Restriction map for
pFN21AB3122
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstUI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT14I
EcoT22I FbaI Fnu4HI FokI FseI
FspI HapII HgaI Hin1I HpaI
HpaII ItaI KasI KpnI KspI
MaeI MaeII MamI MflI MluI
MroI MspA1I MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI TaqI
TseI TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 332 GTAGAC GT^[AC][GT]AC
AciI 505 CCGC CCGC(-3/-1)
AcsI 118 GAATTC [GA]^AATT[CT]
AfaI 125 GTAC GT^AC
AlwI 499 GATCC GGATC(4/5)
AlwNI 341 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 118 GAATTC [GA]^AATT[CT]
AspHI 183 GAGCAC G[AT]GC[AT]^C
AspI 592 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 528 TGGCCA TGG^CCA
BanI 630 GGTGCC G^G[CT][GA]CC
BbvCI 367 CCTCAGC CCTCAGC(-5/-2)
BmyI 183 GAGCAC G[AGT]GC[ACT]^C
BsaJI 219 CCGAGG C^C[ACGT][ACGT]GG
BseRI 546 GAGGAG GAGGAG(10/8)
BsiHKAI 183 GAGCAC G[AT]GC[AT]^C
BsmBI 474 CGTCTC CGTCTC(1/5)
Bsp1286I 183 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 124 TGTACA T^GTACA
BspMI 539 ACCTGC ACCTGC(4/8)
BsrGI 124 TGTACA T^GTACA
BssKI 436 CCAGG ^CC[ACGT]GG
BstEII 639 GGTCACC G^GT[ACGT]ACC
BstNI 436 CCAGG CC^[AT]GG
BstPI 639 GGTCACC G^GT[ACGT]ACC
BstXI 620 CCATTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 499 GATC ^GATC
EaeI 528 TGGCCA [CT]^GGCC[GA]
EarI 271 CTCTTC CTCTTC(1/4)
EcoO65I 639 GGTCACC G^GT[ACGT]ACC
EcoRI 118 GAATTC G^AATTC
EcoRII 436 CCAGG ^CC[AT]GG
FauI 504 CCCGC CCCGC(4/6)
HaeII 461 GGCGCT [GA]GCGC^[CT]
HincII 598 GTCAAC GT[CT]^[GA]AC
HindII 598 GTCAAC GT[CT]^[GA]AC
HindIII 146 AAGCTT A^AGCTT
HinfI 469 GATTC G^A[ACGT]TC
Ksp632I 271 CTCTTC CTCTTC(1/4)
MboI 499 GATC ^GATC
MfeI 492 CAATTG C^AATTG
MluNI 528 TGGCCA TGG^CCA
MscI 528 TGGCCA TGG^CCA
MslI 621 CATTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 492 CAATTG C^AATTG
MvaI 436 CCAGG CC^[AT]GG
MwoI 139 GCAAGAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 499 GATC ^GATC
NlaIV 630 GGTGCC GG[ACGT]^[ACGT]CC
PflFI 592 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 541 CTGCAG CTGCA^G
RsaI 125 GTAC GT^AC
Sau3AI 499 GATC ^GATC
ScrFI 436 CCAGG CC^[ACGT]GG
SfaNI 308 GATGC GCATC(5/9)
SfcI 541 CTGCAG C^T[GA][CT]AG
SspBI 124 TGTACA T^GTACA
SspI 379 AATATT AAT^ATT
TfiI 469 GATTC G^A[AT]TC
Tth111I 592 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 7 enzymes) #
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AluI 60 AGCT AG^CT
147 AGCT
BpmI 434 CTCCAG CTGGAG(16/14)
303 CTGGAG
BsmAI 432 GTCTC GTCTC(1/5)
475 GTCTC
CfoI 199 GCGC GCG^C
462 GCGC
HhaI 199 GCGC GCG^C
462 GCGC
HinP1I 199 GCGC G^CGC
462 GCGC
HphI 386 TCACC GGTGA(8/7)
641 TCACC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BsrI DdeI HaeIII MaeIII MboII
MnlI MseI Tru9I Tsp45I Tsp509I
TspRI