################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbeI BbrPI BbvCI BclI BfrI BglI BglII BlnI BsaAI BsaHI BsgI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FbaI FspI HaeII Hin1I KasI KspI MaeII MluI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 1239 CGCG CG^CG AccIII 1223 TCCGGA T^CCGGA AcsI 1468 GAATTC [GA]^AATT[CT] AhdI 833 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1684 GGATC GGATC(4/5) ApaI 53 GGGCCC GGGCC^C ApoI 1468 GAATTC [GA]^AATT[CT] AspEI 833 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcnI 1716 CCGGG CC^[GC]GG BsaBI 960 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1223 TCCGGA T^CCGGA BspEI 1223 TCCGGA T^CCGGA BspMI 1840 ACCTGC ACCTGC(4/8) BsrFI 2149 GCCGGC [GA]^CCGG[CT] BssSI 2101 CTCGTG CACGAG(-5/-1) BstUI 1239 CGCG CG^CG BstYI 1684 GGATCT [GA]^GATC[CT] Cfr10I 2149 GCCGGC [GA]^CCGG[CT] Eam1105I 833 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1468 GAATTC G^AATTC EcoRV 1705 GATATC GAT^ATC FauI 1885 CCCGC CCCGC(4/6) FseI 2148 GGCCGGCC GGCCGG^CC HgaI 561 GACGC GACGC(5/10) HindIII 1655 AAGCTT A^AGCTT HpaI 1081 GTTAAC GTT^AAC MamI 960 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 836 CAATTG C^AATTG MflI 1684 GGATCT [GA]^GATC[CT] MroI 1223 TCCGGA T^CCGGA MunI 836 CAATTG C^AATTG MvnI 1239 CGCG CG^CG NaeI 2149 GCCGGC GCC^GGC NciI 1716 CCGGG CC^[GC]GG NgoMIV 2149 GCCGGC G^CCGGC PaeR7I 1735 CTCGAG C^TCGAG ScaI 1739 AGTACT AGT^ACT SmlI 1735 CTCGAG C^T[CT][GA]AG StuI 496 AGGCCT AGG^CCT XhoI 1735 CTCGAG C^TCGAG XhoII 1684 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 302 GGTACC G^GTACC 1421 GGTACC Asp718I 302 GGTACC G^GTACC 1421 GGTACC AvaI 1615 CCCGAG C^[CT]CG[GA]G 1735 CTCGAG BcgI 2093 GCAACCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2168 GCAATTTCATCG BciVI 965 GTATCC GTATCC(6/5) 1275 GTATCC BsaWI 341 ACCGGA [AT]^CCGG[AT] 1223 TCCGGA BseRI 1050 CTCCTC GAGGAG(10/8) 662 GAGGAG BsmBI 2178 CGTCTC CGTCTC(1/5) 1726 GAGACG BsmI 577 GAATGC GAATGC(1/-1) 1776 GAATGC BsoBI 1615 CCCGAG C^[CT]CG[GA]G 1735 CTCGAG CfoI 1240 GCGC GCG^C 1893 GCGC EarI 2196 CTCTTC CTCTTC(1/4) 1018 GAAGAG HhaI 1240 GCGC GCG^C 1893 GCGC HinP1I 1240 GCGC G^CGC 1893 GCGC HincII 571 GTTGAC GT[CT]^[GA]AC 1081 GTTAAC HindII 571 GTTGAC GT[CT]^[GA]AC 1081 GTTAAC KpnI 302 GGTACC GGTAC^C 1421 GGTACC Ksp632I 2196 CTCTTC CTCTTC(1/4) 1018 GAAGAG PflMI 909 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2139 CCAGTCAGTGG PpuMI 199 GGGTCCC [GA]G^G[AT]CC[CT] 882 AGGACCC PstI 982 CTGCAG CTGCA^G 2067 CTGCAG TaqI 1415 TCGA T^CGA 1736 TCGA TfiI 30 GAATC G^A[AT]TC 756 GATTC TthHB8I 1415 TCGA T^CGA 1736 TCGA Van91I 909 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2139 CCAGTCAGTGG XcmI 905 CCAACCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2154 CCACTCCAACAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I CelII Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI