(Circular) (Six-base) MAPSORT of: pFN21AE0959.seq Check: 3101 from: 1 to: 6202 pFN21AE0959 6202 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3369 3369 Size: 6202 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3545 1937 Size: 1608 4594 Acc65I G'GTAC_C Cuts at: 3523 3523 Size: 6202 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4265 5649 5917 1632 Size: 2633 1384 268 1917 Fragments arranged by size: 2633 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 5101 6078 1203 Size: 3898 977 1327 Fragments arranged by size: 3898 1327 977 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 4931 4931 Size: 6202 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6202 AlwNI CAG_nnn'CTG Cuts at: 2114 2689 2792 2947 3160 3223 3294 3945 5517 Size: 575 103 155 213 63 71 651 1572 Cuts at: 5517 2114 Size: 2799 Fragments arranged by size: 2799 1572 651 575 213 155 103 71 63 ApaI G_GGCC'C Cuts at: 2447 2461 2580 2733 3148 3230 2447 Size: 14 119 153 415 82 5419 Fragments arranged by size: 5419 415 153 119 82 14 ApaLI G'TGCA_C Cuts at: 2693 3382 4259 5415 2693 Size: 689 877 1156 3480 Fragments arranged by size: 3480 1156 877 689 ApoI r'AATT_y Cuts at: 1493 3511 3737 3889 5998 1493 Size: 2018 226 152 2109 1697 Fragments arranged by size: 2109 2018 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2733 2831 3432 3527 1870 Size: 73 790 98 601 95 4545 Fragments arranged by size: 4545 790 601 98 95 73 AvrII C'CTAG_G Cuts at: 3310 3310 Size: 6202 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2072 2226 2609 3523 Size: 325 217 627 264 21 154 383 914 Cuts at: 3523 4978 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 914 627 383 325 264 217 154 21 BanII G_rGCy'C Cuts at: 1391 2145 2288 2447 2461 2580 2733 2849 3148 Size: 754 143 159 14 119 153 116 299 Cuts at: 3148 3230 3440 3521 1391 Size: 82 210 81 4072 Fragments arranged by size: 4072 754 299 210 159 153 143 119 116 82 81 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2368 2982 928 Size: 971 469 614 4148 Fragments arranged by size: 4148 971 614 469 BbvCI CC'TCA_GC Cuts at: 3470 3470 Size: 6202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4393 4427 1903 Size: 34 50 34 2372 34 3678 Fragments arranged by size: 3678 2372 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4094 5304 4094 Size: 1210 4992 BclI T'GATC_A Cuts at: 1548 1548 Size: 6202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2140 2352 3065 Size: 107 122 71 631 605 468 212 713 Cuts at: 3065 4813 136 Size: 1748 1525 Fragments arranged by size: 1748 1525 713 631 605 468 212 122 107 71 BglII A'GATC_T Cuts at: 6197 6197 Size: 6202 BlpI GC'TnA_GC Cuts at: 2144 2342 3619 2144 Size: 198 1277 4727 Fragments arranged by size: 4727 1277 198 Bme1580I G_kGCm'C Cuts at: 1790 2054 2447 2461 2580 2697 2733 3148 3230 Size: 264 393 14 119 117 36 415 82 Cuts at: 3230 3386 4263 5419 1790 Size: 156 877 1156 2573 Fragments arranged by size: 2573 1156 877 415 393 264 156 119 117 82 36 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 3304 3933 Size: 416 53 83 399 24 661 1226 629 Cuts at: 3933 4891 442 Size: 958 1753 Fragments arranged by size: 1753 1226 958 661 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2543 3044 3271 4862 1239 Size: 1304 501 227 1591 2579 Fragments arranged by size: 2579 1591 1304 501 227 Bpu10I CC'TnA_GC Cuts at: 3131 3470 3131 Size: 339 5863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2111 4327 5192 5490 2111 Size: 2216 865 298 2823 Fragments arranged by size: 2823 2216 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2805 4865 882 Size: 617 1306 2060 2219 Fragments arranged by size: 2219 2060 1306 617 BsaAI yAC'GTr Cuts at: 493 1204 3993 493 Size: 711 2789 2702 Fragments arranged by size: 2789 2702 711 BsaBI GATnn'nnATC Cuts at: 6196 6196 Size: 6202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4009 4391 5026 275 Size: 53 83 186 936 2476 382 635 1451 Fragments arranged by size: 2476 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4635 5033 5307 5454 5865 4635 Size: 398 274 147 411 4972 Fragments arranged by size: 4972 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5977 Fragments arranged by size: 5977 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2199 2552 3420 3458 1186 Size: 1013 353 868 38 3930 Fragments arranged by size: 3930 1013 868 353 38 BseYI C'CCAG_C Cuts at: 2089 2579 2749 2776 5405 5901 2089 Size: 490 170 27 2629 496 2390 Fragments arranged by size: 2629 2390 496 490 170 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2007 2660 1163 Size: 573 271 653 4705 Fragments arranged by size: 4705 653 573 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2404 3054 3652 4413 5441 1540 Size: 275 186 403 650 598 761 1028 2301 Fragments arranged by size: 2301 1028 761 650 598 403 275 186 BsiHKAI G_wGCw'C Cuts at: 2697 3362 3386 4263 4348 5419 2697 Size: 665 24 877 85 1071 3480 Fragments arranged by size: 3480 1071 877 665 85 24 BsmI GAATG_Cn' Cuts at: 2184 3713 3806 2184 Size: 1529 93 4580 Fragments arranged by size: 4580 1529 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2145 2288 2447 2461 2580 2697 Size: 399 264 91 143 159 14 119 117 Cuts at: 2697 2733 2849 3148 3230 3362 3386 3440 3521 Size: 36 116 299 82 132 24 54 81 Cuts at: 3521 4263 4348 5419 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 299 264 159 143 132 119 117 116 91 85 82 81 54 36 24 14 BspEI T'CCGG_A Cuts at: 5865 5865 Size: 6202 BspHI T'CATG_A Cuts at: 3015 4091 5821 3015 Size: 1076 1730 3396 Fragments arranged by size: 3396 1730 1076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2565 2652 2970 3369 3557 844 Size: 1721 87 318 399 188 3489 Fragments arranged by size: 3489 1721 399 318 188 87 BsrBI CCG'CTC Cuts at: 1394 1823 4089 1394 Size: 429 2266 3507 Fragments arranged by size: 3507 2266 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4697 4871 65 Size: 1183 3449 174 1396 Fragments arranged by size: 3449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2304 2403 2981 4846 5033 1700 Size: 110 494 99 578 1865 187 2869 Fragments arranged by size: 2869 1865 578 494 187 110 99 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6202 BssSI C'ACGA_G Cuts at: 2486 4262 5274 2486 Size: 1776 1012 3414 Fragments arranged by size: 3414 1776 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2233 2947 3160 794 Size: 1439 714 213 3836 Fragments arranged by size: 3836 1439 714 213 BstBI TT'CG_AA Cuts at: 5006 5006 Size: 6202 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6202 BstYI r'GATC_y Cuts at: 1972 3532 3899 4284 4301 5753 5839 5851 6197 Size: 1560 367 385 17 1452 86 12 346 Cuts at: 6197 1972 Size: 1977 Fragments arranged by size: 1977 1560 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2622 3330 2622 Size: 708 5494 BtgI C'CryG_G Cuts at: 513 1065 2043 2356 2923 3048 3089 513 Size: 552 978 313 567 125 41 3626 Fragments arranged by size: 3626 978 567 552 313 125 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5463 Fragments arranged by size: 5463 683 56 BtsI GCAGTG_nn' Cuts at: 2976 3068 3718 4512 4532 2976 Size: 92 650 794 20 4646 Fragments arranged by size: 4646 794 650 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2306 2401 Size: 54 1425 50 275 18 198 278 95 Cuts at: 2401 2469 3051 3649 4538 6167 8 Size: 68 582 598 889 1629 43 Fragments arranged by size: 1629 1425 889 598 582 278 275 198 95 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2401 3051 3649 1537 Size: 275 589 650 598 4090 Fragments arranged by size: 4090 650 598 589 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 4132 1353 Size: 854 1925 3423 Fragments arranged by size: 3423 1925 854 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 2154 4789 5163 5309 387 Size: 1064 138 501 64 2635 374 146 1280 Fragments arranged by size: 2635 1280 1064 501 374 146 138 64 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2543 3044 3271 4265 4862 5649 5917 Size: 393 911 501 227 994 597 787 268 Cuts at: 5917 1239 Size: 1524 Fragments arranged by size: 1524 994 911 787 597 501 393 268 227 EcoNI CCTnn'n_nnAGG Cuts at: 2621 3152 3479 2621 Size: 531 327 5344 Fragments arranged by size: 5344 531 327 EcoO109I rG'GnC_Cy Cuts at: 2443 2444 2686 2729 2730 3058 3127 3145 3157 Size: 1 242 43 1 328 69 18 12 Cuts at: 3157 3314 2443 Size: 157 5331 Fragments arranged by size: 5331 328 242 157 69 43 18 12 1 1 EcoRI G'AATT_C Cuts at: 3511 3889 3511 Size: 378 5824 FspI TGC'GCA Cuts at: 4708 4708 Size: 6202 HaeII r_GCGC'y Cuts at: 1678 5349 1678 Size: 3671 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2109 2141 3404 3436 4843 Size: 32 135 32 537 32 1263 32 1407 Cuts at: 4843 4875 4917 4949 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1407 1263 537 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3546 3792 669 Size: 988 281 1608 246 3079 Fragments arranged by size: 3079 1608 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6202 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KpnI G_GTAC'C Cuts at: 3527 3527 Size: 6202 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5316 5500 1305 Size: 4011 184 2007 Fragments arranged by size: 4011 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 10 Size: 54 1425 343 198 4182 Fragments arranged by size: 4182 1425 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2700 4160 4519 4678 518 Size: 648 870 664 1460 359 159 2042 Fragments arranged by size: 2042 1460 870 664 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2233 2742 2864 2947 3091 3122 3210 3618 Size: 627 509 122 83 144 31 88 408 Cuts at: 3618 4295 5443 5688 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 627 509 408 245 144 122 88 83 31 NaeI GCC'GGC Cuts at: 2306 2405 2306 Size: 99 6103 NcoI C'CATG_G Cuts at: 513 1065 2356 513 Size: 552 1291 4359 Fragments arranged by size: 4359 1291 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NgoMIV G'CCGG_C Cuts at: 2304 2403 2304 Size: 99 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 2907 3560 5105 1132 Size: 97 1678 653 1545 2229 Fragments arranged by size: 2229 1678 1545 653 97 PasI CC'CwG_GG Cuts at: 1348 2136 2288 3223 3390 1348 Size: 788 152 935 167 4160 Fragments arranged by size: 4160 935 788 167 152 PciI A'CATG_T Cuts at: 5101 5101 Size: 6202 PflMI CCAn_nnn'nTGG Cuts at: 1108 2689 3294 1108 Size: 1581 605 4016 Fragments arranged by size: 4016 1581 605 PfoI T'CCnGG_A Cuts at: 2802 2892 3270 2802 Size: 90 378 5734 Fragments arranged by size: 5734 378 90 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 5784 5816 4271 Size: 32 1481 32 4657 Fragments arranged by size: 4657 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6202 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PspOMI G'GGCC_C Cuts at: 2443 2457 2576 2729 3144 3226 2443 Size: 14 119 153 415 82 5419 Fragments arranged by size: 5419 415 153 119 82 14 PstI C_TGCA'G Cuts at: 830 2238 2283 2562 2589 2844 3263 3554 830 Size: 1408 45 279 27 255 419 291 3478 Fragments arranged by size: 3478 1408 419 291 279 255 45 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6202 PvuII CAG'CTG Cuts at: 2233 2947 3210 2233 Size: 714 263 5225 Fragments arranged by size: 5225 714 263 SacII CC_GC'GG Cuts at: 3092 3092 Size: 6202 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 4450 6162 4450 Size: 1712 4490 SfcI C'TryA_G Cuts at: 826 1045 2234 2279 2558 2585 2840 3215 3259 Size: 219 1189 45 279 27 255 375 44 Cuts at: 3259 3550 4685 5366 5557 826 Size: 291 1135 681 191 1471 Fragments arranged by size: 1471 1189 1135 681 375 291 279 255 219 191 45 44 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 2735 3529 1872 Size: 863 794 4545 Fragments arranged by size: 4545 863 794 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 4306 5207 5469 820 Size: 197 926 183 2180 901 262 1553 Fragments arranged by size: 2180 1553 926 901 262 197 183 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 2907 3560 1132 Size: 1775 653 3774 Fragments arranged by size: 3774 1775 653 SspI AAT'ATT Cuts at: 5 52 4126 5 Size: 47 4074 2081 Fragments arranged by size: 4074 2081 47 StuI AGG'CCT Cuts at: 1517 2267 2531 2603 1517 Size: 750 264 72 5116 Fragments arranged by size: 5116 750 264 72 StyI C'CwwG_G Cuts at: 513 1065 2175 2356 2391 2448 3310 3641 513 Size: 552 1110 181 35 57 862 331 3074 Fragments arranged by size: 3074 1110 862 552 331 181 57 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2520 2741 3403 4229 4246 4399 4584 Size: 1255 940 221 662 826 17 153 185 Cuts at: 4584 5029 325 Size: 445 1498 Fragments arranged by size: 1498 1255 940 826 662 445 221 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4448 6160 96 Size: 274 80 33 51 1443 2471 1712 138 Fragments arranged by size: 2471 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2754 2786 6162 6194 2754 Size: 32 3376 32 2762 Fragments arranged by size: 3376 2762 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6202 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2111 2722 2896 3397 2111 Size: 611 174 501 4916 Fragments arranged by size: 4916 611 501 174 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 2733 3527 1870 Size: 863 794 4545 Fragments arranged by size: 4545 863 794 XmnI GAAnn'nnTTC Cuts at: 4331 4331 Size: 6202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI