(Circular) (Six-base) MAPSORT of: pFN21AB3115.seq Check: 9191 from: 1 to: 5974 pFN21AB3115 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3167 4037 5421 5689 1632 Size: 977 558 870 1384 268 1917 Fragments arranged by size: 1917 1384 977 870 558 268 AflII C'TTAA_G Cuts at: 820 1017 2375 820 Size: 197 1358 4419 Fragments arranged by size: 4419 1358 197 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2356 3717 5289 2356 Size: 1361 1572 3041 Fragments arranged by size: 3041 1572 1361 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 3283 3509 3661 5770 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2141 3299 1870 Size: 73 198 1158 4545 Fragments arranged by size: 4545 1158 198 73 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2149 2608 3233 3293 1391 Size: 758 459 625 60 4072 Fragments arranged by size: 4072 758 625 459 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2085 3133 1548 Size: 537 1048 4389 Fragments arranged by size: 4389 1048 537 BfrBI ATG'CAT Cuts at: 2780 2780 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 4035 5191 1790 Size: 2245 1156 2573 Fragments arranged by size: 2573 2245 1156 BmgBI CAC'GTC Cuts at: 1318 2494 1318 Size: 1176 4798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2205 2592 3705 Size: 416 53 83 399 24 788 387 1113 Cuts at: 3705 4663 442 Size: 958 1753 Fragments arranged by size: 1753 1113 958 788 416 399 387 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2459 1056 Size: 1403 4571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 4634 1239 Size: 800 2595 2579 Fragments arranged by size: 2595 2579 800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2040 4099 4964 5262 2040 Size: 2059 865 298 2752 Fragments arranged by size: 2752 2059 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 2507 5968 2507 Size: 3461 2513 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2175 3781 4163 Size: 53 83 186 936 476 166 1606 382 Cuts at: 4163 4798 275 Size: 635 1451 Fragments arranged by size: 1606 1451 936 635 476 382 186 166 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5974 BseYI C'CCAG_C Cuts at: 2306 3112 5177 5673 2306 Size: 806 2065 496 2607 Fragments arranged by size: 2607 2065 806 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3002 3005 1163 Size: 573 1266 3 4132 Fragments arranged by size: 4132 1266 573 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 2280 2608 4035 4120 5191 2142 Size: 138 328 1427 85 1071 2925 Fragments arranged by size: 2925 1427 1071 328 138 85 BsmI GAATG_Cn' Cuts at: 3485 3578 3485 Size: 93 5881 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2149 2280 2608 3233 3293 4035 Size: 399 352 7 131 328 625 60 742 Cuts at: 4035 4120 5191 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 625 399 352 328 131 85 60 7 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3169 3861 1394 Size: 429 1346 692 3507 Fragments arranged by size: 3507 1346 692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2782 2900 4469 4643 65 Size: 1183 1534 118 1569 174 1396 Fragments arranged by size: 1569 1534 1396 1183 174 118 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 2155 3214 96 Size: 2059 1059 2856 Fragments arranged by size: 2856 2059 1059 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 2786 4034 5046 2786 Size: 1248 1012 3714 Fragments arranged by size: 3714 1248 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 2226 4778 2226 Size: 2552 3422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstYI r'GATC_y Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969 Size: 1332 367 385 17 1452 86 12 346 Cuts at: 5969 1972 Size: 1977 Fragments arranged by size: 1977 1452 1332 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2997 2997 Size: 5974 BtgI C'CryG_G Cuts at: 513 1065 2004 2643 3180 513 Size: 552 939 639 537 3307 Fragments arranged by size: 3307 939 639 552 537 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 3490 4284 4304 3490 Size: 794 20 5160 Fragments arranged by size: 5160 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2077 2112 529 Size: 35 1513 35 4391 Fragments arranged by size: 4391 1513 35 35 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3074 4981 809 Size: 507 1758 1907 1802 Fragments arranged by size: 1907 1802 1758 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939 Size: 54 1425 50 275 18 1591 889 1629 Cuts at: 5939 8 Size: 43 Fragments arranged by size: 1629 1591 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 3904 1353 Size: 905 1646 3423 Fragments arranged by size: 3423 1646 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2606 2606 Size: 5974 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2609 3167 4037 4634 5421 5689 Size: 393 407 570 558 870 597 787 268 Cuts at: 5689 1239 Size: 1524 Fragments arranged by size: 1524 870 787 597 570 558 407 393 268 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 EcoRV GAT'ATC Cuts at: 2509 2509 Size: 5974 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2914 2914 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2559 2591 4615 4647 4689 Size: 32 135 32 987 32 2024 32 42 Cuts at: 4689 4721 1373 Size: 32 2626 Fragments arranged by size: 2626 2024 987 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2065 2646 5088 5272 1305 Size: 760 581 2442 184 2007 Fragments arranged by size: 2442 2007 760 581 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3932 4291 4450 518 Size: 648 2766 359 159 2042 Fragments arranged by size: 2766 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2416 3390 4067 5215 5460 1606 Size: 810 974 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 810 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2643 3180 513 Size: 552 939 639 537 3307 Fragments arranged by size: 3307 939 639 552 537 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 2455 1052 Size: 1403 4571 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NruI TCG'CGA Cuts at: 2868 2868 Size: 5974 NsiI A_TGCA'T Cuts at: 2782 2782 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2432 3332 4877 1132 Size: 97 1203 900 1545 2229 Fragments arranged by size: 2229 1545 1203 900 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 2913 3544 2913 Size: 631 5343 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2416 2416 Size: 5974 SacI G_AGCT'C Cuts at: 2608 2608 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SapI GCTCTTCn'nnn_ Cuts at: 2258 2258 Size: 5974 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SexAI A'CCwGG_T Cuts at: 2835 2835 Size: 5974 SfcI C'TryA_G Cuts at: 826 1045 3322 4457 5138 5329 826 Size: 219 2277 1135 681 191 1471 Fragments arranged by size: 2277 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2055 2375 4078 4979 5241 820 Size: 197 926 112 320 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 320 262 197 112 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 2923 3898 5 Size: 47 2871 975 2081 Fragments arranged by size: 2871 2081 975 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 2643 3180 3413 513 Size: 552 939 165 474 537 233 3074 Fragments arranged by size: 3074 939 552 537 474 233 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4001 4018 4171 4356 4801 325 Size: 1255 2421 17 153 185 445 1498 Fragments arranged by size: 2421 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2155 2716 3214 Size: 274 80 33 51 1443 178 561 498 Cuts at: 3214 4220 5932 96 Size: 1006 1712 138 Fragments arranged by size: 1712 1443 1006 561 498 274 178 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5934 5966 5934 Size: 32 5942 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI