(Circular) (Six-base) MAPSORT of: pFN21AE0954.seq Check: 5837 from: 1 to: 6151 pFN21AE0954 6151 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 2172 4278 4651 1563 Size: 609 2106 373 3063 Fragments arranged by size: 3063 2106 609 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4214 5598 5866 1632 Size: 2582 1384 268 1917 Fragments arranged by size: 2582 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 3170 1166 Size: 2004 4147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 3045 3894 5466 3045 Size: 849 1572 3730 Fragments arranged by size: 3730 1572 849 ApaI G_GGCC'C Cuts at: 2196 2776 2196 Size: 580 5571 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 3460 3686 3838 5947 1493 Size: 1967 226 152 2109 1697 Fragments arranged by size: 2109 1967 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 2594 2892 3258 3476 1870 Size: 73 651 298 366 218 4545 Fragments arranged by size: 4545 651 366 298 218 73 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 2672 2696 3175 3240 Size: 325 217 627 483 402 24 479 65 Cuts at: 3240 3472 4927 618 Size: 232 1455 1842 Fragments arranged by size: 1842 1455 627 483 479 402 325 232 217 65 24 BanII G_rGCy'C Cuts at: 1391 2073 2196 2472 2776 3330 3470 1391 Size: 682 123 276 304 554 140 4072 Fragments arranged by size: 4072 682 554 304 276 140 123 BbeI G_GCGC'C Cuts at: 2676 3179 3244 2676 Size: 503 65 5583 Fragments arranged by size: 5583 503 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5180 BbvCI CC'TCA_GC Cuts at: 3131 3131 Size: 6151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3149 4762 Size: 107 122 71 631 605 337 1140 1613 Cuts at: 4762 136 Size: 1525 Fragments arranged by size: 1613 1525 1140 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 2072 2358 2609 3568 2072 Size: 286 251 959 4655 Fragments arranged by size: 4655 959 286 251 Bme1580I G_kGCm'C Cuts at: 1790 2196 2776 4212 5368 1790 Size: 406 580 1436 1156 2573 Fragments arranged by size: 2573 1436 1156 580 406 BmgBI CAC'GTC Cuts at: 1318 3067 1318 Size: 1749 4402 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3882 4840 442 Size: 416 53 83 399 24 2465 958 1753 Fragments arranged by size: 2465 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3226 3226 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 4811 1239 Size: 1265 2307 2579 Fragments arranged by size: 2579 2307 1265 Bpu10I CC'TnA_GC Cuts at: 3131 3131 Size: 6151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 2573 3263 4276 5141 5439 2468 Size: 105 690 1013 865 298 3180 Fragments arranged by size: 3180 1013 865 690 298 105 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2153 4814 882 Size: 617 654 2661 2219 Fragments arranged by size: 2661 2219 654 617 BsaAI yAC'GTr Cuts at: 493 1204 3172 3942 493 Size: 711 1968 770 2702 Fragments arranged by size: 2702 1968 770 711 BsaBI GATnn'nnATC Cuts at: 6145 6145 Size: 6151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2673 3101 3176 3241 Size: 53 83 186 936 1140 428 75 65 Cuts at: 3241 3439 3958 4340 4975 275 Size: 198 519 382 635 1451 Fragments arranged by size: 1451 1140 936 635 519 428 382 198 186 83 75 65 53 BsaWI w'CCGG_w Cuts at: 2823 4584 4982 5256 5403 5814 2823 Size: 1761 398 274 147 411 3160 Fragments arranged by size: 3160 1761 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3336 3366 1178 Size: 30 165 30 1933 30 3963 Fragments arranged by size: 3963 1933 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3074 1186 Size: 1888 4263 BseYI C'CCAG_C Cuts at: 2221 2927 3308 3314 5354 5850 2221 Size: 706 381 6 2040 496 2522 Fragments arranged by size: 2522 2040 706 496 381 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2990 1163 Size: 573 1254 4324 Fragments arranged by size: 4324 1254 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3601 4362 5390 1540 Size: 275 186 12 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2073 2472 4212 4297 5368 2073 Size: 399 1740 85 1071 2856 Fragments arranged by size: 2856 1740 1071 399 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 BsmBI CGTCTCn'nnnn_ Cuts at: 2027 2027 Size: 6151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2196 2472 2776 3330 3470 4212 Size: 399 283 123 276 304 554 140 742 Cuts at: 4212 4297 5368 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 304 283 276 140 123 85 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3506 844 Size: 2662 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2362 4038 1394 Size: 429 539 1676 3507 Fragments arranged by size: 3507 1676 539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2335 4646 4820 65 Size: 1183 1087 2311 174 1396 Fragments arranged by size: 2311 1396 1183 1087 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3103 4795 4982 1700 Size: 110 1293 1692 187 2869 Fragments arranged by size: 2869 1692 1293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstXI CCAn_nnnn'nTGG Cuts at: 2426 2939 2426 Size: 513 5638 BstYI r'GATC_y Cuts at: 1972 3481 3848 4233 4250 5702 5788 5800 6146 Size: 1509 367 385 17 1452 86 12 346 Cuts at: 6146 1972 Size: 1977 Fragments arranged by size: 1977 1509 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2306 3406 2306 Size: 1100 5051 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 2835 3330 3433 513 Size: 552 939 75 756 495 103 3231 Fragments arranged by size: 3231 939 756 552 495 103 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 2197 2740 2864 3667 4461 4481 2197 Size: 543 124 803 794 20 3867 Fragments arranged by size: 3867 803 794 543 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3455 3455 Size: 6151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3418 5158 809 Size: 507 2102 1740 1802 Fragments arranged by size: 2102 1802 1740 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2737 3598 Size: 54 1425 50 275 18 180 727 861 Cuts at: 3598 4487 6116 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 861 727 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3598 1537 Size: 275 198 1588 4090 Fragments arranged by size: 4090 1588 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2477 4081 1353 Size: 783 341 1604 3423 Fragments arranged by size: 3423 1604 783 341 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4738 5112 5258 387 Size: 1064 138 3149 374 146 1280 Fragments arranged by size: 3149 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2071 2470 2071 Size: 399 5752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 4214 4811 5598 5866 1239 Size: 393 872 1710 597 787 268 1524 Fragments arranged by size: 1710 1524 872 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2309 2309 Size: 6151 EcoO109I rG'GnC_Cy Cuts at: 2193 2584 2772 2773 2896 3042 3262 2193 Size: 391 188 1 123 146 220 5082 Fragments arranged by size: 5082 391 220 188 146 123 1 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2407 3223 2407 Size: 816 5335 FspI TGC'GCA Cuts at: 2327 2732 4657 2327 Size: 405 1925 3821 Fragments arranged by size: 3821 1925 405 HaeII r_GCGC'y Cuts at: 1678 2676 3179 3244 5298 1678 Size: 998 503 65 2054 2531 Fragments arranged by size: 2531 2054 998 503 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2481 2513 4792 4824 4866 Size: 32 135 32 909 32 2279 32 42 Cuts at: 4866 4898 1373 Size: 32 2626 Fragments arranged by size: 2626 2279 909 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KasI G'GCGC_C Cuts at: 2672 3175 3240 2672 Size: 503 65 5583 Fragments arranged by size: 5583 503 65 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5265 5449 1305 Size: 3960 184 2007 Fragments arranged by size: 3960 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2739 10 Size: 54 1425 343 907 3422 Fragments arranged by size: 3422 1425 907 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 4109 4468 4627 518 Size: 648 2004 939 359 159 2042 Fragments arranged by size: 2042 2004 939 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2081 2114 2935 3567 4244 5392 5637 1606 Size: 475 33 821 632 677 1148 245 2120 Fragments arranged by size: 2120 1148 821 677 632 475 245 33 NarI GG'CG_CC Cuts at: 2673 3176 3241 2673 Size: 503 65 5583 Fragments arranged by size: 5583 503 65 NcoI C'CATG_G Cuts at: 513 1065 2004 2835 513 Size: 552 939 831 3829 Fragments arranged by size: 3829 939 831 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2091 2791 3509 5054 1132 Size: 97 862 700 718 1545 2229 Fragments arranged by size: 2229 1545 862 718 700 97 PasI CC'CwG_GG Cuts at: 1348 2548 2566 2951 1348 Size: 1200 18 385 4548 Fragments arranged by size: 4548 1200 385 18 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6151 PfoI T'CCnGG_A Cuts at: 2502 3186 2502 Size: 684 5467 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2584 2896 3262 2584 Size: 312 366 5473 Fragments arranged by size: 5473 366 312 PshAI GACnn'nnGTC Cuts at: 1613 2088 1613 Size: 475 5676 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PspOMI G'GGCC_C Cuts at: 2192 2772 2192 Size: 580 5571 PstI C_TGCA'G Cuts at: 830 3054 3503 830 Size: 2224 449 3478 Fragments arranged by size: 3478 2224 449 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 PvuII CAG'CTG Cuts at: 2114 2114 Size: 6151 SacI G_AGCT'C Cuts at: 2073 2472 2073 Size: 399 5752 SacII CC_GC'GG Cuts at: 2082 2082 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SanDI GG'GwC_CC Cuts at: 3262 3262 Size: 6151 SapI GCTCTTCn'nnn_ Cuts at: 2477 2477 Size: 6151 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 3050 3499 4634 5315 5506 826 Size: 219 2005 449 1135 681 191 1471 Fragments arranged by size: 2005 1471 1135 681 449 219 191 SfoI GGC'GCC Cuts at: 2674 3177 3242 2674 Size: 503 65 5583 Fragments arranged by size: 5583 503 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 3103 1700 Size: 1403 4748 SmaI CCC'GGG Cuts at: 1872 2894 3260 3478 1872 Size: 1022 366 218 4545 Fragments arranged by size: 4545 1022 366 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 2588 3278 4255 5156 5418 Size: 197 926 504 141 690 977 901 262 Cuts at: 5418 820 Size: 1553 Fragments arranged by size: 1553 977 926 901 690 504 262 197 141 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 3152 1517 Size: 1635 4516 StyI C'CwwG_G Cuts at: 513 1065 2004 2334 2835 3590 513 Size: 552 939 330 501 755 3074 Fragments arranged by size: 3074 939 755 552 501 330 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 3250 4178 4195 4348 4533 4978 Size: 1255 1071 599 928 17 153 185 445 Cuts at: 4978 325 Size: 1498 Fragments arranged by size: 1498 1255 1071 928 599 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 4397 6109 Size: 274 80 33 51 1443 729 1691 1712 Cuts at: 6109 96 Size: 138 Fragments arranged by size: 1712 1691 1443 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2929 2961 6111 6143 2929 Size: 32 3150 32 2937 Fragments arranged by size: 3150 2937 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2900 1316 Size: 1584 4567 XbaI T'CTAG_A Cuts at: 2371 3487 2371 Size: 1116 5035 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2707 2707 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 2892 3258 3476 1870 Size: 1022 366 218 4545 Fragments arranged by size: 4545 1022 366 218 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI