Restriction map for
pFN21AB3205
(Linear) (Six-base) MAPSORT of: KIBB3205.seq Check: 2618 from: 1 to: 1635
KIBB3205.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 431 1383 1635
Size: 431 952 252
Fragments arranged by size:
952 431 252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1635
Size: 874 761
AflIII A'CryG_T
Cuts at: 0 115 1635
Size: 115 1520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 1635
Size: 733 902
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 47 79 1154 1186 1635
Size: 47 32 1075 32 449
Fragments arranged by size:
1075 449 47 32 32
ApaI G_GGCC'C
Cuts at: 0 987 1635
Size: 987 648
ApoI r'AATT_y
Cuts at: 0 797 1635
Size: 797 838
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1445 1478 1635
Size: 1445 33 157
Fragments arranged by size:
1445 157 33
BanI G'GyrC_C
Cuts at: 0 1221 1635
Size: 1221 414
BanII G_rGCy'C
Cuts at: 0 987 1617 1635
Size: 987 630 18
Fragments arranged by size:
987 630 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 955 1635
Size: 635 320 680
Fragments arranged by size:
680 635 320
BbvCI CC'TCA_GC
Cuts at: 0 1214 1635
Size: 1214 421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1174 1208 1635
Size: 1174 34 427
Fragments arranged by size:
1174 427 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 433 1635
Size: 433 1202
BclI T'GATC_A
Cuts at: 0 365 398 1635
Size: 365 33 1237
Fragments arranged by size:
1237 365 33
BglI GCCn_nnn'nGGC
Cuts at: 0 982 1607 1635
Size: 982 625 28
Fragments arranged by size:
982 625 28
BlpI GC'TnA_GC
Cuts at: 0 28 326 899 1635
Size: 28 298 573 736
Fragments arranged by size:
736 573 298 28
Bme1580I G_kGCm'C
Cuts at: 0 987 1635
Size: 987 648
BmrI ACTGGGnnnn_n'
Cuts at: 0 421 1486 1635
Size: 421 1065 149
Fragments arranged by size:
1065 421 149
BmtI G_CTAG'C
Cuts at: 0 1139 1635
Size: 1139 496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1167 1635
Size: 1167 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 635 787 1156 1635
Size: 358 277 152 369 479
Fragments arranged by size:
479 369 358 277 152
Bpu10I CC'TnA_GC
Cuts at: 0 1214 1635
Size: 1214 421
BsaI GGTCTCn'nnnn_
Cuts at: 0 1420 1635
Size: 1420 215
BsaAI yAC'GTr
Cuts at: 0 682 1635
Size: 682 953
BsaWI w'CCGG_w
Cuts at: 0 52 944 1635
Size: 52 892 691
Fragments arranged by size:
892 691 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 155 185 802 832 1154 1184 1635
Size: 155 30 617 30 322 30 451
Fragments arranged by size:
617 451 322 155 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 796 1114 1635
Size: 571 225 318 521
Fragments arranged by size:
571 521 318 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1098 1635
Size: 1098 537
BsiHKAI G_wGCw'C
Cuts at: 0 23 29 332 1635
Size: 23 6 303 1303
Fragments arranged by size:
1303 303 23 6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1441 1525 1635
Size: 1441 84 110
Fragments arranged by size:
1441 110 84
Bsp1286I G_dGCh'C
Cuts at: 0 23 29 332 987 1617 1635
Size: 23 6 303 655 630 18
Fragments arranged by size:
655 630 303 23 18 6
BspEI T'CCGG_A
Cuts at: 0 52 944 1635
Size: 52 892 691
Fragments arranged by size:
892 691 52
BspHI T'CATG_A
Cuts at: 0 653 1635
Size: 653 982
BsrDI GCAATG_nn'
Cuts at: 0 195 521 965 1203 1635
Size: 195 326 444 238 432
Fragments arranged by size:
444 432 326 238 195
BsrFI r'CCGG_y
Cuts at: 0 1601 1635
Size: 1601 34
BssSI C'ACGA_G
Cuts at: 0 22 776 1635
Size: 22 754 859
Fragments arranged by size:
859 754 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 348 1635
Size: 348 1287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1363 1635
Size: 1363 272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1604 1635
Size: 1604 31
BtsI GCAGTG_nn'
Cuts at: 0 1532 1635
Size: 1532 103
DrdI GACnn_nn'nnGTC
Cuts at: 0 637 1635
Size: 637 998
EaeI y'GGCC_r
Cuts at: 0 227 1303 1626 1635
Size: 227 1076 323 9
Fragments arranged by size:
1076 323 227 9
EarI CTCTTCn'nnn_
Cuts at: 0 260 809 1635
Size: 260 549 826
Fragments arranged by size:
826 549 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 635 787 874 1156 1635
Size: 358 277 152 87 282 479
Fragments arranged by size:
479 358 282 277 152 87
EcoO109I rG'GnC_Cy
Cuts at: 0 124 983 1235 1326 1635
Size: 124 859 252 91 309
Fragments arranged by size:
859 309 252 124 91
EcoRI G'AATT_C
Cuts at: 0 797 1635
Size: 797 838
EcoRV GAT'ATC
Cuts at: 0 105 1635
Size: 105 1530
HaeII r_GCGC'y
Cuts at: 0 693 1635
Size: 693 942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1635
Size: 26 1609
HincII GTy'rAC
Cuts at: 0 432 1635
Size: 432 1203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 547 873 1008 1028 1635
Size: 252 295 326 135 20 607
Fragments arranged by size:
607 326 295 252 135 20
MscI TGG'CCA
Cuts at: 0 1305 1628 1635
Size: 1305 323 7
Fragments arranged by size:
1305 323 7
MslI CAynn'nnrTG
Cuts at: 0 172 1186 1635
Size: 172 1014 449
Fragments arranged by size:
1014 449 172
MspA1I CmG'CkG
Cuts at: 0 226 1218 1635
Size: 226 992 417
Fragments arranged by size:
992 417 226
NaeI GCC'GGC
Cuts at: 0 1603 1635
Size: 1603 32
NdeI CA'TA_TG
Cuts at: 0 1483 1635
Size: 1483 152
NgoMIV G'CCGG_C
Cuts at: 0 1601 1635
Size: 1601 34
NheI G'CTAG_C
Cuts at: 0 1135 1635
Size: 1135 500
NspI r_CATG'y
Cuts at: 0 119 1635
Size: 119 1516
PciI A'CATG_T
Cuts at: 0 115 1635
Size: 115 1520
PflMI CCAn_nnn'nTGG
Cuts at: 0 1231 1635
Size: 1231 404
PfoI T'CCnGG_A
Cuts at: 0 335 1172 1205 1635
Size: 335 837 33 430
Fragments arranged by size:
837 430 335 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1154 1186 1635
Size: 1154 32 449
Fragments arranged by size:
1154 449 32
PpuMI rG'GwC_Cy
Cuts at: 0 124 1635
Size: 124 1511
PshAI GACnn'nnGTC
Cuts at: 0 129 1635
Size: 129 1506
PsiI TTA'TAA
Cuts at: 0 1517 1635
Size: 1517 118
PspOMI G'GGCC_C
Cuts at: 0 983 1635
Size: 983 652
PstI C_TGCA'G
Cuts at: 0 1511 1635
Size: 1511 124
PvuII CAG'CTG
Cuts at: 0 1218 1635
Size: 1218 417
SalI G'TCGA_C
Cuts at: 0 430 1635
Size: 430 1205
SanDI GG'GwC_CC
Cuts at: 0 124 1635
Size: 124 1511
SfcI C'TryA_G
Cuts at: 0 320 1507 1635
Size: 320 1187 128
Fragments arranged by size:
1187 320 128
StuI AGG'CCT
Cuts at: 0 1043 1111 1635
Size: 1043 68 524
Fragments arranged by size:
1043 524 68
StyI C'CwwG_G
Cuts at: 0 1106 1277 1635
Size: 1106 171 358
Fragments arranged by size:
1106 358 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 368 1426 1635
Size: 368 1058 209
Fragments arranged by size:
1058 368 209
TatI w'GTAC_w
Cuts at: 0 818 1635
Size: 818 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 155 187 1635
Size: 155 32 1448
Fragments arranged by size:
1448 155 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1290 1635
Size: 1290 345
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI ApaI ApoI BaeI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BsrDI BsrFI BssSI BstAPI BstXI BtgZI
BtsI DrdI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NdeI NgoMIV NheI NspI PciI PflMI PfoI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuII SalI SanDI
SfcI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BfrBI BglII BmgBI BpuEI BsaBI BsaHI
BseYI BsiEI BsiWI BsmI BspMI BsrBI BsrGI BssHII
BstBI BstEII BstYI BstZ17I Bsu36I BtgI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoNI FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NotI NruI NsiI PacI PasI PmeI
PmlI PspXI PsrI PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI