(Circular) (Six-base) MAPSORT of: pFN21AE0951.seq Check: 7293 from: 1 to: 6124 pFN21AE0951 6124 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3255 3347 4187 5571 5839 1632 Size: 1623 92 840 1384 268 1917 Fragments arranged by size: 1917 1623 1384 840 268 92 AflII C'TTAA_G Cuts at: 820 1017 2981 820 Size: 197 1964 3963 Fragments arranged by size: 3963 1964 197 AflIII A'CryG_T Cuts at: 1203 2673 5023 6000 1203 Size: 1470 2350 977 1327 Fragments arranged by size: 2350 1470 1327 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 2677 4853 2677 Size: 2176 3948 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AlwNI CAG_nnn'CTG Cuts at: 3867 5439 3867 Size: 1572 4552 ApaI G_GGCC'C Cuts at: 3154 3154 Size: 6124 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 2637 2763 2960 3135 3433 3659 3811 5920 Size: 1144 126 197 175 298 226 152 2109 Cuts at: 5920 1493 Size: 1697 Fragments arranged by size: 2109 1697 1144 298 226 197 175 152 126 AseI AT'TA_AT Cuts at: 160 1032 2843 4678 160 Size: 872 1811 1835 1606 Fragments arranged by size: 1835 1811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 2071 3449 1870 Size: 73 128 1378 4545 Fragments arranged by size: 4545 1378 128 73 BamHI G'GATC_C Cuts at: 2854 3454 3821 2854 Size: 600 367 5157 Fragments arranged by size: 5157 600 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 3253 3445 4900 618 Size: 325 217 627 233 1233 192 1455 1842 Fragments arranged by size: 1842 1455 1233 627 325 233 217 192 BanII G_rGCy'C Cuts at: 1391 3154 3443 1391 Size: 1763 289 4072 Fragments arranged by size: 4072 1763 289 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2606 3335 928 Size: 971 707 729 3717 Fragments arranged by size: 3717 971 729 707 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4315 4349 1903 Size: 34 50 34 2294 34 3678 Fragments arranged by size: 3678 2294 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2929 4016 5226 2929 Size: 1087 1210 3827 Fragments arranged by size: 3827 1210 1087 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 4735 136 Size: 107 122 71 631 605 394 2669 1525 Fragments arranged by size: 2669 1525 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 6119 6119 Size: 6124 BlpI GC'TnA_GC Cuts at: 2129 3180 3541 2129 Size: 1051 361 4712 Fragments arranged by size: 4712 1051 361 Bme1580I G_kGCm'C Cuts at: 1790 2025 3154 3175 3258 4185 5341 1790 Size: 235 1129 21 83 927 1156 2573 Fragments arranged by size: 2573 1156 1129 927 235 83 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3855 4813 442 Size: 416 53 83 399 24 2438 958 1753 Fragments arranged by size: 2438 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3072 1056 Size: 2016 4108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 2511 2873 4784 1239 Size: 1232 40 362 1911 2579 Fragments arranged by size: 2579 1911 1232 362 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4249 5114 5412 4249 Size: 865 298 4961 Fragments arranged by size: 4961 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3069 4787 882 Size: 617 1570 1718 2219 Fragments arranged by size: 2219 1718 1570 617 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 6118 6118 Size: 6124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3931 4313 4948 275 Size: 53 83 186 936 2398 382 635 1451 Fragments arranged by size: 2398 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4557 4955 5229 5376 5787 4557 Size: 398 274 147 411 4894 Fragments arranged by size: 4894 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2774 2804 1178 Size: 30 165 30 1371 30 4498 Fragments arranged by size: 4498 1371 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2388 3317 3332 3353 1186 Size: 1202 929 15 21 3957 Fragments arranged by size: 3957 1202 929 21 15 BseYI C'CCAG_C Cuts at: 2158 5327 5823 2158 Size: 3169 496 2459 Fragments arranged by size: 3169 2459 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3240 1163 Size: 573 1504 4047 Fragments arranged by size: 4047 1504 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2595 4185 4270 5341 2595 Size: 1590 85 1071 3378 Fragments arranged by size: 3378 1590 1071 85 BsmI GAATG_Cn' Cuts at: 3635 3728 3635 Size: 93 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2595 3154 3175 3258 3443 4185 Size: 399 235 570 559 21 83 185 742 Cuts at: 4185 4270 5341 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 559 399 235 185 85 83 21 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 2595 4013 5743 2595 Size: 1418 1730 2976 Fragments arranged by size: 2976 1730 1418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3479 844 Size: 2635 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2942 4011 1394 Size: 429 1119 1069 3507 Fragments arranged by size: 3507 1119 1069 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4619 4793 65 Size: 1183 3371 174 1396 Fragments arranged by size: 3371 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4768 4955 1700 Size: 110 2958 187 2869 Fragments arranged by size: 2958 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2184 2926 96 Size: 2088 742 3294 Fragments arranged by size: 3294 2088 742 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 2053 1184 Size: 869 5255 BstXI CCAn_nnnn'nTGG Cuts at: 2830 3265 2830 Size: 435 5689 BstYI r'GATC_y Cuts at: 1972 2854 3454 3821 4206 4223 5675 5761 5773 Size: 882 600 367 385 17 1452 86 12 Cuts at: 5773 6119 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 882 600 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2664 2664 Size: 6124 BtgI C'CryG_G Cuts at: 513 1065 2897 3040 513 Size: 552 1832 143 3597 Fragments arranged by size: 3597 1832 552 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3159 487 Size: 683 56 1933 3452 Fragments arranged by size: 3452 1933 683 56 BtsI GCAGTG_nn' Cuts at: 3270 3640 4434 4454 3270 Size: 370 794 20 4940 Fragments arranged by size: 4940 794 370 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089 Size: 54 1425 50 275 18 1741 889 1629 Cuts at: 6089 8 Size: 43 Fragments arranged by size: 1741 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2053 3016 3231 4054 1353 Size: 700 963 215 823 3423 Fragments arranged by size: 3423 963 823 700 215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2023 4711 5085 5231 387 Size: 1064 138 434 2688 374 146 1280 Fragments arranged by size: 2688 1280 1064 434 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2471 2511 2873 3255 3347 4187 4784 Size: 393 839 40 362 382 92 840 597 Cuts at: 4784 5571 5839 1239 Size: 787 268 1524 Fragments arranged by size: 1524 840 839 787 597 393 382 362 268 92 40 EcoNI CCTnn'n_nnAGG Cuts at: 3314 3314 Size: 6124 EcoO109I rG'GnC_Cy Cuts at: 2205 3150 3241 3307 2205 Size: 945 91 66 5022 Fragments arranged by size: 5022 945 91 66 EcoRI G'AATT_C Cuts at: 2763 2960 3433 3811 2763 Size: 197 473 378 5076 Fragments arranged by size: 5076 473 378 197 FspI TGC'GCA Cuts at: 2629 4630 2629 Size: 2001 4123 HaeII r_GCGC'y Cuts at: 1678 5271 1678 Size: 3593 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2473 2505 2663 Size: 32 135 32 563 32 306 32 158 Cuts at: 2663 2695 2795 2827 4765 4797 4839 4871 1373 Size: 32 100 32 1938 32 42 32 2626 Fragments arranged by size: 2626 1938 563 306 158 135 100 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3468 3714 669 Size: 988 281 1530 246 3079 Fragments arranged by size: 3079 1530 988 281 246 HindIII A'AGCT_T Cuts at: 748 2978 748 Size: 2230 3894 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2133 2139 5238 5422 1305 Size: 828 6 3099 184 2007 Fragments arranged by size: 3099 2007 828 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4302 Fragments arranged by size: 4302 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2475 4082 4441 4600 518 Size: 648 1309 1607 359 159 2042 Fragments arranged by size: 2042 1607 1309 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2990 3540 4217 5365 5610 1606 Size: 1384 550 677 1148 245 2120 Fragments arranged by size: 2120 1384 1148 677 550 245 NcoI C'CATG_G Cuts at: 513 1065 3040 513 Size: 552 1975 3597 Fragments arranged by size: 3597 1975 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NheI G'CTAG_C Cuts at: 1052 3068 1052 Size: 2016 4108 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 2677 3482 5027 1132 Size: 97 1448 805 1545 2229 Fragments arranged by size: 2229 1545 1448 805 97 PasI CC'CwG_GG Cuts at: 1348 2618 1348 Size: 1270 4854 PciI A'CATG_T Cuts at: 2673 5023 2673 Size: 2350 3774 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PfoI T'CCnGG_A Cuts at: 2091 2448 3051 2091 Size: 357 603 5164 Fragments arranged by size: 5164 603 357 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 2205 Size: 6124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 2309 3694 2309 Size: 1385 4739 PspOMI G'GGCC_C Cuts at: 3150 3150 Size: 6124 PstI C_TGCA'G Cuts at: 830 2431 3476 830 Size: 1601 1045 3478 Fragments arranged by size: 3478 1601 1045 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SanDI GG'GwC_CC Cuts at: 2205 2205 Size: 6124 SapI GCTCTTCn'nnn_ Cuts at: 2053 2053 Size: 6124 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SfcI C'TryA_G Cuts at: 826 1045 2337 2427 2682 3472 4607 5288 5479 Size: 219 1292 90 255 790 1135 681 191 Cuts at: 5479 826 Size: 1471 Fragments arranged by size: 1471 1292 1135 790 681 255 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 2073 3451 1872 Size: 201 1378 4545 Fragments arranged by size: 4545 1378 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 4228 5129 5391 820 Size: 197 926 1038 1247 901 262 1553 Fragments arranged by size: 1553 1247 1038 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3482 1132 Size: 2350 3774 SspI AAT'ATT Cuts at: 5 52 2780 4048 5 Size: 47 2728 1268 2081 Fragments arranged by size: 2728 2081 1268 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6124 StyI C'CwwG_G Cuts at: 513 1065 2823 3040 3258 3563 513 Size: 552 1758 217 218 305 3074 Fragments arranged by size: 3074 1758 552 305 218 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2041 4151 4168 4321 4506 4951 325 Size: 1255 461 2110 17 153 185 445 1498 Fragments arranged by size: 2110 1498 1255 461 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2926 4370 Size: 274 80 33 51 1443 207 742 1444 Cuts at: 4370 6082 96 Size: 1712 138 Fragments arranged by size: 1712 1444 1443 742 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2652 2684 6084 6116 2652 Size: 32 3400 32 2660 Fragments arranged by size: 3400 2660 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2831 1316 Size: 1515 4609 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 2071 3449 1870 Size: 201 1378 4545 Fragments arranged by size: 4545 1378 201 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI