Restriction map for
pFN21AE0963
(Circular) (Six-base) MAPSORT of: pFN21AE0963.seq Check: 6923 from: 1 to: 6172
pFN21AE0963 6172 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 1937
Size: 1578 4594
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2238 2325 2678 3315 3455 4235 5619 5887
Size: 606 87 353 637 140 780 1384 268
Cuts at: 5887 1632
Size: 1917
Fragments arranged by size:
1917 1384 780 637 606 353 268 140
87
AflII C'TTAA_G
Cuts at: 820 1017 2597 3209 820
Size: 197 1580 612 3783
Fragments arranged by size:
3783 1580 612 197
AflIII A'CryG_T
Cuts at: 1203 2368 5071 6048 1203
Size: 1165 2703 977 1327
Fragments arranged by size:
2703 1327 1165 977
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 2560 3424 4901 2560
Size: 864 1477 3831
Fragments arranged by size:
3831 1477 864
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2682 2714 2998 3030 2682
Size: 32 284 32 5824
Fragments arranged by size:
5824 284 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3915 5487 3915
Size: 1572 4600
ApaLI G'TGCA_C
Cuts at: 4229 5385 4229
Size: 1156 5016
ApoI r'AATT_y
Cuts at: 1493 2140 2520 2851 2938 3481 3707 3859 5968
Size: 647 380 331 87 543 226 152 2109
Cuts at: 5968 1493
Size: 1697
Fragments arranged by size:
2109 1697 647 543 380 331 226 152
87
AseI AT'TA_AT
Cuts at: 160 1032 2931 4726 160
Size: 872 1899 1795 1606
Fragments arranged by size:
1899 1795 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2763 2804 3497 1870
Size: 73 820 41 693 4545
Fragments arranged by size:
4545 820 693 73 41
BamHI G'GATC_C
Cuts at: 3349 3502 3869 3349
Size: 153 367 5652
Fragments arranged by size:
5652 367 153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3493 4948 618
Size: 325 217 627 1706 1455 1842
Fragments arranged by size:
1842 1706 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2059 3491 1391
Size: 654 14 1432 4072
Fragments arranged by size:
4072 1432 654 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 2447 928
Size: 971 414 134 4653
Fragments arranged by size:
4653 971 414 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 1903
Size: 34 2426 34 3678
Fragments arranged by size:
3678 2426 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BfrBI ATG'CAT
Cuts at: 3086 3086
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4783 136
Size: 107 122 71 631 605 3111 1525
Fragments arranged by size:
3111 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 3589 3589
Size: 6172
Bme1580I G_kGCm'C
Cuts at: 1790 4233 5389 1790
Size: 2443 1156 2573
Fragments arranged by size:
2573 2443 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3430 3903 4861
Size: 416 53 83 399 24 2013 473 958
Cuts at: 4861 442
Size: 1753
Fragments arranged by size:
2013 1753 958 473 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2499 3085 4832 1239
Size: 1260 586 1747 2579
Fragments arranged by size:
2579 1747 1260 586
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3383 4297 5162 5460 3383
Size: 914 865 298 4095
Fragments arranged by size:
4095 914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3176 4835 882
Size: 617 1677 1659 2219
Fragments arranged by size:
2219 1677 1659 617
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 6166 6166
Size: 6172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3979 4361 4996 275
Size: 53 83 186 936 2446 382 635 1451
Fragments arranged by size:
2446 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4605 5003 5277 5424 5835 4605
Size: 398 274 147 411 4942
Fragments arranged by size:
4942 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5947
Fragments arranged by size:
5947 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3395 3413 1186
Size: 2209 18 3945
Fragments arranged by size:
3945 2209 18
BseYI C'CCAG_C
Cuts at: 5375 5871 5375
Size: 496 5676
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5599
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3622 4383 5411 1540
Size: 275 186 1621 761 1028 2301
Fragments arranged by size:
2301 1621 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2059 4233 4318 5389 2059
Size: 2174 85 1071 2842
Fragments arranged by size:
2842 2174 1071 85
BsmI GAATG_Cn'
Cuts at: 3220 3683 3776 3220
Size: 463 93 5616
Fragments arranged by size:
5616 463 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2679 2679
Size: 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2059 3491 4233 4318 5389 1391
Size: 399 255 14 1432 742 85 1071 2174
Fragments arranged by size:
2174 1432 1071 742 399 255 85 14
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 4061 5791 4061
Size: 1730 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 3527 844
Size: 1697 986 3489
Fragments arranged by size:
3489 1697 986
BsrBI CCG'CTC
Cuts at: 1394 1823 4059 1394
Size: 429 2236 3507
Fragments arranged by size:
3507 2236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4816 5003 1700
Size: 110 3006 187 2869
Fragments arranged by size:
3006 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6172
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6172
BstBI TT'CG_AA
Cuts at: 2855 4976 2855
Size: 2121 4051
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 2523 2470
Size: 53 6119
BstYI r'GATC_y
Cuts at: 1972 2260 2444 3349 3502 3869 4254 4271 5723
Size: 288 184 905 153 367 385 17 1452
Cuts at: 5723 5809 5821 6167 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 905 385 367 346 288 184
153 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4681
Fragments arranged by size:
4681 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2720 487
Size: 683 56 1494 3939
Fragments arranged by size:
3939 1494 683 56
BtsI GCAGTG_nn'
Cuts at: 2666 3176 3688 4482 4502 2666
Size: 510 512 794 20 4336
Fragments arranged by size:
4336 794 512 510 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 2719 3476 2719
Size: 757 5415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2018 5179 809
Size: 507 702 3161 1802
Fragments arranged by size:
3161 1802 702 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3619 4508 6137
Size: 54 1425 50 275 18 1789 889 1629
Cuts at: 6137 8
Size: 43
Fragments arranged by size:
1789 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3619 1537
Size: 275 1807 4090
Fragments arranged by size:
4090 1807 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2956 4102 1353
Size: 1603 1146 3423
Fragments arranged by size:
3423 1603 1146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2057 2057
Size: 6172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2238 2325 2499 2678 3085 3315 3455
Size: 393 606 87 174 179 407 230 140
Cuts at: 3455 4235 4832 5619 5887 1239
Size: 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 606 597 407 393 268
230 179 174 140 87
EcoO109I rG'GnC_Cy
Cuts at: 3284 3284
Size: 6172
EcoRI G'AATT_C
Cuts at: 2140 3481 3859 2140
Size: 1341 378 4453
Fragments arranged by size:
4453 1341 378
EcoRV GAT'ATC
Cuts at: 2386 2386
Size: 6172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 6172
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 5319 1678
Size: 3641 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2429 2461 2546
Size: 32 135 32 803 32 22 32 85
Cuts at: 2546 2578 3410 3442 4813 4845 4887 4919 1373
Size: 32 832 32 1371 32 42 32 2626
Fragments arranged by size:
2626 1371 832 803 135 85 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3516 3762 669
Size: 988 281 1578 246 3079
Fragments arranged by size:
3079 1578 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2603 5286 5470 1305
Size: 1298 2683 184 2007
Fragments arranged by size:
2683 2007 1298 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4350
Fragments arranged by size:
4350 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4130 4489 4648 518
Size: 648 2964 359 159 2042
Fragments arranged by size:
2964 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3588 4265 5413 5658 1606
Size: 1982 677 1148 245 2120
Fragments arranged by size:
2120 1982 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4681
Fragments arranged by size:
4681 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NsiI A_TGCA'T
Cuts at: 3088 3088
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 3530 5075 1132
Size: 97 2301 1545 2229
Fragments arranged by size:
2301 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6172
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6172
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 2541 3742 2541
Size: 1201 4971
PstI C_TGCA'G
Cuts at: 830 3524 830
Size: 2694 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
SacI G_AGCT'C
Cuts at: 2059 2059
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SexAI A'CCwGG_T
Cuts at: 3259 3259
Size: 6172
SfcI C'TryA_G
Cuts at: 826 1045 3520 4655 5336 5527 826
Size: 219 2475 1135 681 191 1471
Fragments arranged by size:
2475 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 3499 1872
Size: 1627 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 2763 3209 3362 4276 5177
Size: 197 926 654 166 446 153 914 901
Cuts at: 5177 5439 820
Size: 262 1553
Fragments arranged by size:
1553 926 914 901 654 446 262 197
166 153
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SspI AAT'ATT
Cuts at: 5 52 2188 4096 5
Size: 47 2136 1908 2081
Fragments arranged by size:
2136 2081 1908 47
StuI AGG'CCT
Cuts at: 1517 3459 1517
Size: 1942 4230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2341 2565 3611 513
Size: 552 939 337 224 1046 3074
Fragments arranged by size:
3074 1046 939 552 337 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3412 4199 4216 4369 4554 4999 325
Size: 1255 1832 787 17 153 185 445 1498
Fragments arranged by size:
1832 1498 1255 787 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3144 4418 6130
Size: 274 80 33 51 1443 1167 1274 1712
Cuts at: 6130 96
Size: 138
Fragments arranged by size:
1712 1443 1274 1167 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2339 2339
Size: 6172
XhoI C'TCGA_G
Cuts at: 1943 2763 1943
Size: 820 5352
XmaI C'CCGG_G
Cuts at: 1870 3497 1870
Size: 1627 4545
XmnI GAAnn'nnTTC
Cuts at: 2402 4301 2402
Size: 1899 4273
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI