(Circular) (Six-base) MAPSORT of: pFN21AE0960.seq Check: 8980 from: 1 to: 6202 pFN21AE0960 6202 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2982 3545 1937 Size: 1045 563 4594 Fragments arranged by size: 4594 1045 563 Acc65I G'GTAC_C Cuts at: 2022 3523 2022 Size: 1501 4701 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 3410 4265 5649 5917 1632 Size: 968 810 855 1384 268 1917 Fragments arranged by size: 1917 1384 968 855 810 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 2718 5101 6078 1203 Size: 1515 2383 977 1327 Fragments arranged by size: 2383 1515 1327 977 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 4931 4931 Size: 6202 AleI CACnn'nnGTG Cuts at: 1166 2969 1166 Size: 1803 4399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2987 3332 1184 Size: 1803 345 4054 Fragments arranged by size: 4054 1803 345 AlwNI CAG_nnn'CTG Cuts at: 3945 5517 3945 Size: 1572 4630 ApaLI G'TGCA_C Cuts at: 4259 5415 4259 Size: 1156 5046 ApoI r'AATT_y Cuts at: 1493 2902 3511 3737 3889 5998 1493 Size: 1409 609 226 152 2109 1697 Fragments arranged by size: 2109 1697 1409 609 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2568 3527 1870 Size: 73 625 959 4545 Fragments arranged by size: 4545 959 625 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2707 2740 2707 Size: 33 6169 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 3523 4978 618 Size: 325 217 627 235 1501 1455 1842 Fragments arranged by size: 1842 1501 1455 627 325 235 217 BanII G_rGCy'C Cuts at: 1391 2569 2919 3521 1391 Size: 1178 350 602 4072 Fragments arranged by size: 4072 1178 602 350 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5231 BbvCI CC'TCA_GC Cuts at: 2445 2445 Size: 6202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4393 4427 1903 Size: 34 50 34 2372 34 3678 Fragments arranged by size: 3678 2372 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4094 5304 4094 Size: 1210 4992 BclI T'GATC_A Cuts at: 1548 1548 Size: 6202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4813 136 Size: 107 122 71 631 605 3141 1525 Fragments arranged by size: 3141 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6197 6197 Size: 6202 BlpI GC'TnA_GC Cuts at: 3399 3456 3619 3399 Size: 57 163 5982 Fragments arranged by size: 5982 163 57 Bme1580I G_kGCm'C Cuts at: 1790 4263 5419 1790 Size: 2473 1156 2573 Fragments arranged by size: 2573 2473 1156 BmgBI CAC'GTC Cuts at: 1318 2467 1318 Size: 1149 5053 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3933 4891 442 Size: 416 53 83 399 24 2516 958 1753 Fragments arranged by size: 2516 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3466 4862 1239 Size: 2227 1396 2579 Fragments arranged by size: 2579 2227 1396 Bpu10I CC'TnA_GC Cuts at: 2445 2747 2445 Size: 302 5900 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 2861 3086 4327 5192 5490 2645 Size: 216 225 1241 865 298 3357 Fragments arranged by size: 3357 1241 865 298 225 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4865 882 Size: 617 3366 2219 Fragments arranged by size: 3366 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3993 493 Size: 711 2789 2702 Fragments arranged by size: 2789 2702 711 BsaBI GATnn'nnATC Cuts at: 6196 6196 Size: 6202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2137 4009 4391 5026 Size: 53 83 186 936 604 1872 382 635 Cuts at: 5026 275 Size: 1451 Fragments arranged by size: 1872 1451 936 635 604 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 2199 4635 5033 5307 5454 5865 2124 Size: 75 2436 398 274 147 411 2461 Fragments arranged by size: 2461 2436 411 398 274 147 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2044 2074 1178 Size: 30 165 30 641 30 5306 Fragments arranged by size: 5306 641 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2020 2383 1186 Size: 834 363 5005 Fragments arranged by size: 5005 834 363 BseYI C'CCAG_C Cuts at: 3284 5405 5901 3284 Size: 2121 496 3585 Fragments arranged by size: 3585 2121 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5629 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2200 2530 3652 4413 5441 1540 Size: 275 186 199 330 1122 761 1028 2301 Fragments arranged by size: 2301 1122 1028 761 330 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2919 2966 3015 3052 4263 4348 5419 2919 Size: 47 49 37 1211 85 1071 3702 Fragments arranged by size: 3702 1211 1071 85 49 47 37 BsmI GAATG_Cn' Cuts at: 2293 3713 3806 2293 Size: 1420 93 4689 Fragments arranged by size: 4689 1420 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2569 2919 2966 3015 3052 3521 4263 Size: 399 779 350 47 49 37 469 742 Cuts at: 4263 4348 5419 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 779 742 469 399 350 85 49 47 37 BspEI T'CCGG_A Cuts at: 5865 5865 Size: 6202 BspHI T'CATG_A Cuts at: 4091 5821 4091 Size: 1730 4472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2790 3557 844 Size: 1946 767 3489 Fragments arranged by size: 3489 1946 767 BsrBI CCG'CTC Cuts at: 1394 1823 2394 4089 1394 Size: 429 571 1695 3507 Fragments arranged by size: 3507 1695 571 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4697 4871 65 Size: 1183 3449 174 1396 Fragments arranged by size: 3449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2214 4846 5033 1700 Size: 110 404 2632 187 2869 Fragments arranged by size: 2869 2632 404 187 110 BsrGI T'GTAC_A Cuts at: 96 2536 96 Size: 2440 3762 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6202 BssSI C'ACGA_G Cuts at: 4262 5274 4262 Size: 1012 5190 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6202 BstBI TT'CG_AA Cuts at: 5006 5006 Size: 6202 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6202 BstYI r'GATC_y Cuts at: 1972 3189 3532 3899 4284 4301 5753 5839 5851 Size: 1217 343 367 385 17 1452 86 12 Cuts at: 5851 6197 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1217 385 367 346 343 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2509 2509 Size: 6202 BtgI C'CryG_G Cuts at: 513 1065 2710 513 Size: 552 1645 4005 Fragments arranged by size: 4005 1645 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2537 487 Size: 683 56 1311 4152 Fragments arranged by size: 4152 1311 683 56 BtsI GCAGTG_nn' Cuts at: 2959 2998 3718 4512 4532 2959 Size: 39 720 794 20 4629 Fragments arranged by size: 4629 794 720 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DraIII CAC_nnn'GTG Cuts at: 2803 2803 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2097 5209 809 Size: 507 781 3112 1802 Fragments arranged by size: 3112 1802 781 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2527 2713 3649 Size: 54 1425 50 275 18 697 186 936 Cuts at: 3649 4538 6167 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 936 889 697 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2527 3649 1537 Size: 275 715 1122 4090 Fragments arranged by size: 4090 1122 715 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2954 3149 3365 4132 1353 Size: 1601 195 216 767 3423 Fragments arranged by size: 3423 1601 767 216 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2380 4789 5163 5309 387 Size: 1064 138 791 2409 374 146 1280 Fragments arranged by size: 2409 1280 1064 791 374 146 138 EcoICRI GAG'CTC Cuts at: 2917 2917 Size: 6202 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2600 3410 3466 4265 4862 5649 5917 Size: 393 968 810 56 799 597 787 268 Cuts at: 5917 1239 Size: 1524 Fragments arranged by size: 1524 968 810 799 787 597 393 268 56 EcoO109I rG'GnC_Cy Cuts at: 2520 3197 2520 Size: 677 5525 EcoRI G'AATT_C Cuts at: 2902 3511 3889 2902 Size: 609 378 5215 Fragments arranged by size: 5215 609 378 EcoRV GAT'ATC Cuts at: 3493 3493 Size: 6202 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3080 3080 Size: 6202 FspI TGC'GCA Cuts at: 4708 4708 Size: 6202 HaeII r_GCGC'y Cuts at: 1678 3292 5349 1678 Size: 1614 2057 2531 Fragments arranged by size: 2531 2057 1614 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2481 2513 2613 Size: 32 135 32 605 32 272 32 100 Cuts at: 2613 2645 3126 3158 3181 3213 3263 3295 4843 Size: 32 481 32 23 32 50 32 1548 Cuts at: 4843 4875 4917 4949 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1548 605 481 272 135 100 50 42 32 32 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2548 3546 3792 669 Size: 988 281 610 998 246 3079 Fragments arranged by size: 3079 998 988 610 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6202 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KpnI G_GTAC'C Cuts at: 2026 3527 2026 Size: 1501 4701 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2220 5316 5500 1305 Size: 915 3096 184 2007 Fragments arranged by size: 3096 2007 915 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2715 10 Size: 54 1425 343 883 3497 Fragments arranged by size: 3497 1425 883 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 2969 4160 4519 4678 518 Size: 648 1132 671 1191 359 159 2042 Fragments arranged by size: 2042 1191 1132 671 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 2824 2969 3284 3316 3618 4295 5443 Size: 705 513 145 315 32 302 677 1148 Cuts at: 5443 5688 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 705 677 513 315 302 245 145 32 NcoI C'CATG_G Cuts at: 513 1065 2710 513 Size: 552 1645 4005 Fragments arranged by size: 4005 1645 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 2722 3382 3560 5105 1132 Size: 97 1493 660 178 1545 2229 Fragments arranged by size: 2229 1545 1493 660 178 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6202 PciI A'CATG_T Cuts at: 2718 5101 2718 Size: 2383 3819 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6202 PfoI T'CCnGG_A Cuts at: 3209 3209 Size: 6202 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 5784 5816 4271 Size: 32 1481 32 4657 Fragments arranged by size: 4657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2520 3197 2520 Size: 677 5525 PshAI GACnn'nnGTC Cuts at: 1613 3483 1613 Size: 1870 4332 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PspXI vC'TCGA_Gb Cuts at: 2568 2568 Size: 6202 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2309 2341 2309 Size: 32 6170 PstI C_TGCA'G Cuts at: 830 3554 830 Size: 2724 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6202 PvuII CAG'CTG Cuts at: 2824 2969 3284 2824 Size: 145 315 5742 Fragments arranged by size: 5742 315 145 SacI G_AGCT'C Cuts at: 2919 2919 Size: 6202 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SapI GCTCTTCn'nnn_ Cuts at: 2954 3365 2954 Size: 411 5791 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 4450 6162 4450 Size: 1712 4490 SfcI C'TryA_G Cuts at: 826 1045 2233 3550 4685 5366 5557 826 Size: 219 1188 1317 1135 681 191 1471 Fragments arranged by size: 1471 1317 1188 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 3529 1872 Size: 1657 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2568 2624 2876 3065 4306 5207 Size: 197 926 625 56 252 189 1241 901 Cuts at: 5207 5469 820 Size: 262 1553 Fragments arranged by size: 1553 1241 926 901 625 262 252 197 189 56 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3382 3560 1132 Size: 2250 178 3774 Fragments arranged by size: 3774 2250 178 SspI AAT'ATT Cuts at: 5 52 2890 3242 4126 5 Size: 47 2838 352 884 2081 Fragments arranged by size: 2838 2081 884 352 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6202 StyI C'CwwG_G Cuts at: 513 1065 2710 3411 3641 513 Size: 552 1645 701 230 3074 Fragments arranged by size: 3074 1645 701 552 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 4229 4246 4399 4584 5029 325 Size: 1255 1824 825 17 153 185 445 1498 Fragments arranged by size: 1824 1498 1255 825 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2536 2604 4448 Size: 274 80 33 51 1443 559 68 1844 Cuts at: 4448 6160 96 Size: 1712 138 Fragments arranged by size: 1844 1712 1443 559 274 138 80 68 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2044 2076 6162 6194 2044 Size: 32 4086 32 2052 Fragments arranged by size: 4086 2052 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2136 1316 Size: 820 5382 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2558 2558 Size: 6202 XhoI C'TCGA_G Cuts at: 1943 2568 1943 Size: 625 5577 XmaI C'CCGG_G Cuts at: 1870 3527 1870 Size: 1657 4545 XmnI GAAnn'nnTTC Cuts at: 2889 3423 4331 2889 Size: 534 908 4760 Fragments arranged by size: 4760 908 534 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI