Restriction map for
pFN21AE4215
(Linear) (Six-base) MAPSORT of: KIEE4215.seq Check: 4495 from: 1 to: 2214
KIEE4215.orf
With 182 enzymes: SgfI *
March 22, 2012 17:35 ..
Acc65I G'GTAC_C
Cuts at: 0 302 1421 2214
Size: 302 1119 793
Fragments arranged by size:
1119 793 302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1037 1513 2034 2214
Size: 131 906 476 521 180
Fragments arranged by size:
906 521 476 180 131
AhdI GACnn_n'nnGTC
Cuts at: 0 838 2214
Size: 838 1376
ApaI G_GGCC'C
Cuts at: 0 57 2214
Size: 57 2157
ApoI r'AATT_y
Cuts at: 0 1468 2214
Size: 1468 746
AvaI C'yCGr_G
Cuts at: 0 1615 1735 2214
Size: 1615 120 479
Fragments arranged by size:
1615 479 120
BanI G'GyrC_C
Cuts at: 0 267 302 901 1421 2214
Size: 267 35 599 520 793
Fragments arranged by size:
793 599 520 267 35
BanII G_rGCy'C
Cuts at: 0 57 383 1105 1196 1415 1577 2214
Size: 57 326 722 91 219 162 637
Fragments arranged by size:
722 637 326 219 162 91 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 4 443 853 2011 2214
Size: 4 439 410 1158 203
Fragments arranged by size:
1158 439 410 203 4
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2082 2116 2157 2191 2214
Size: 2082 34 41 34 23
Fragments arranged by size:
2082 41 34 34 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 976 1286 2214
Size: 976 310 928
Fragments arranged by size:
976 928 310
BlpI GC'TnA_GC
Cuts at: 0 1190 1195 1600 2214
Size: 1190 5 405 614
Fragments arranged by size:
1190 614 405 5
Bme1580I G_kGCm'C
Cuts at: 0 57 272 2214
Size: 57 215 1942
Fragments arranged by size:
1942 215 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 248 1117 2133 2214
Size: 248 869 1016 81
Fragments arranged by size:
1016 869 248 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1089 1115 1787 2214
Size: 813 276 26 672 427
Fragments arranged by size:
813 672 427 276 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 306 1053 1738 2214
Size: 306 747 685 476
Fragments arranged by size:
747 685 476 306
BsaBI GATnn'nnATC
Cuts at: 0 964 2214
Size: 964 1250
BsaWI w'CCGG_w
Cuts at: 0 341 1223 2214
Size: 341 882 991
Fragments arranged by size:
991 882 341
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 677 1041 2214
Size: 677 364 1173
Fragments arranged by size:
1173 677 364
BseYI C'CCAG_C
Cuts at: 0 50 196 1039 1823 1871 2214
Size: 50 146 843 784 48 343
Fragments arranged by size:
843 784 343 146 50 48
BsiHKAI G_wGCw'C
Cuts at: 0 940 1105 1196 1231 1577 2214
Size: 940 165 91 35 346 637
Fragments arranged by size:
940 637 346 165 91 35
BsmI GAATG_Cn'
Cuts at: 0 583 1782 2214
Size: 583 1199 432
Fragments arranged by size:
1199 583 432
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1720 2184 2214
Size: 1720 464 30
Fragments arranged by size:
1720 464 30
Bsp1286I G_dGCh'C
Cuts at: 0 57 272 383 940 1105 1196 1231 1415
Size: 57 215 111 557 165 91 35 184
Cuts at: 1415 1577 2214
Size: 162 637
Fragments arranged by size:
637 557 215 184 165 162 111 91
57 35
BspEI T'CCGG_A
Cuts at: 0 1223 2214
Size: 1223 991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1849 2214
Size: 1849 365
BsrDI GCAATG_nn'
Cuts at: 0 143 506 591 1665 2214
Size: 143 363 85 1074 549
Fragments arranged by size:
1074 549 363 143 85
BsrFI r'CCGG_y
Cuts at: 0 2149 2214
Size: 2149 65
BssSI C'ACGA_G
Cuts at: 0 2101 2214
Size: 2101 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 64 1392 1975 2214
Size: 64 1328 583 239
Fragments arranged by size:
1328 583 239 64
BstYI r'GATC_y
Cuts at: 0 1684 2214
Size: 1684 530
BtgI C'CryG_G
Cuts at: 0 63 120 1680 2214
Size: 63 57 1560 534
Fragments arranged by size:
1560 534 63 57
EaeI y'GGCC_r
Cuts at: 0 156 172 917 1394 1991 2147 2151 2214
Size: 156 16 745 477 597 156 4 63
Fragments arranged by size:
745 597 477 156 156 63 16 4
EarI CTCTTCn'nnn_
Cuts at: 0 1013 2202 2214
Size: 1013 1189 12
Fragments arranged by size:
1189 1013 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2172 2214
Size: 2172 42
EcoICRI GAG'CTC
Cuts at: 0 1103 1194 1575 2214
Size: 1103 91 381 639
Fragments arranged by size:
1103 639 381 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 813 1037 1089 1115 1513 1787 2034
Size: 131 682 224 52 26 398 274 247
Cuts at: 2034 2214
Size: 180
Fragments arranged by size:
682 398 274 247 224 180 131 52
26
EcoO109I rG'GnC_Cy
Cuts at: 0 200 883 1488 1971 2214
Size: 200 683 605 483 243
Fragments arranged by size:
683 605 483 243 200
EcoRI G'AATT_C
Cuts at: 0 1468 2214
Size: 1468 746
EcoRV GAT'ATC
Cuts at: 0 1707 2214
Size: 1707 507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1578 2214
Size: 1578 636
FseI GG_CCGG'CC
Cuts at: 0 2153 2214
Size: 2153 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 824 856 1012 1044 1092 1124
Size: 19 32 773 32 156 32 48 32
Cuts at: 1124 2185 2214
Size: 1061 29
Fragments arranged by size:
1061 773 156 48 32 32 32 32
29 19
HincII GTy'rAC
Cuts at: 0 573 1083 2214
Size: 573 510 1131
Fragments arranged by size:
1131 573 510
HindIII A'AGCT_T
Cuts at: 0 1655 2214
Size: 1655 559
HpaI GTT'AAC
Cuts at: 0 1083 2214
Size: 1083 1131
KpnI G_GTAC'C
Cuts at: 0 306 1425 2214
Size: 306 1119 789
Fragments arranged by size:
1119 789 306
MfeI C'AATT_G
Cuts at: 0 836 2214
Size: 836 1378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1301 2183 2214
Size: 1301 882 31
Fragments arranged by size:
1301 882 31
MscI TGG'CCA
Cuts at: 0 158 174 919 1396 1993 2214
Size: 158 16 745 477 597 221
Fragments arranged by size:
745 597 477 221 158 16
MslI CAynn'nnrTG
Cuts at: 0 62 925 1439 2214
Size: 62 863 514 775
Fragments arranged by size:
863 775 514 62
MspA1I CmG'CkG
Cuts at: 0 50 987 1189 1517 1875 2214
Size: 50 937 202 328 358 339
Fragments arranged by size:
937 358 339 328 202 50
NaeI GCC'GGC
Cuts at: 0 2151 2214
Size: 2151 63
NcoI C'CATG_G
Cuts at: 0 63 120 1680 2214
Size: 63 57 1560 534
Fragments arranged by size:
1560 534 63 57
NgoMIV G'CCGG_C
Cuts at: 0 2149 2214
Size: 2149 65
PasI CC'CwG_GG
Cuts at: 0 307 2214
Size: 307 1907
PflMI CCAn_nnn'nTGG
Cuts at: 0 915 2145 2214
Size: 915 1230 69
Fragments arranged by size:
1230 915 69
PfoI T'CCnGG_A
Cuts at: 0 221 1850 2214
Size: 221 1629 364
Fragments arranged by size:
1629 364 221
PpuMI rG'GwC_Cy
Cuts at: 0 200 883 2214
Size: 200 683 1331
Fragments arranged by size:
1331 683 200
PspOMI G'GGCC_C
Cuts at: 0 53 2214
Size: 53 2161
PstI C_TGCA'G
Cuts at: 0 986 2071 2214
Size: 986 1085 143
Fragments arranged by size:
1085 986 143
PvuII CAG'CTG
Cuts at: 0 50 987 1189 2214
Size: 50 937 202 1025
Fragments arranged by size:
1025 937 202 50
SacI G_AGCT'C
Cuts at: 0 1105 1196 1577 2214
Size: 1105 91 381 637
Fragments arranged by size:
1105 637 381 91
SanDI GG'GwC_CC
Cuts at: 0 200 2214
Size: 200 2014
ScaI AGT'ACT
Cuts at: 0 1741 2214
Size: 1741 473
SfcI C'TryA_G
Cuts at: 0 147 372 982 1446 2067 2214
Size: 147 225 610 464 621 147
Fragments arranged by size:
621 610 464 225 147 147
SmlI C'TyrA_G
Cuts at: 0 1735 2214
Size: 1735 479
StuI AGG'CCT
Cuts at: 0 498 2214
Size: 498 1716
StyI C'CwwG_G
Cuts at: 0 63 120 1680 1974 2214
Size: 63 57 1560 294 240
Fragments arranged by size:
1560 294 240 63 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1837 2214
Size: 1837 377
TatI w'GTAC_w
Cuts at: 0 1739 2214
Size: 1739 475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 930 962 2214
Size: 930 32 1252
Fragments arranged by size:
1252 930 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 912 2161 2214
Size: 912 1249 53
Fragments arranged by size:
1249 912 53
XhoI C'TCGA_G
Cuts at: 0 1735 2214
Size: 1735 479
Enzymes that do cut:
Acc65I AcuI AhdI ApaI ApoI AvaI BanI BanII
BbsI BcgI BciVI BlpI Bme1580I BmrI BpmI BsaI
BsaBI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrFI BssSI BstXI BstYI BtgI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI Hin4I HincII HindIII HpaI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
ScaI SfcI SmlI StuI StyI TaqII TatI TstI
XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaHI
BsaXI BsgI BsiEI BsiWI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoNI FspI FspAI
HaeII KasI MluI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI
XmnI ZraI