(Circular) (Six-base) MAPSORT of: pFN21AE4215.seq Check: 7720 from: 1 to: 6919 pFN21AE4215 6919 bp With 182 enzymes: SgfI * March 28, 2012 13:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4729 278 Size: 53 83 186 936 3193 2468 Fragments arranged by size: 3193 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4262 1937 Size: 2325 4594 Acc65I G'GTAC_C Cuts at: 2307 3426 4240 2307 Size: 1119 814 4986 Fragments arranged by size: 4986 1119 814 AclI AA'CG_TT Cuts at: 1563 5046 5419 1563 Size: 3483 373 3063 Fragments arranged by size: 3483 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2136 3042 3518 4039 4982 6366 6634 1632 Size: 504 906 476 521 943 1384 268 1917 Fragments arranged by size: 1917 1384 943 906 521 504 476 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6722 AflIII A'CryG_T Cuts at: 1203 5818 6795 1203 Size: 4615 977 1327 Fragments arranged by size: 4615 1327 977 AgeI A'CCGG_T Cuts at: 5750 5750 Size: 6919 AhdI GACnn_n'nnGTC Cuts at: 2843 5648 2843 Size: 2805 4114 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6919 AlwNI CAG_nnn'CTG Cuts at: 4662 6234 4662 Size: 1572 5347 ApaI G_GGCC'C Cuts at: 2062 2062 Size: 6919 ApaLI G'TGCA_C Cuts at: 4976 6132 4976 Size: 1156 5763 ApoI r'AATT_y Cuts at: 1493 3473 4228 4454 4606 6715 1493 Size: 1980 755 226 152 2109 1697 Fragments arranged by size: 2109 1980 1697 755 226 152 AseI AT'TA_AT Cuts at: 160 1032 5473 160 Size: 872 4441 1606 Fragments arranged by size: 4441 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 AvaI C'yCGr_G Cuts at: 1870 1943 3620 3740 4244 1870 Size: 73 1677 120 504 4545 Fragments arranged by size: 4545 1677 504 120 73 BamHI G'GATC_C Cuts at: 4249 4616 4249 Size: 367 6552 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2272 2307 2906 3426 4240 Size: 325 217 627 485 35 599 520 814 Cuts at: 4240 5695 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 814 627 599 520 485 325 217 35 BanII G_rGCy'C Cuts at: 1391 2062 2388 3110 3201 3420 3582 4238 1391 Size: 671 326 722 91 219 162 656 4072 Fragments arranged by size: 4072 722 671 656 326 219 162 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2009 2448 2858 4016 928 Size: 971 110 439 410 1158 3831 Fragments arranged by size: 3831 1158 971 439 410 110 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4087 4121 4162 4196 5110 5144 1903 Size: 34 2150 34 41 34 914 34 3678 Fragments arranged by size: 3678 2150 914 41 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2981 3291 4811 6021 2981 Size: 310 1520 1210 3879 Fragments arranged by size: 3879 1520 1210 310 BclI T'GATC_A Cuts at: 1548 1548 Size: 6919 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5530 136 Size: 107 122 71 631 605 3858 1525 Fragments arranged by size: 3858 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6914 6914 Size: 6919 BlpI GC'TnA_GC Cuts at: 3195 3200 3605 4336 3195 Size: 5 405 731 5778 Fragments arranged by size: 5778 731 405 5 Bme1580I G_kGCm'C Cuts at: 1790 2062 2277 4980 6136 1790 Size: 272 215 2703 1156 2573 Fragments arranged by size: 2703 2573 1156 272 215 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6919 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2253 3122 4138 Size: 416 53 83 399 24 836 869 1016 Cuts at: 4138 4650 5608 442 Size: 512 958 1753 Fragments arranged by size: 1753 1016 958 869 836 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6919 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2818 3094 3120 3792 5579 1239 Size: 1579 276 26 672 1787 2579 Fragments arranged by size: 2579 1787 1579 672 276 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5044 5909 6207 5044 Size: 865 298 5756 Fragments arranged by size: 5756 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2311 3058 3743 5582 882 Size: 617 812 747 685 1839 2219 Fragments arranged by size: 2219 1839 812 747 685 617 BsaAI yAC'GTr Cuts at: 493 1204 4710 493 Size: 711 3506 2702 Fragments arranged by size: 3506 2702 711 BsaBI GATnn'nnATC Cuts at: 2969 6913 2969 Size: 3944 2975 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4726 5108 5743 275 Size: 53 83 186 936 3193 382 635 1451 Fragments arranged by size: 3193 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2346 3228 5352 5750 6024 6171 6582 2346 Size: 882 2124 398 274 147 411 2683 Fragments arranged by size: 2683 2124 882 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6694 Fragments arranged by size: 6694 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2682 3046 1186 Size: 1496 364 5059 Fragments arranged by size: 5059 1496 364 BseYI C'CCAG_C Cuts at: 2055 2201 3044 3828 3876 6122 6618 2055 Size: 146 843 784 48 2246 496 2356 Fragments arranged by size: 2356 2246 843 784 496 146 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4369 5130 6158 1540 Size: 275 186 2368 761 1028 2301 Fragments arranged by size: 2368 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2945 3110 3201 3236 3582 4980 5065 6136 2945 Size: 165 91 35 346 1398 85 1071 3728 Fragments arranged by size: 3728 1398 1071 346 165 91 85 35 BsmI GAATG_Cn' Cuts at: 2588 3787 4430 4523 2588 Size: 1199 643 93 4984 Fragments arranged by size: 4984 1199 643 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3725 4189 3725 Size: 464 6455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2277 2388 2945 3110 3201 3236 Size: 399 272 215 111 557 165 91 35 Cuts at: 3236 3420 3582 4238 4980 5065 6136 1391 Size: 184 162 656 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 656 557 399 272 215 184 165 162 111 91 85 35 BspEI T'CCGG_A Cuts at: 3228 6582 3228 Size: 3354 3565 BspHI T'CATG_A Cuts at: 4808 6538 4808 Size: 1730 5189 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3854 4274 844 Size: 3010 420 3489 Fragments arranged by size: 3489 3010 420 BsrBI CCG'CTC Cuts at: 1394 1823 4806 1394 Size: 429 2983 3507 Fragments arranged by size: 3507 2983 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2148 2511 2596 3670 5414 5588 65 Size: 1183 900 363 85 1074 1744 174 1396 Fragments arranged by size: 1744 1396 1183 1074 900 363 174 85 BsrFI r'CCGG_y Cuts at: 1700 1810 4154 5563 5750 1700 Size: 110 2344 1409 187 2869 Fragments arranged by size: 2869 2344 1409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6919 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6919 BssSI C'ACGA_G Cuts at: 4106 4979 5991 4106 Size: 873 1012 5034 Fragments arranged by size: 5034 1012 873 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6919 BstBI TT'CG_AA Cuts at: 5723 5723 Size: 6919 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6919 BstXI CCAn_nnnn'nTGG Cuts at: 2069 3397 3980 2069 Size: 1328 583 5008 Fragments arranged by size: 5008 1328 583 BstYI r'GATC_y Cuts at: 1972 3689 4249 4616 5001 5018 6470 6556 6568 Size: 1717 560 367 385 17 1452 86 12 Cuts at: 6568 6914 1972 Size: 346 1977 Fragments arranged by size: 1977 1717 1452 560 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2068 2125 3685 513 Size: 552 939 64 57 1560 3747 Fragments arranged by size: 3747 1560 939 552 64 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6180 Fragments arranged by size: 6180 683 56 BtsI GCAGTG_nn' Cuts at: 4435 5229 5249 4435 Size: 794 20 6105 Fragments arranged by size: 6105 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6919 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6884 DraI TTT'AAA Cuts at: 4223 4223 Size: 6919 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5926 809 Size: 507 4610 1802 Fragments arranged by size: 4610 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 2177 2922 Size: 54 1425 50 275 18 331 16 745 Cuts at: 2922 3399 3996 4152 4156 4366 5255 6884 8 Size: 477 597 156 4 210 889 1629 43 Fragments arranged by size: 1629 1425 889 745 597 477 331 275 210 156 54 50 43 18 16 4 EagI C'GGCC_G Cuts at: 1537 1812 4366 1537 Size: 275 2554 4090 Fragments arranged by size: 4090 2554 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3018 4207 4849 1353 Size: 1665 1189 642 3423 Fragments arranged by size: 3423 1665 1189 642 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 5506 5880 6026 387 Size: 1064 138 2588 1329 374 146 1280 Fragments arranged by size: 2588 1329 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3108 3199 3580 3108 Size: 91 381 6447 Fragments arranged by size: 6447 381 91 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2818 3042 3094 3120 3518 3792 Size: 393 504 682 224 52 26 398 274 Cuts at: 3792 4039 4982 5579 6366 6634 1239 Size: 247 943 597 787 268 1524 Fragments arranged by size: 1524 943 787 682 597 504 398 393 274 268 247 224 52 26 EcoO109I rG'GnC_Cy Cuts at: 2205 2888 3493 3976 2205 Size: 683 605 483 5148 Fragments arranged by size: 5148 683 605 483 EcoRI G'AATT_C Cuts at: 3473 4228 4606 3473 Size: 755 378 5786 Fragments arranged by size: 5786 755 378 EcoRV GAT'ATC Cuts at: 3712 3712 Size: 6919 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3583 3583 Size: 6919 FseI GG_CCGG'CC Cuts at: 4158 4158 Size: 6919 FspI TGC'GCA Cuts at: 5425 5425 Size: 6919 HaeII r_GCGC'y Cuts at: 1678 6066 1678 Size: 4388 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 2829 2861 3017 Size: 32 135 32 452 32 773 32 156 Cuts at: 3017 3049 3097 3129 4190 4222 5560 5592 5634 Size: 32 48 32 1061 32 1338 32 42 Cuts at: 5634 5666 1373 Size: 32 2626 Fragments arranged by size: 2626 1338 1061 773 452 156 135 48 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2578 3088 4263 4509 669 Size: 988 281 640 510 1175 246 3079 Fragments arranged by size: 3079 1175 988 640 510 281 246 HindIII A'AGCT_T Cuts at: 748 3660 748 Size: 2912 4007 HpaI GTT'AAC Cuts at: 3088 4509 3088 Size: 1421 5498 KpnI G_GTAC'C Cuts at: 2311 3430 4244 2311 Size: 1119 814 4986 Fragments arranged by size: 4986 1119 814 MfeI C'AATT_G Cuts at: 2841 4518 2841 Size: 1677 5242 MluI A'CGCG_T Cuts at: 6795 6795 Size: 6919 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3306 4188 6033 6217 1305 Size: 2001 882 1845 184 2007 Fragments arranged by size: 2007 2001 1845 882 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2163 2179 2924 3401 3998 Size: 54 1425 343 331 16 745 477 597 Cuts at: 3998 10 Size: 2931 Fragments arranged by size: 2931 1425 745 597 477 343 331 54 16 MslI CAynn'nnrTG Cuts at: 518 1166 2067 2930 3444 4877 5236 5395 518 Size: 648 901 863 514 1433 359 159 2042 Fragments arranged by size: 2042 1433 901 863 648 514 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2992 3194 3522 3880 4217 4335 5012 Size: 449 937 202 328 358 337 118 677 Cuts at: 5012 6160 6405 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 449 358 337 328 245 202 118 NaeI GCC'GGC Cuts at: 4156 4156 Size: 6919 NcoI C'CATG_G Cuts at: 513 1065 2004 2068 2125 3685 513 Size: 552 939 64 57 1560 3747 Fragments arranged by size: 3747 1560 939 552 64 57 NdeI CA'TA_TG Cuts at: 387 387 Size: 6919 NgoMIV G'CCGG_C Cuts at: 4154 4154 Size: 6919 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6919 NotI GC'GGCC_GC Cuts at: 4366 4366 Size: 6919 NspI r_CATG'y Cuts at: 1132 1229 4277 5822 1132 Size: 97 3048 1545 2229 Fragments arranged by size: 3048 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2312 1348 Size: 964 5955 PciI A'CATG_T Cuts at: 5818 5818 Size: 6919 PflMI CCAn_nnn'nTGG Cuts at: 1108 2920 4150 1108 Size: 1812 1230 3877 Fragments arranged by size: 3877 1812 1230 PfoI T'CCnGG_A Cuts at: 2226 3855 2226 Size: 1629 5290 PmeI GTTT'AAAC Cuts at: 4223 4223 Size: 6919 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4988 5020 6501 6533 4988 Size: 32 1481 32 5374 Fragments arranged by size: 5374 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 2888 2205 Size: 683 6236 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6919 PsiI TTA'TAA Cuts at: 4489 4489 Size: 6919 PspOMI G'GGCC_C Cuts at: 2058 2058 Size: 6919 PstI C_TGCA'G Cuts at: 830 2991 4076 4271 830 Size: 2161 1085 195 3478 Fragments arranged by size: 3478 2161 1085 195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6919 PvuII CAG'CTG Cuts at: 2055 2992 3194 2055 Size: 937 202 5780 Fragments arranged by size: 5780 937 202 SacI G_AGCT'C Cuts at: 3110 3201 3582 3110 Size: 91 381 6447 Fragments arranged by size: 6447 381 91 SalI G'TCGA_C Cuts at: 1936 4261 1936 Size: 2325 4594 SanDI GG'GwC_CC Cuts at: 2205 2205 Size: 6919 SbfI CC_TGCA'GG Cuts at: 4271 4271 Size: 6919 ScaI AGT'ACT Cuts at: 3746 5167 6879 3746 Size: 1421 1712 3786 Fragments arranged by size: 3786 1712 1421 SfcI C'TryA_G Cuts at: 826 1045 2152 2377 2987 3451 4072 4267 5402 Size: 219 1107 225 610 464 621 195 1135 Cuts at: 5402 6083 6274 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1107 681 621 610 464 225 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6919 SmaI CCC'GGG Cuts at: 1872 4246 1872 Size: 2374 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3740 5023 5924 6186 820 Size: 197 926 1797 1283 901 262 1553 Fragments arranged by size: 1797 1553 1283 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4710 493 Size: 4217 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6919 SphI G_CATG'C Cuts at: 1132 4277 1132 Size: 3145 3774 SspI AAT'ATT Cuts at: 5 52 4843 5 Size: 47 4791 2081 Fragments arranged by size: 4791 2081 47 StuI AGG'CCT Cuts at: 1517 2503 1517 Size: 986 5933 StyI C'CwwG_G Cuts at: 513 1065 2004 2068 2125 3685 3979 4358 513 Size: 552 939 64 57 1560 294 379 3074 Fragments arranged by size: 3074 1560 939 552 379 294 64 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3842 4946 4963 5116 5301 5746 325 Size: 1255 2262 1104 17 153 185 445 1498 Fragments arranged by size: 2262 1498 1255 1104 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3744 5165 6877 Size: 274 80 33 51 1443 1767 1421 1712 Cuts at: 6877 96 Size: 138 Fragments arranged by size: 1767 1712 1443 1421 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2935 2967 6879 6911 2935 Size: 32 3912 32 2943 Fragments arranged by size: 3912 2943 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6919 XbaI T'CTAG_A Cuts at: 4255 4255 Size: 6919 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2917 4166 2917 Size: 1249 5670 XhoI C'TCGA_G Cuts at: 1943 3740 1943 Size: 1797 5122 XmaI C'CCGG_G Cuts at: 1870 4244 1870 Size: 2374 4545 XmnI GAAnn'nnTTC Cuts at: 5048 5048 Size: 6919 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4727 276 Size: 53 83 186 936 3193 2468 Fragments arranged by size: 3193 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI