAAGAG CTCTTC(1/4)
814 GAAGAG
EcoT14I 1106 CCAAGG C^C[AT][AT]GG
1277 CCAAGG
FauI 915 GCGGG CCCGC(4/6)
1610 GCGGG
FbaI 365 TGATCA T^GATCA
398 TGATCA
Ksp632I 265 GAAGAG CTCTTC(1/4)
814 GAAGAG
MaeIII 440 GTGAC ^GT[ACGT]AC
670 GTGAC
MluNI 1303 TGGCCA TGG^CCA
1626 TGGCCA
MroI 52 TCCGGA T^CCGGA
944 TCCGGA
MscI 1303 TGGCCA TGG^CCA
1626 TGGCCA
MslI 168 CATTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1182 CATGCAAGTG
MspA1I 224 CAGCGG C[AC]G^C[GT]G
1216 CAGCTG
NciI 986 CCCGG CC^[GC]GG
1128 CCGGG
SfcI 320 CTGTAG C^T[GA][CT]AG
1507 CTGCAG
StuI 1041 AGGCCT AGG^CCT
1109 AGGCCT
StyI 1106 CCAAGG C^C[AT][AT]GG
1277 CCAAGG
Tsp45I 440 GTGAC ^GT[GC]AC
670 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I CelII
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI TseI Tsp509I
TspRI TthHB8I