Restriction map for
pFN21AE0977
(Linear) (Six-base) MAPSORT of: KIEE0977.seq Check: 6716 from: 1 to: 1713
KIEE0977.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 1713
Size: 230 1483
AflII C'TTAA_G
Cuts at: 0 256 1713
Size: 256 1457
AflIII A'CryG_T
Cuts at: 0 42 160 1713
Size: 42 118 1553
Fragments arranged by size:
1553 118 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1702 1713
Size: 1702 11
ApaI G_GGCC'C
Cuts at: 0 1304 1445 1713
Size: 1304 141 268
Fragments arranged by size:
1304 268 141
ApoI r'AATT_y
Cuts at: 0 18 792 932 1713
Size: 18 774 140 781
Fragments arranged by size:
781 774 140 18
BamHI G'GATC_C
Cuts at: 0 882 1713
Size: 882 831
BanI G'GyrC_C
Cuts at: 0 669 1713
Size: 669 1044
BanII G_rGCy'C
Cuts at: 0 135 695 1304 1337 1445 1713
Size: 135 560 609 33 108 268
Fragments arranged by size:
609 560 268 135 108 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 925 1713
Size: 925 788
BbvCI CC'TCA_GC
Cuts at: 0 490 1713
Size: 490 1223
BglI GCCn_nnn'nGGC
Cuts at: 0 1531 1713
Size: 1531 182
Bme1580I G_kGCm'C
Cuts at: 0 1304 1445 1713
Size: 1304 141 268
Fragments arranged by size:
1304 268 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1537 1640 1713
Size: 946 591 103 73
Fragments arranged by size:
946 591 103 73
Bpu10I CC'TnA_GC
Cuts at: 0 490 1713
Size: 490 1223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1450 1505 1713
Size: 1450 55 208
Fragments arranged by size:
1450 208 55
BsaAI yAC'GTr
Cuts at: 0 43 1132 1713
Size: 43 1089 581
Fragments arranged by size:
1089 581 43
BsaWI w'CCGG_w
Cuts at: 0 768 1713
Size: 768 945
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 95 1633 1713
Size: 95 1538 80
Fragments arranged by size:
1538 95 80
BseYI C'CCAG_C
Cuts at: 0 1255 1713
Size: 1255 458
BsiHKAI G_wGCw'C
Cuts at: 0 135 1212 1713
Size: 135 1077 501
Fragments arranged by size:
1077 501 135
Bsp1286I G_dGCh'C
Cuts at: 0 135 695 1212 1304 1337 1445 1713
Size: 135 560 517 92 33 108 268
Fragments arranged by size:
560 517 268 135 108 92 33
BspEI T'CCGG_A
Cuts at: 0 768 1713
Size: 768 945
BspHI T'CATG_A
Cuts at: 0 311 1713
Size: 311 1402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 443 1259 1713
Size: 443 816 454
Fragments arranged by size:
816 454 443
BsrFI r'CCGG_y
Cuts at: 0 1160 1355 1713
Size: 1160 195 358
Fragments arranged by size:
1160 358 195
BssSI C'ACGA_G
Cuts at: 0 1166 1713
Size: 1166 547
BstYI r'GATC_y
Cuts at: 0 608 882 1049 1484 1713
Size: 608 274 167 435 229
Fragments arranged by size:
608 435 274 229 167
Bsu36I CC'TnA_GG
Cuts at: 0 340 674 1674 1713
Size: 340 334 1000 39
Fragments arranged by size:
1000 340 334 39
BtgI C'CryG_G
Cuts at: 0 644 1713
Size: 644 1069
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 732 1713
Size: 732 981
BtsI GCAGTG_nn'
Cuts at: 0 488 584 1713
Size: 488 96 1129
Fragments arranged by size:
1129 488 96
DraIII CAC_nnn'GTG
Cuts at: 0 828 1713
Size: 828 885
EaeI y'GGCC_r
Cuts at: 0 115 641 1162 1713
Size: 115 526 521 551
Fragments arranged by size:
551 526 521 115
EarI CTCTTCn'nnn_
Cuts at: 0 53 102 950 1713
Size: 53 49 848 763
Fragments arranged by size:
848 763 53 49
EcoICRI GAG'CTC
Cuts at: 0 133 1713
Size: 133 1580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 946 1537 1640 1713
Size: 230 716 591 103 73
Fragments arranged by size:
716 591 230 103 73
EcoO109I rG'GnC_Cy
Cuts at: 0 525 1218 1300 1442 1580 1713
Size: 525 693 82 142 138 133
Fragments arranged by size:
693 525 142 138 133 82
EcoRI G'AATT_C
Cuts at: 0 18 1713
Size: 18 1695
EcoRV GAT'ATC
Cuts at: 0 1110 1713
Size: 1110 603
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 703 735 1067 1099 1713
Size: 517 32 154 32 332 32 614
Fragments arranged by size:
614 517 332 154 32 32 32
HincII GTy'rAC
Cuts at: 0 1197 1713
Size: 1197 516
MfeI C'AATT_G
Cuts at: 0 23 373 1713
Size: 23 350 1340
Fragments arranged by size:
1340 350 23
MluI A'CGCG_T
Cuts at: 0 160 1713
Size: 160 1553
MscI TGG'CCA
Cuts at: 0 117 1713
Size: 117 1596
MslI CAynn'nnrTG
Cuts at: 0 481 1713
Size: 481 1232
MspA1I CmG'CkG
Cuts at: 0 1516 1713
Size: 1516 197
NspI r_CATG'y
Cuts at: 0 294 382 1713
Size: 294 88 1331
Fragments arranged by size:
1331 294 88
PasI CC'CwG_GG
Cuts at: 0 1584 1695 1713
Size: 1584 111 18
Fragments arranged by size:
1584 111 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 825 1171 1713
Size: 825 346 542
Fragments arranged by size:
825 542 346
PfoI T'CCnGG_A
Cuts at: 0 403 1553 1713
Size: 403 1150 160
Fragments arranged by size:
1150 403 160
PmlI CAC'GTG
Cuts at: 0 43 1713
Size: 43 1670
PpuMI rG'GwC_Cy
Cuts at: 0 525 1218 1713
Size: 525 693 495
Fragments arranged by size:
693 525 495
PsiI TTA'TAA
Cuts at: 0 139 1713
Size: 139 1574
PspOMI G'GGCC_C
Cuts at: 0 1300 1441 1713
Size: 1300 141 272
Fragments arranged by size:
1300 272 141
PstI C_TGCA'G
Cuts at: 0 579 983 1713
Size: 579 404 730
Fragments arranged by size:
730 579 404
PvuII CAG'CTG
Cuts at: 0 1516 1713
Size: 1516 197
SacI G_AGCT'C
Cuts at: 0 135 1713
Size: 135 1578
SapI GCTCTTCn'nnn_
Cuts at: 0 950 1713
Size: 950 763
SfcI C'TryA_G
Cuts at: 0 575 946 979 1713
Size: 575 371 33 734
Fragments arranged by size:
734 575 371 33
SgrAI Cr'CCGG_yG
Cuts at: 0 1355 1713
Size: 1355 358
SmlI C'TyrA_G
Cuts at: 0 256 1465 1520 1713
Size: 256 1209 55 193
Fragments arranged by size:
1209 256 193 55
SphI G_CATG'C
Cuts at: 0 382 1713
Size: 382 1331
StuI AGG'CCT
Cuts at: 0 1685 1713
Size: 1685 28
StyI C'CwwG_G
Cuts at: 0 445 1713
Size: 445 1268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 340 1713
Size: 340 1373
TatI w'GTAC_w
Cuts at: 0 303 1469 1713
Size: 303 1166 244
Fragments arranged by size:
1166 303 244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 999 1561 1713
Size: 999 562 152
Fragments arranged by size:
999 562 152
Enzymes that do cut:
AcuI AflII AflIII AlfI ApaI ApoI BamHI BanI
BanII BbsI BbvCI BglI Bme1580I BpmI Bpu10I BpuEI
BsaAI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrFI BssSI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HincII MfeI MluI MscI MslI MspA1I NspI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI
PvuII SacI SapI SfcI SgrAI SmlI SphI StuI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI
CspCI DraI DrdI EagI EciI EcoNI FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SnaBI SpeI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI