Restriction map for
pFN21AE0981
(Linear) (Six-base) MAPSORT of: KIEE0981.seq Check: 4792 from: 1 to: 1740
KIEE0981.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 479 1740
Size: 479 1261
AccI GT'mk_AC
Cuts at: 0 487 1568 1740
Size: 487 1081 172
Fragments arranged by size:
1081 487 172
AclI AA'CG_TT
Cuts at: 0 1094 1740
Size: 1094 646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 292 765 823 1740
Size: 69 223 473 58 917
Fragments arranged by size:
917 473 223 69 58
AfeI AGC'GCT
Cuts at: 0 859 1384 1740
Size: 859 525 356
Fragments arranged by size:
859 525 356
AgeI A'CCGG_T
Cuts at: 0 9 1740
Size: 9 1731
AleI CACnn'nnGTG
Cuts at: 0 135 670 1740
Size: 135 535 1070
Fragments arranged by size:
1070 535 135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 949 1206 1740
Size: 949 257 534
Fragments arranged by size:
949 534 257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 1740
Size: 538 32 1170
Fragments arranged by size:
1170 538 32
AlwNI CAG_nnn'CTG
Cuts at: 0 453 801 825 999 1164 1731 1740
Size: 453 348 24 174 165 567 9
Fragments arranged by size:
567 453 348 174 165 24 9
ApaI G_GGCC'C
Cuts at: 0 522 1740
Size: 522 1218
ApaLI G'TGCA_C
Cuts at: 0 309 640 907 1740
Size: 309 331 267 833
Fragments arranged by size:
833 331 309 267
AvaI C'yCGr_G
Cuts at: 0 481 1740
Size: 481 1259
BanI G'GyrC_C
Cuts at: 0 171 664 897 1483 1549 1573 1740
Size: 171 493 233 586 66 24 167
Fragments arranged by size:
586 493 233 171 167 66 24
BanII G_rGCy'C
Cuts at: 0 192 522 590 740 1154 1330 1721 1740
Size: 192 330 68 150 414 176 391 19
Fragments arranged by size:
414 391 330 192 176 150 68 19
BbeI G_GCGC'C
Cuts at: 0 175 1487 1553 1740
Size: 175 1312 66 187
Fragments arranged by size:
1312 187 175 66
BglI GCCn_nnn'nGGC
Cuts at: 0 403 631 1237 1399 1547 1672 1740
Size: 403 228 606 162 148 125 68
Fragments arranged by size:
606 403 228 162 148 125 68
BglII A'GATC_T
Cuts at: 0 287 1740
Size: 287 1453
BlpI GC'TnA_GC
Cuts at: 0 1043 1740
Size: 1043 697
Bme1580I G_kGCm'C
Cuts at: 0 313 522 644 902 911 1706 1740
Size: 313 209 122 258 9 795 34
Fragments arranged by size:
795 313 258 209 122 34 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 921 1264 1740
Size: 921 343 476
Fragments arranged by size:
921 476 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 754 778 1186 1554 1660 1740
Size: 105 649 24 408 368 106 80
Fragments arranged by size:
649 408 368 106 105 80 24
Bpu10I CC'TnA_GC
Cuts at: 0 1408 1633 1693 1740
Size: 1408 225 60 47
Fragments arranged by size:
1408 225 60 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 580 1600 1740
Size: 508 72 1020 140
Fragments arranged by size:
1020 508 140 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 272 1636 1729 1740
Size: 272 1364 93 11
Fragments arranged by size:
1364 272 93 11
BsaAI yAC'GTr
Cuts at: 0 435 1556 1740
Size: 435 1121 184
Fragments arranged by size:
1121 435 184
BsaHI Gr'CG_yC
Cuts at: 0 172 1226 1484 1550 1740
Size: 172 1054 258 66 190
Fragments arranged by size:
1054 258 190 172 66
BsaWI w'CCGG_w
Cuts at: 0 9 1740
Size: 9 1731
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 1740
Size: 352 30 1358
Fragments arranged by size:
1358 352 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 237 1183 1729 1740
Size: 172 65 946 546 11
Fragments arranged by size:
946 546 172 65 11
BseYI C'CCAG_C
Cuts at: 0 441 769 785 1707 1740
Size: 441 328 16 922 33
Fragments arranged by size:
922 441 328 33 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 34 640 706 1740
Size: 28 6 606 66 1034
Fragments arranged by size:
1034 606 66 28 6
BsiEI CG_ry'CG
Cuts at: 0 10 1740
Size: 10 1730
BsiHKAI G_wGCw'C
Cuts at: 0 152 313 460 590 644 740 911 938
Size: 152 161 147 130 54 96 171 27
Cuts at: 938 1409 1683 1721 1740
Size: 471 274 38 19
Fragments arranged by size:
471 274 171 161 152 147 130 96
54 38 27 19
Bsp1286I G_dGCh'C
Cuts at: 0 152 192 313 460 522 590 644 740
Size: 152 40 121 147 62 68 54 96
Cuts at: 740 902 911 938 1154 1330 1409 1683 1706
Size: 162 9 27 216 176 79 274 23
Cuts at: 1706 1721 1740
Size: 15 19
Fragments arranged by size:
274 216 176 162 152 147 121 96
79 68 62 54 40 27 23 19
15 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 479 707 1740
Size: 254 225 228 1033
Fragments arranged by size:
1033 254 228 225
BsrFI r'CCGG_y
Cuts at: 0 9 955 1060 1740
Size: 9 946 105 680
Fragments arranged by size:
946 680 105 9
BssHII G'CGCG_C
Cuts at: 0 1461 1740
Size: 1461 279
BssSI C'ACGA_G
Cuts at: 0 235 583 1676 1699 1740
Size: 235 348 1093 23 41
Fragments arranged by size:
1093 348 235 41 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1434 1740
Size: 1434 306
BstYI r'GATC_y
Cuts at: 0 287 1740
Size: 287 1453
BstZ17I GTA'TAC
Cuts at: 0 488 1740
Size: 488 1252
BtgI C'CryG_G
Cuts at: 0 158 1146 1420 1490 1740
Size: 158 988 274 70 250
Fragments arranged by size:
988 274 250 158 70
BtsI GCAGTG_nn'
Cuts at: 0 938 1010 1740
Size: 938 72 730
Fragments arranged by size:
938 730 72
EaeI y'GGCC_r
Cuts at: 0 64 1649 1740
Size: 64 1585 91
Fragments arranged by size:
1585 91 64
EarI CTCTTCn'nnn_
Cuts at: 0 214 332 595 1343 1740
Size: 214 118 263 748 397
Fragments arranged by size:
748 397 263 214 118
EcoICRI GAG'CTC
Cuts at: 0 588 738 1719 1740
Size: 588 150 981 21
Fragments arranged by size:
981 588 150 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 105 292 754 765 778 823 1186
Size: 69 36 187 462 11 13 45 363
Cuts at: 1186 1554 1660 1740
Size: 368 106 80
Fragments arranged by size:
462 368 363 187 106 80 69 45
36 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 634 817 1740
Size: 634 183 923
Fragments arranged by size:
923 634 183
EcoO109I rG'GnC_Cy
Cuts at: 0 102 426 518 519 573 789 798 809
Size: 102 324 92 1 54 216 9 11
Cuts at: 809 1740
Size: 931
Fragments arranged by size:
931 324 216 102 92 54 11 9
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 549 1740
Size: 549 1191
FspI TGC'GCA
Cuts at: 0 1444 1740
Size: 1444 296
HaeII r_GCGC'y
Cuts at: 0 175 258 861 1302 1386 1487 1553 1633
Size: 175 83 603 441 84 101 66 80
Cuts at: 1633 1740
Size: 107
Fragments arranged by size:
