Restriction map for
pFN21AE0977
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BcgI BciVI BclI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MamI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 161 CGCG CG^CG
AccIII 768 TCCGGA T^CCGGA
AflII 256 CTTAAG C^TTAAG
BalI 115 TGGCCA TGG^CCA
BamHI 882 GGATCC G^GATCC
BanI 669 GGCACC G^G[CT][GA]CC
BbrPI 41 CACGTG CAC^GTG
BbsI 918 GAAGAC GAAGAC(2/6)
BbvCI 489 CCTCAGC CCTCAGC(-5/-2)
BfaI 424 CTAG C^TAG
BfrI 256 CTTAAG C^TTAAG
BglI 1525 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 918 GAAGAC GAAGAC(2/6)
BsaWI 768 TCCGGA [AT]^CCGG[AT]
BseAI 768 TCCGGA T^CCGGA
BspEI 768 TCCGGA T^CCGGA
BspHI 311 TCATGA T^CATGA
BssSI 1166 CACGAG CACGAG(-5/-1)
BstUI 161 CGCG CG^CG
CfoI 894 GCGC GCG^C
DraIII 823 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 644 CCGTGG C^C[GA][CT]GG
EcoRI 18 GAATTC G^AATTC
EcoRV 1108 GATATC GAT^ATC
EcoT14I 445 CCTTGG C^C[AT][AT]GG
HhaI 894 GCGC GCG^C
HinP1I 894 GCGC G^CGC
HincII 1195 GTCAAC GT[CT]^[GA]AC
HindII 1195 GTCAAC GT[CT]^[GA]AC
MaeI 424 CTAG C^TAG
MluI 160 ACGCGT A^CGCGT
MluNI 115 TGGCCA TGG^CCA
MroI 768 TCCGGA T^CCGGA
MscI 115 TGGCCA TGG^CCA
MslI 477 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1514 CAGCTG C[AC]G^C[GT]G
MvnI 161 CGCG CG^CG
PmaCI 41 CACGTG CAC^GTG
PmlI 41 CACGTG CAC^GTG
PvuII 1514 CAGCTG CAG^CTG
RcaI 311 TCATGA T^CATGA
SacI 131 GAGCTC GAGCT^C
SapI 955 GAAGAGC GCTCTTC(1/4)
SgrAI 1354 CGCCGGTG C[GA]^CCGG[CT]G
SphI 378 GCATGC GCATG^C
StuI 1683 AGGCCT AGG^CCT
StyI 445 CCTTGG C^C[AT][AT]GG
TfiI 1426 GAATC G^A[AT]TC
Tsp45I 1062 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflIII 42 ACGTGT A^C[GA][CT]GT
160 ACGCGT
ApaI 1300 GGGCCC GGGCC^C
1441 GGGCCC
AspHI 131 GAGCTC G[AT]GC[AT]^C
1208 GTGCTC
BsaAI 41 CACGTG [CT]AC^GT[GA]
1130 CACGTA
BseRI 80 GAGGAG GAGGAG(10/8)
1618 GAGGAG
BsiHKAI 131 GAGCTC G[AT]GC[AT]^C
1208 GTGCTC
BspMI 434 ACCTGC ACCTGC(4/8)
1250 ACCTGC
BsrFI 1160 ACCGGC [GA]^CCGG[CT]
1355 GCCGGT
Cfr10I 1160 ACCGGC [GA]^CCGG[CT]
1355 GCCGGT
HgaI 159 GACGC GACGC(5/10)
51 GCGTC
MfeI 23 CAATTG C^AATTG
373 CAATTG
MseI 257 TTAA T^TAA
1478 TTAA
MunI 23 CAATTG C^AATTG
373 CAATTG
NspI 290 ACATGC [GA]CATG^[CT]
378 GCATGC
PflMI 819 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1165 CCACGAGATGG
PleI 320 GACTC GAGTC(4/5)
998 GACTC
PpuMI 524 GGGACCT [GA]G^G[AT]CC[CT]
1217 AGGACCT
PstI 575 CTGCAG CTGCA^G
979 CTGCAG
TaqI 129 TCGA T^CGA
1095 TCGA
Tru9I 257 TTAA T^TAA
1478 TTAA
TthHB8I 129 TCGA T^CGA
1095 TCGA
Van91I 819 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1165 CCACGAGATGG
XcmI 992 CCAGGGGACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1554 CCAGGAAGAAAATGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TseI Tsp509I TspRI XhoII