Restriction map for
pFN21AE0981
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AflIII AhdI AlwI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BlnI BpuAI BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BstAPI BstBI BstEII BstPI
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HinfI HpaI
KpnI MamI MluI MroI MseI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PleI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 1093 AACGTT AA^CGTT
AgeI 9 ACCGGT A^CCGGT
ApaI 518 GGGCCC GGGCC^C
AvaI 481 CCCGAG C^[CT]CG[GA]G
AviII 1442 TGCGCA TGC^GCA
BalI 1649 TGGCCA TGG^CCA
BfaI 508 CTAG C^TAG
BglII 287 AGATCT A^GATCT
BlpI 1042 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1042 GCTGAGC GC^T[ACGT]AGC
BsaWI 9 ACCGGT [AT]^CCGG[AT]
BsiEI 7 CGACCG CG[GA][CT]^CG
BsoBI 481 CCCGAG C^[CT]CG[GA]G
BssHII 1461 GCGCGC G^CGCGC
Bst1107I 486 GTATAC GTA^TAC
BstXI 1427 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 287 AGATCT [GA]^GATC[CT]
BstZ17I 486 GTATAC GTA^TAC
CelII 1042 GCTGAGC GC^T[ACGT]AGC
DpnII 288 GATC ^GATC
FspI 1442 TGCGCA TGC^GCA
HgaI 1225 GACGC GACGC(5/10)
HincII 967 GTCAAC GT[CT]^[GA]AC
HindII 967 GTCAAC GT[CT]^[GA]AC
MaeI 508 CTAG C^TAG
MaeIII 377 GTCAC ^GT[ACGT]AC
MboI 288 GATC ^GATC
MfeI 614 CAATTG C^AATTG
MflI 287 AGATCT [GA]^GATC[CT]
MluNI 1649 TGGCCA TGG^CCA
MscI 1649 TGGCCA TGG^CCA
MunI 614 CAATTG C^AATTG
NaeI 955 GCCGGC GCC^GGC
NdeII 288 GATC ^GATC
NgoMIV 955 GCCGGC G^CCGGC
PinAI 9 ACCGGT A^CCGGT
Psp1406I 1093 AACGTT AA^CGTT
PstI 283 CTGCAG CTGCA^G
SapI 588 GCTCTTC GCTCTTC(1/4)
Sau3AI 288 GATC ^GATC
SfcI 283 CTGCAG C^T[GA][CT]AG
StuI 1034 AGGCCT AGG^CCT
Tsp45I 377 GTCAC ^GT[GC]AC
Tsp509I 615 AATT ^AATT
XcmI 155 CCACCGCGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 287 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AccI 486 GTATAC GT^[AC][GT]AC
1567 GTCTAC
AfaI 690 GTAC GT^AC
1728 GTAC
Aor51HI 857 AGCGCT AGC^GCT
1382 AGCGCT
BsaAI 433 TACGTG [CT]AC^GT[GA]
1554 CACGTA
EaeI 64 TGGCCG [CT]^GGCC[GA]
1649 TGGCCA
Eco47III 857 AGCGCT AGC^GCT
1382 AGCGCT
EcoNI 630 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
813 CCTGGCCCAGG
HphI 363 GGTGA GGTGA(8/7)
378 TCACC
KspI 158 CCGCGG CCGC^GG
1420 CCGCGG
RsaI 690 GTAC GT^AC
1728 GTAC
SacII 158 CCGCGG CCGC^GG
1420 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AluI Alw44I
AlwNI ApaLI AspHI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI Ksp632I
MaeII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV PflMI PpuMI
PvuII SacI Sau96I ScrFI SfaNI
SfoI SmlI StyI TaqI TseI
TspRI TthHB8I Van91I