603 441 175 107 101 84 83 80
66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 1740
Size: 352 32 1356
Fragments arranged by size:
1356 352 32
HincII GTy'rAC
Cuts at: 0 969 1740
Size: 969 771
KasI G'GCGC_C
Cuts at: 0 171 1483 1549 1740
Size: 171 1312 66 191
Fragments arranged by size:
1312 191 171 66
MfeI C'AATT_G
Cuts at: 0 614 1740
Size: 614 1126
MscI TGG'CCA
Cuts at: 0 1651 1740
Size: 1651 89
MslI CAynn'nnrTG
Cuts at: 0 135 670 1402 1740
Size: 135 535 732 338
Fragments arranged by size:
732 535 338 135
MspA1I CmG'CkG
Cuts at: 0 160 384 785 1197 1320 1422 1711 1740
Size: 160 224 401 412 123 102 289 29
Fragments arranged by size:
412 401 289 224 160 123 102 29
NaeI GCC'GGC
Cuts at: 0 957 1740
Size: 957 783
NarI GG'CG_CC
Cuts at: 0 172 1484 1550 1740
Size: 172 1312 66 190
Fragments arranged by size:
1312 190 172 66
NgoMIV G'CCGG_C
Cuts at: 0 955 1740
Size: 955 785
PflMI CCAn_nnn'nTGG
Cuts at: 0 543 732 825 999 1087 1164 1731 1740
Size: 543 189 93 174 88 77 567 9
Fragments arranged by size:
567 543 189 174 93 88 77 9
PfoI T'CCnGG_A
Cuts at: 0 536 593 794 1163 1740
Size: 536 57 201 369 577
Fragments arranged by size:
577 536 369 201 57
PpuMI rG'GwC_Cy
Cuts at: 0 789 798 809 1740
Size: 789 9 11 931
Fragments arranged by size:
931 789 11 9
PspOMI G'GGCC_C
Cuts at: 0 518 1740
Size: 518 1222
PstI C_TGCA'G
Cuts at: 0 287 1740
Size: 287 1453
PvuII CAG'CTG
Cuts at: 0 785 1197 1320 1740
Size: 785 412 123 420
Fragments arranged by size:
785 420 412 123
SacI G_AGCT'C
Cuts at: 0 590 740 1721 1740
Size: 590 150 981 19
Fragments arranged by size:
981 590 150 19
SacII CC_GC'GG
Cuts at: 0 161 1423 1740
Size: 161 1262 317
Fragments arranged by size:
1262 317 161
SanDI GG'GwC_CC
Cuts at: 0 809 1740
Size: 809 931
SapI GCTCTTCn'nnn_
Cuts at: 0 595 1740
Size: 595 1145
SfcI C'TryA_G
Cuts at: 0 283 1740
Size: 283 1457
SfoI GGC'GCC
Cuts at: 0 173 1485 1551 1740
Size: 173 1312 66 189
Fragments arranged by size:
1312 189 173 66
SmlI C'TyrA_G
Cuts at: 0 523 559 1615 1740
Size: 523 36 1056 125
Fragments arranged by size:
1056 523 125 36
StuI AGG'CCT
Cuts at: 0 1036 1740
Size: 1036 704
StyI C'CwwG_G
Cuts at: 0 112 1595 1646 1740
Size: 112 1483 51 94
Fragments arranged by size:
1483 112 94 51
TatI w'GTAC_w
Cuts at: 0 689 1740
Size: 689 1051
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 289 321 1740
Size: 289 32 1419
Fragments arranged by size:
1419 289 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 1740
Size: 162 1578
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstXI
BstYI BstZ17I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI
MfeI MscI MslI MspA1I NaeI NarI NgoMIV PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SapI SfcI SfoI SmlI StuI StyI TatI TstI
XcmI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AhdI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BmgBI BmtI BplI BsaBI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI
BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FseI FspAI HindIII HpaI
KpnI MluI MmeI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI