Restriction map for
pFN21AB3122
(Circular) (Six-base) MAPSORT of: pFN21AB3122.seq Check: 765 from: 1 to: 5359
pFN21AB3122 5359 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3169 278
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2338 2702 1937
Size: 401 364 4594
Fragments arranged by size:
4594 401 364
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2373 3422 4806 5074 1632
Size: 741 1049 1384 268 1917
Fragments arranged by size:
1917 1384 1049 741 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 4258 5235 1203
Size: 3055 977 1327
Fragments arranged by size:
3055 1327 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5359
AlwNI CAG_nnn'CTG
Cuts at: 2351 3102 4674 2351
Size: 751 1572 3036
Fragments arranged by size:
3036 1572 751
ApaLI G'TGCA_C
Cuts at: 3416 4572 3416
Size: 1156 4203
ApoI r'AATT_y
Cuts at: 1493 2123 2668 2894 3046 5155 1493
Size: 630 545 226 152 2109 1697
Fragments arranged by size:
2109 1697 630 545 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 1870
Size: 73 741 4545
Fragments arranged by size:
4545 741 73
BamHI G'GATC_C
Cuts at: 2689 3056 2689
Size: 367 4992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 2680 4135 618
Size: 325 217 627 848 45 1455 1842
Fragments arranged by size:
1842 1455 848 627 325 217 45
BanII G_rGCy'C
Cuts at: 1391 2678 1391
Size: 1287 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4388
BbvCI CC'TCA_GC
Cuts at: 2373 2373
Size: 5359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3970 136
Size: 107 122 71 631 605 2298 1525
Fragments arranged by size:
2298 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5354 5354
Size: 5359
BlpI GC'TnA_GC
Cuts at: 2776 2776
Size: 5359
Bme1580I G_kGCm'C
Cuts at: 1790 3420 4576 1790
Size: 1630 1156 2573
Fragments arranged by size:
2573 1630 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3090 4048 442
Size: 416 53 83 399 24 1673 958 1753
Fragments arranged by size:
1753 1673 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2329 2424 4019 1239
Size: 1090 95 1595 2579
Fragments arranged by size:
2579 1595 1090 95
Bpu10I CC'TnA_GC
Cuts at: 2373 2373
Size: 5359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3484 4349 4647 3484
Size: 865 298 4196
Fragments arranged by size:
4196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4022 882
Size: 617 2523 2219
Fragments arranged by size:
2523 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 493
Size: 711 1946 2702
Fragments arranged by size:
2702 1946 711
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3166 3548 4183 275
Size: 53 83 186 936 1633 382 635 1451
Fragments arranged by size:
1633 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3792 4190 4464 4611 5022 3792
Size: 398 274 147 411 4129
Fragments arranged by size:
4129 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5134
Fragments arranged by size:
5134 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2566 1186
Size: 1380 3979
BseYI C'CCAG_C
Cuts at: 4562 5058 4562
Size: 496 4863
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4786
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2809 3570 4598 1540
Size: 275 186 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2192 3420 3505 4576 2192
Size: 1228 85 1071 2975
Fragments arranged by size:
2975 1228 1071 85
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
BsmBI CGTCTCn'nnnn_
Cuts at: 2485 2485
Size: 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2678 3420 3505 4576 1391
Size: 399 402 486 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 402 399 85
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2553 2714 844
Size: 1709 161 3489
Fragments arranged by size:
3489 1709 161
BsrBI CCG'CTC
Cuts at: 1394 1823 3246 1394
Size: 429 1423 3507
Fragments arranged by size:
3507 1423 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4003 4190 1700
Size: 110 2193 187 2869
Fragments arranged by size:
2869 2193 187 110
BsrGI T'GTAC_A
Cuts at: 96 2129 96
Size: 2033 3326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5359
BssSI C'ACGA_G
Cuts at: 3419 4431 3419
Size: 1012 4347
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5359
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 2644 1184
Size: 1460 3899
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 2632
Size: 5359
BstYI r'GATC_y
Cuts at: 1972 2689 3056 3441 3458 4910 4996 5008 5354
Size: 717 367 385 17 1452 86 12 346
Cuts at: 5354 1972
Size: 1977
Fragments arranged by size:
1977 1452 717 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3868
Fragments arranged by size:
3868 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 2875 3669 3689 2434
Size: 441 794 20 4104
Fragments arranged by size:
4104 794 441 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5324
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 2806 3695
Size: 54 1425 50 275 18 703 273 889
Cuts at: 3695 5324 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 703 275 273 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2806 1537
Size: 275 994 4090
Fragments arranged by size:
4090 994 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 3289 1353
Size: 929 1007 3423
Fragments arranged by size:
3423 1007 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3946 4320 4466 387
Size: 1064 138 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2329 2373 2424 3422 4019 4806 5074
Size: 393 697 44 51 998 597 787 268
Cuts at: 5074 1239
Size: 1524
Fragments arranged by size:
1524 998 787 697 597 393 268 51
44
EcoRI G'AATT_C
Cuts at: 2123 2668 3046 2123
Size: 545 378 4436
Fragments arranged by size:
4436 545 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2169 2169
Size: 5359
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 2470 4506 1678
Size: 792 2036 2531
Fragments arranged by size:
2531 2036 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 4000 4032 4074
Size: 32 135 32 893 32 1503 32 42
Cuts at: 4074 4106 1373
Size: 32 2626
Fragments arranged by size:
2626 1503 893 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2605 2703 2949 669
Size: 988 281 667 98 246 3079
Fragments arranged by size:
3079 988 667 281 246 98
HindIII A'AGCT_T
Cuts at: 748 2151 748
Size: 1403 3956
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2497 2958 2497
Size: 461 4898
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 4473 4657 1305
Size: 1329 1839 184 2007
Fragments arranged by size:
2007 1839 1329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2535 10
Size: 54 1425 343 703 2834
Fragments arranged by size:
2834 1425 703 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3317 3676 3835 518
Size: 648 1464 687 359 159 2042
Fragments arranged by size:
2042 1464 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2775 3452 4600 4845 1606
Size: 1169 677 1148 245 2120
Fragments arranged by size:
2120 1169 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3868
Fragments arranged by size:
3868 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2717 4262 1132
Size: 97 1488 1545 2229
Fragments arranged by size:
2229 1545 1488 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5359
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5359
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2550 2711 830
Size: 1720 161 3478
Fragments arranged by size:
3478 1720 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2546 2707 3842 4523 4714 826
Size: 219 1501 161 1135 681 191 1471
Fragments arranged by size:
1501 1471 1135 681 219 191 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5359
SmaI CCC'GGG
Cuts at: 1872 2686 1872
Size: 814 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3463 4364 4626 820
Size: 197 926 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 2386 3283 5
Size: 47 2334 897 2081
Fragments arranged by size:
2334 2081 897 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5359
StyI C'CwwG_G
Cuts at: 513 1065 2004 2798 513
Size: 552 939 794 3074
Fragments arranged by size:
3074 939 794 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3403 3556 3741 4186 325
Size: 1255 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2129 3605 5317
Size: 274 80 33 51 1443 152 1476 1712
Cuts at: 5317 96
Size: 138
Fragments arranged by size:
1712 1476 1443 274 152 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5319 5351 5319
Size: 32 5327
Tth111I GACn'n_nGTC
Cuts at: 1316 2600 1316
Size: 1284 4075
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2684 1870
Size: 814 4545
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3167 276
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI