(Circular) (Six-base) MAPSORT of: pFN21AE0977.seq Check: 3301 from: 1 to: 6418 pFN21AE0977 6418 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3761 1937 Size: 1824 4594 Acc65I G'GTAC_C Cuts at: 3739 3739 Size: 6418 AclI AA'CG_TT Cuts at: 1563 4545 4918 1563 Size: 2982 373 3063 Fragments arranged by size: 3063 2982 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2235 4481 5865 6133 1632 Size: 603 2246 1384 268 1917 Fragments arranged by size: 2246 1917 1384 603 268 AflII C'TTAA_G Cuts at: 820 1017 2261 820 Size: 197 1244 4977 Fragments arranged by size: 4977 1244 197 AflIII A'CryG_T Cuts at: 1203 2047 2165 5317 6294 1203 Size: 844 118 3152 977 1327 Fragments arranged by size: 3152 1327 977 844 118 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 5147 5147 Size: 6418 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3707 1184 Size: 2523 3895 AlwNI CAG_nnn'CTG Cuts at: 4161 5733 4161 Size: 1572 4846 ApaI G_GGCC'C Cuts at: 3309 3450 3309 Size: 141 6277 ApaLI G'TGCA_C Cuts at: 4475 5631 4475 Size: 1156 5262 ApoI r'AATT_y Cuts at: 1493 2023 2797 2937 3727 3953 4105 6214 1493 Size: 530 774 140 790 226 152 2109 1697 Fragments arranged by size: 2109 1697 790 774 530 226 152 140 AseI AT'TA_AT Cuts at: 160 1032 4972 160 Size: 872 3940 1606 Fragments arranged by size: 3940 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 3743 1870 Size: 73 1800 4545 Fragments arranged by size: 4545 1800 73 BamHI G'GATC_C Cuts at: 2887 3748 4115 2887 Size: 861 367 5190 Fragments arranged by size: 5190 861 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2674 3739 5194 618 Size: 325 217 627 887 1065 1455 1842 Fragments arranged by size: 1842 1455 1065 887 627 325 217 BanII G_rGCy'C Cuts at: 1391 2140 2700 3309 3342 3450 3737 1391 Size: 749 560 609 33 108 287 4072 Fragments arranged by size: 4072 749 609 560 287 108 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2930 928 Size: 971 1031 4416 Fragments arranged by size: 4416 1031 971 BbvCI CC'TCA_GC Cuts at: 2495 2495 Size: 6418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4609 4643 1903 Size: 34 2672 34 3678 Fragments arranged by size: 3678 2672 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4310 5520 4310 Size: 1210 5208 BclI T'GATC_A Cuts at: 1548 1548 Size: 6418 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3536 5029 136 Size: 107 122 71 631 605 1864 1493 1525 Fragments arranged by size: 1864 1525 1493 631 605 122 107 71 BglII A'GATC_T Cuts at: 6413 6413 Size: 6418 BlpI GC'TnA_GC Cuts at: 3835 3835 Size: 6418 Bme1580I G_kGCm'C Cuts at: 1790 3309 3450 4479 5635 1790 Size: 1519 141 1029 1156 2573 Fragments arranged by size: 2573 1519 1156 1029 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4149 5107 442 Size: 416 53 83 399 24 2732 958 1753 Fragments arranged by size: 2732 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2951 3542 3645 5078 1239 Size: 1712 591 103 1433 2579 Fragments arranged by size: 2579 1712 1433 591 103 Bpu10I CC'TnA_GC Cuts at: 2495 2495 Size: 6418 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3455 3510 4543 5408 5706 3455 Size: 55 1033 865 298 4167 Fragments arranged by size: 4167 1033 865 298 55 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5081 882 Size: 617 3582 2219 Fragments arranged by size: 3582 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2048 3137 4209 493 Size: 711 844 1089 1072 2702 Fragments arranged by size: 2702 1089 1072 844 711 BsaBI GATnn'nnATC Cuts at: 6412 6412 Size: 6418 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4225 4607 5242 275 Size: 53 83 186 936 2692 382 635 1451 Fragments arranged by size: 2692 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2773 4851 5249 5523 5670 6081 2773 Size: 2078 398 274 147 411 3110 Fragments arranged by size: 3110 2078 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6193 Fragments arranged by size: 6193 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2100 3638 1186 Size: 914 1538 3966 Fragments arranged by size: 3966 1538 914 BseYI C'CCAG_C Cuts at: 3260 5621 6117 3260 Size: 2361 496 3561 Fragments arranged by size: 3561 2361 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5845 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3868 4629 5657 1540 Size: 275 186 1867 761 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2140 3217 4479 4564 5635 2140 Size: 1077 1262 85 1071 2923 Fragments arranged by size: 2923 1262 1077 1071 85 BsmI GAATG_Cn' Cuts at: 3929 4022 3929 Size: 93 6325 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2700 3217 3309 3342 3450 3737 Size: 399 350 560 517 92 33 108 287 Cuts at: 3737 4479 4564 5635 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 560 517 399 350 287 108 92 85 33 BspEI T'CCGG_A Cuts at: 2773 6081 2773 Size: 3308 3110 BspHI T'CATG_A Cuts at: 2316 4307 6037 2316 Size: 1991 1730 2697 Fragments arranged by size: 2697 1991 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2448 3264 3773 844 Size: 1604 816 509 3489 Fragments arranged by size: 3489 1604 816 509 BsrBI CCG'CTC Cuts at: 1394 1823 4305 1394 Size: 429 2482 3507 Fragments arranged by size: 3507 2482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4913 5087 65 Size: 1183 3665 174 1396 Fragments arranged by size: 3665 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3165 3360 5062 5249 1700 Size: 110 1355 195 1702 187 2869 Fragments arranged by size: 2869 1702 1355 195 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 3171 4478 5490 3171 Size: 1307 1012 4099 Fragments arranged by size: 4099 1307 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6418 BstBI TT'CG_AA Cuts at: 5222 5222 Size: 6418 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstYI r'GATC_y Cuts at: 1972 2613 2887 3054 3489 3748 4115 4500 4517 Size: 641 274 167 435 259 367 385 17 Cuts at: 4517 5969 6055 6067 6413 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 641 435 385 367 346 274 259 167 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2345 2679 3679 2345 Size: 334 1000 5084 Fragments arranged by size: 5084 1000 334 BtgI C'CryG_G Cuts at: 513 1065 2004 2649 513 Size: 552 939 645 4282 Fragments arranged by size: 4282 939 645 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2737 487 Size: 683 56 1511 4168 Fragments arranged by size: 4168 1511 683 56 BtsI GCAGTG_nn' Cuts at: 2493 2589 3934 4728 4748 2493 Size: 96 1345 794 20 4163 Fragments arranged by size: 4163 1345 794 96 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6383 DraI TTT'AAA Cuts at: 3722 3722 Size: 6418 DraIII CAC_nnn'GTG Cuts at: 2833 2833 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5425 809 Size: 507 4109 1802 Fragments arranged by size: 4109 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2120 2646 3167 Size: 54 1425 50 275 18 290 526 521 Cuts at: 3167 3865 4754 6383 8 Size: 698 889 1629 43 Fragments arranged by size: 1629 1425 889 698 526 521 290 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2107 2955 4348 1353 Size: 705 49 848 1393 3423 Fragments arranged by size: 3423 1393 848 705 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5005 5379 5525 387 Size: 1064 138 3416 374 146 1280 Fragments arranged by size: 3416 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2138 2138 Size: 6418 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2951 3542 3645 4481 5078 5865 Size: 393 603 716 591 103 836 597 787 Cuts at: 5865 6133 1239 Size: 268 1524 Fragments arranged by size: 1524 836 787 716 603 597 591 393 268 103 EcoO109I rG'GnC_Cy Cuts at: 2530 3223 3305 3447 3585 2530 Size: 693 82 142 138 5363 Fragments arranged by size: 5363 693 142 138 82 EcoRI G'AATT_C Cuts at: 2023 3727 4105 2023 Size: 1704 378 4336 Fragments arranged by size: 4336 1704 378 EcoRV GAT'ATC Cuts at: 3115 3115 Size: 6418 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 5565 1678 Size: 3887 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 2708 2740 3072 Size: 32 135 32 950 32 154 32 332 Cuts at: 3072 3104 5059 5091 5133 5165 1373 Size: 32 1955 32 42 32 2626 Fragments arranged by size: 2626 1955 950 332 154 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3202 3762 4008 669 Size: 988 281 1264 560 246 3079 Fragments arranged by size: 3079 1264 988 560 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6418 HpaI GTT'AAC Cuts at: 4008 4008 Size: 6418 KpnI G_GTAC'C Cuts at: 3743 3743 Size: 6418 MfeI C'AATT_G Cuts at: 2028 2378 4017 2028 Size: 350 1639 4429 Fragments arranged by size: 4429 1639 350 MluI A'CGCG_T Cuts at: 2165 6294 2165 Size: 4129 2289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5532 5716 1305 Size: 4227 184 2007 Fragments arranged by size: 4227 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2122 10 Size: 54 1425 343 290 4306 Fragments arranged by size: 4306 1425 343 290 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 4376 4735 4894 518 Size: 648 1320 1890 359 159 2042 Fragments arranged by size: 2042 1890 1320 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3521 3834 4511 5659 5904 1606 Size: 1915 313 677 1148 245 2120 Fragments arranged by size: 2120 1915 1148 677 313 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4927 Fragments arranged by size: 4927 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 2299 2387 3776 5321 1132 Size: 97 1070 88 1389 1545 2229 Fragments arranged by size: 2229 1545 1389 1070 97 88 PasI CC'CwG_GG Cuts at: 1348 3589 3700 1348 Size: 2241 111 4066 Fragments arranged by size: 4066 2241 111 PciI A'CATG_T Cuts at: 5317 5317 Size: 6418 PflMI CCAn_nnn'nTGG Cuts at: 1108 2830 3176 1108 Size: 1722 346 4350 Fragments arranged by size: 4350 1722 346 PfoI T'CCnGG_A Cuts at: 2408 3558 2408 Size: 1150 5268 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PmlI CAC'GTG Cuts at: 2048 2048 Size: 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4487 4519 6000 6032 4487 Size: 32 1481 32 4873 Fragments arranged by size: 4873 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2530 3223 2530 Size: 693 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6418 PsiI TTA'TAA Cuts at: 2144 3988 2144 Size: 1844 4574 PspOMI G'GGCC_C Cuts at: 3305 3446 3305 Size: 141 6277 PstI C_TGCA'G Cuts at: 830 2584 2988 3770 830 Size: 1754 404 782 3478 Fragments arranged by size: 3478 1754 782 404 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 PvuII CAG'CTG Cuts at: 3521 3521 Size: 6418 SacI G_AGCT'C Cuts at: 2140 2140 Size: 6418 SalI G'TCGA_C Cuts at: 1936 3760 1936 Size: 1824 4594 SapI GCTCTTCn'nnn_ Cuts at: 2955 2955 Size: 6418 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 4666 6378 4666 Size: 1712 4706 SfcI C'TryA_G Cuts at: 826 1045 2580 2951 2984 3766 4901 5582 5773 Size: 219 1535 371 33 782 1135 681 191 Cuts at: 5773 826 Size: 1471 Fragments arranged by size: 1535 1471 1135 782 681 371 219 191 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 3360 1700 Size: 1660 4758 SmaI CCC'GGG Cuts at: 1872 3745 1872 Size: 1873 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 3470 3525 4522 5423 5685 Size: 197 926 318 1209 55 997 901 262 Cuts at: 5685 820 Size: 1553 Fragments arranged by size: 1553 1209 997 926 901 318 262 197 55 SnaBI TAC'GTA Cuts at: 493 4209 493 Size: 3716 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 2387 3776 1132 Size: 1255 1389 3774 Fragments arranged by size: 3774 1389 1255 SspI AAT'ATT Cuts at: 5 52 4342 5 Size: 47 4290 2081 Fragments arranged by size: 4290 2081 47 StuI AGG'CCT Cuts at: 1517 3690 1517 Size: 2173 4245 StyI C'CwwG_G Cuts at: 513 1065 2004 2450 3857 513 Size: 552 939 446 1407 3074 Fragments arranged by size: 3074 1407 939 552 446 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2345 4445 4462 4615 4800 5245 325 Size: 1255 765 2100 17 153 185 445 1498 Fragments arranged by size: 2100 1498 1255 765 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2308 3474 4664 Size: 274 80 33 51 1443 331 1166 1190 Cuts at: 4664 6376 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1190 1166 331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6378 6410 6378 Size: 32 6386 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 3754 3754 Size: 6418 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3004 3566 3004 Size: 562 5856 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6418 XmaI C'CCGG_G Cuts at: 1870 3743 1870 Size: 1873 4545 XmnI GAAnn'nnTTC Cuts at: 4547 4547 Size: 6418 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI