Restriction map for
pFN21AE0946
(Circular) (Six-base) MAPSORT of: pFN21AE0946.seq Check: 9460 from: 1 to: 5806
pFN21AE0946 5806 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2400 2775 3616 278
Size: 53 83 186 936 864 375 841 2468
Fragments arranged by size:
2468 936 864 841 375 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 3050 3149 1937
Size: 325 788 99 4594
Fragments arranged by size:
4594 788 325 99
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 2452 3869 5253 5521 1632
Size: 494 326 1417 1384 268 1917
Fragments arranged by size:
1917 1417 1384 494 326 268
AfeI AGC'GCT
Cuts at: 2483 2483
Size: 5806
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 2923 4535 2923
Size: 1612 4194
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 2811 3549 5121 2811
Size: 738 1572 3496
Fragments arranged by size:
3496 1572 738
ApaI G_GGCC'C
Cuts at: 3075 3103 3075
Size: 28 5778
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 2053 2168 3115 3341 3493 5602 1493
Size: 560 115 947 226 152 2109 1697
Fragments arranged by size:
2109 1697 947 560 226 152 115
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2300 2316 2877 2987 3131 1870
Size: 73 235 122 16 561 110 144 4545
Fragments arranged by size:
4545 561 235 144 122 110 73 16
AvrII C'CTAG_G
Cuts at: 2608 2608
Size: 5806
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3127 4582 618
Size: 325 217 627 1340 1455 1842
Fragments arranged by size:
1842 1455 1340 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2280 2326 3075 3103 3125 1391
Size: 678 211 46 749 28 22 4072
Fragments arranged by size:
4072 749 678 211 46 28 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BbvCI CC'TCA_GC
Cuts at: 2804 2804
Size: 5806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2693 3698 4908 2693
Size: 1005 1210 3591
Fragments arranged by size:
3591 1210 1005
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4417 136
Size: 107 122 71 631 605 2745 1525
Fragments arranged by size:
2745 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2235 5801 2235
Size: 3566 2240
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 3075 3103 3867 5023 1790
Size: 1285 28 764 1156 2573
Fragments arranged by size:
2573 1285 1156 764 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2057 2057
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2623 2960 3072 4466 1239
Size: 1384 337 112 1394 2579
Fragments arranged by size:
2579 1394 1384 337 112
Bpu10I CC'TnA_GC
Cuts at: 2804 2804
Size: 5806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 2399 3931 4796 5094 2009
Size: 390 1532 865 298 2721
Fragments arranged by size:
2721 1532 865 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4469 882
Size: 617 2970 2219
Fragments arranged by size:
2970 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 2126 5800 2126
Size: 3674 2132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2397 2772 3613 3995
Size: 53 83 186 936 864 375 841 382
Cuts at: 3995 4630 275
Size: 635 1451
Fragments arranged by size:
1451 936 864 841 635 382 375 186
83 53
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 2642 2672 1178
Size: 30 165 30 795 30 414 30 4312
Fragments arranged by size:
4312 795 414 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 1186
Size: 1102 4704
BseYI C'CCAG_C
Cuts at: 3038 5009 5505 3038
Size: 1971 496 3339
Fragments arranged by size:
3339 1971 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2137 3256 4017 5045 1540
Size: 275 186 136 1119 761 1028 2301
Fragments arranged by size:
2301 1119 1028 761 275 186 136
BsiHKAI G_wGCw'C
Cuts at: 2280 2697 3867 3952 5023 2280
Size: 417 1170 85 1071 3063
Fragments arranged by size:
3063 1170 1071 417 85
BsmI GAATG_Cn'
Cuts at: 2571 3317 3410 2571
Size: 746 93 4967
Fragments arranged by size:
4967 746 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3086 3086
Size: 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2280 2326 2697 3075 3103 3125
Size: 399 279 211 46 371 378 28 22
Cuts at: 3125 3867 3952 5023 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 371 279 211
85 46 28 22
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3006 4301 4475 65
Size: 1183 1758 1295 174 1396
Fragments arranged by size:
1758 1396 1295 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2778 4450 4637 1700
Size: 110 968 1672 187 2869
Fragments arranged by size:
2869 1672 968 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5806
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 2535 1184
Size: 1351 4455
BstXI CCAn_nnnn'nTGG
Cuts at: 2938 2938
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 2235 2799 2837 3024 3136 3503 3888 3905
Size: 263 564 38 187 112 367 385 17
Cuts at: 3905 5357 5443 5455 5801 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 564 385 367 346 263 187
112 86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2946 2946
Size: 5806
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 2209 2809 3322 4116 4136 2209
Size: 600 513 794 20 3879
Fragments arranged by size:
3879 794 600 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2018 2396 4813 809
Size: 507 702 378 2417 1802
Fragments arranged by size:
2417 1802 702 507 378
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2370 2850
Size: 54 1425 50 275 18 366 174 480
Cuts at: 2850 3253 4142 5771 8
Size: 403 889 1629 43
Fragments arranged by size:
1629 1425 889 480 403 366 275 174
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3736 1353
Size: 2383 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 4767 4913 387
Size: 1064 138 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2278 2278
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2452 2623 2960 3072 3869 4466
Size: 393 494 326 171 337 112 797 597
Cuts at: 4466 5253 5521 1239
Size: 787 268 1524
Fragments arranged by size:
1524 797 787 597 494 393 337 326
268 171 112
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2438 2330
Size: 108 5698
EcoO109I rG'GnC_Cy
Cuts at: 2146 2410 2743 3071 3072 2146
Size: 264 333 328 1 4880
Fragments arranged by size:
4880 333 328 264 1
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2104 2485 4953 1678
Size: 426 381 2468 2531
Fragments arranged by size:
2531 2468 426 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2941 4447 4479 4521
Size: 32 135 32 1337 32 1506 32 42
Cuts at: 4521 4553 1373
Size: 32 2626
Fragments arranged by size:
2626 1506 1337 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2704 2776 3051 3150 3396 669
Size: 988 281 766 72 275 99 246 3079
Fragments arranged by size:
3079 988 766 281 275 246 99 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 2685 3000 4920 5104 1305
Size: 1182 198 315 1920 184 2007
Fragments arranged by size:
2007 1920 1182 315 198 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 2372 2852 10
Size: 54 1425 343 366 174 480 2964
Fragments arranged by size:
2964 1425 480 366 343 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 3764 4123 4282 518
Size: 648 1104 1494 359 159 2042
Fragments arranged by size:
2042 1494 1104 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3222 3899 5047 5292 1606
Size: 1616 677 1148 245 2120
Fragments arranged by size:
2120 1616 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2761 3164 4709 1132
Size: 97 1532 403 1545 2229
Fragments arranged by size:
2229 1545 1532 403 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 1108
Size: 1284 4522
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2743 2410
Size: 333 5473
PshAI GACnn'nnGTC
Cuts at: 1613 2502 1613
Size: 889 4917
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PspOMI G'GGCC_C
Cuts at: 3071 3099 3071
Size: 28 5778
PspXI vC'TCGA_Gb
Cuts at: 2300 2300
Size: 5806
PstI C_TGCA'G
Cuts at: 830 2568 2766 3158 830
Size: 1738 198 392 3478
Fragments arranged by size:
3478 1738 392 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
SacI G_AGCT'C
Cuts at: 2280 2280
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 3049 3148 1936
Size: 1113 99 4594
Fragments arranged by size:
4594 1113 99
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 2381 4054 5766 2381
Size: 1673 1712 2421
Fragments arranged by size:
2421 1712 1673
SfcI C'TryA_G
Cuts at: 826 1045 2564 2762 3154 4289 4970 5161 826
Size: 219 1519 198 392 1135 681 191 1471
Fragments arranged by size:
1519 1471 1135 681 392 219 198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2180 2318 2879 3133 1872
Size: 308 138 561 254 4545
Fragments arranged by size:
4545 561 308 254 138
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 2300 2414 3910 4811 5073
Size: 197 926 81 276 114 1496 901 262
Cuts at: 5073 820
Size: 1553
Fragments arranged by size:
1553 1496 926 901 276 262 197 114
81
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 2004 2367 2586 2608 2826 3245 513
Size: 552 939 363 219 22 218 419 3074
Fragments arranged by size:
3074 939 552 419 363 219 218 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 3850 4003 4188 4633 325
Size: 1255 2253 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 4052 5764
Size: 274 80 33 51 1443 402 1673 1712
Cuts at: 5764 96
Size: 138
Fragments arranged by size:
1712 1673 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 2402 2434 5766 5798 2348
Size: 32 22 32 3332 32 2356
Fragments arranged by size:
3332 2356 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 1316
Size: 1083 4723
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 2300 1943
Size: 357 5449
XmaI C'CCGG_G
Cuts at: 1870 2178 2316 2877 3131 1870
Size: 308 138 561 254 4545
Fragments arranged by size:
4545 561 308 254 138
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2398 2773 3614 276
Size: 53 83 186 936 864 375 841 2468
Fragments arranged by size:
2468 936 864 841 375 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI