(Circular) (Six-base) MAPSORT of: pFN21AE0943.seq Check: 3480 from: 1 to: 5713 pFN21AE0943 5713 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2187 2550 2755 2818 2187 Size: 363 205 63 5082 Fragments arranged by size: 5082 363 205 63 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2940 3523 278 Size: 53 83 186 936 1404 583 2468 Fragments arranged by size: 2468 1404 936 583 186 83 53 AccI GT'mk_AC Cuts at: 1937 3056 1937 Size: 1119 4594 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2821 3776 5160 5428 1632 Size: 1189 955 1384 268 1917 Fragments arranged by size: 1917 1384 1189 955 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5516 AflIII A'CryG_T Cuts at: 1203 4612 5589 1203 Size: 3409 977 1327 Fragments arranged by size: 3409 1327 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 2738 1166 Size: 1572 4141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5713 AlwNI CAG_nnn'CTG Cuts at: 2308 2384 3456 5028 2308 Size: 76 1072 1572 2993 Fragments arranged by size: 2993 1572 1072 76 ApaI G_GGCC'C Cuts at: 2499 2617 2499 Size: 118 5595 ApaLI G'TGCA_C Cuts at: 2132 3770 4926 2132 Size: 1638 1156 2919 Fragments arranged by size: 2919 1638 1156 ApoI r'AATT_y Cuts at: 1493 3022 3248 3400 5509 1493 Size: 1529 226 152 2109 1697 Fragments arranged by size: 2109 1697 1529 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 2356 2498 3038 1870 Size: 73 413 142 540 4545 Fragments arranged by size: 4545 540 413 142 73 BamHI G'GATC_C Cuts at: 3043 3410 3043 Size: 367 5346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 2688 2918 3034 4489 Size: 325 217 627 237 664 230 116 1455 Cuts at: 4489 618 Size: 1842 Fragments arranged by size: 1842 1455 664 627 325 237 230 217 116 BanII G_rGCy'C Cuts at: 1391 2414 2499 2617 3032 1391 Size: 1023 85 118 415 4072 Fragments arranged by size: 4072 1023 415 118 85 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4742 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3904 3938 1903 Size: 34 1967 34 3678 Fragments arranged by size: 3678 1967 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4815 3605 Size: 1210 4503 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2445 4324 136 Size: 107 122 71 631 605 773 1879 1525 Fragments arranged by size: 1879 1525 773 631 605 122 107 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 3130 3130 Size: 5713 Bme1580I G_kGCm'C Cuts at: 1790 2136 2499 2617 3774 4930 1790 Size: 346 363 118 1157 1156 2573 Fragments arranged by size: 2573 1157 1156 363 346 118 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 3444 4402 Size: 416 53 83 399 24 894 1133 958 Cuts at: 4402 442 Size: 1753 Fragments arranged by size: 1753 1133 958 894 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 4373 1239 Size: 960 2174 2579 Fragments arranged by size: 2579 2174 960 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3838 4703 5001 3838 Size: 865 298 4550 Fragments arranged by size: 4550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4376 882 Size: 617 2877 2219 Fragments arranged by size: 2877 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 5707 5707 Size: 5713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2937 3520 3902 4537 Size: 53 83 186 936 1404 583 382 635 Cuts at: 4537 275 Size: 1451 Fragments arranged by size: 1451 1404 936 635 583 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2289 2969 4146 4544 4818 4965 5376 2289 Size: 680 1177 398 274 147 411 2626 Fragments arranged by size: 2626 1177 680 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2402 2894 2924 1178 Size: 30 165 30 969 30 492 30 3967 Fragments arranged by size: 3967 969 492 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5713 BseYI C'CCAG_C Cuts at: 2165 4916 5412 2165 Size: 2751 496 2466 Fragments arranged by size: 2751 2466 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2846 1163 Size: 573 1110 4030 Fragments arranged by size: 4030 1110 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2157 3163 3924 4952 1540 Size: 275 186 156 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 156 BsiHKAI G_wGCw'C Cuts at: 2136 2414 2793 3774 3859 4930 2136 Size: 278 379 981 85 1071 2919 Fragments arranged by size: 2919 1071 981 379 278 85 BsmI GAATG_Cn' Cuts at: 3224 3317 3224 Size: 93 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2414 2499 2617 2793 3032 3774 Size: 399 346 278 85 118 176 239 742 Cuts at: 3774 3859 4930 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 278 239 176 118 85 85 BspEI T'CCGG_A Cuts at: 2289 5376 2289 Size: 3087 2626 BspHI T'CATG_A Cuts at: 3602 5332 3602 Size: 1730 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 2550 2755 2818 3068 844 Size: 1343 363 205 63 250 3489 Fragments arranged by size: 3489 1343 363 250 205 63 BsrBI CCG'CTC Cuts at: 1394 1823 2099 2161 3600 1394 Size: 429 276 62 1439 3507 Fragments arranged by size: 3507 1439 429 276 62 BsrDI GCAATG_nn' Cuts at: 65 1248 2821 4208 4382 65 Size: 1183 1573 1387 174 1396 Fragments arranged by size: 1573 1396 1387 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2220 2227 2322 2508 4357 4544 1700 Size: 110 410 7 95 186 1849 187 2869 Fragments arranged by size: 2869 1849 410 187 186 110 95 7 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 3802 BssHII G'CGCG_C Cuts at: 1925 2117 1925 Size: 192 5521 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 2797 794 Size: 2003 3710 BstBI TT'CG_AA Cuts at: 4517 4517 Size: 5713 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2626 2458 Size: 168 5545 BstYI r'GATC_y Cuts at: 1972 2283 3043 3410 3795 3812 5264 5350 5362 Size: 311 760 367 385 17 1452 86 12 Cuts at: 5362 5708 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 760 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2391 2950 513 Size: 552 1326 559 3276 Fragments arranged by size: 3276 1326 559 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4974 Fragments arranged by size: 4974 683 56 BtsI GCAGTG_nn' Cuts at: 3229 4023 4043 3229 Size: 794 20 4899 Fragments arranged by size: 4899 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5678 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DraIII CAC_nnn'GTG Cuts at: 2319 2319 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2324 2490 Size: 54 1425 50 275 18 324 170 166 Cuts at: 2490 2513 2571 3160 4049 5678 8 Size: 23 58 589 889 1629 43 Fragments arranged by size: 1629 1425 889 589 324 275 170 166 58 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2154 3160 1537 Size: 275 342 1006 4090 Fragments arranged by size: 4090 1006 342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3643 1353 Size: 2290 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4300 4674 4820 387 Size: 1064 138 2711 374 146 1280 Fragments arranged by size: 2711 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2412 2412 Size: 5713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2821 3776 4373 5160 5428 1239 Size: 393 567 622 955 597 787 268 1524 Fragments arranged by size: 1524 955 787 622 597 567 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2183 2183 Size: 5713 EcoO109I rG'GnC_Cy Cuts at: 2447 2472 2564 2581 2614 2724 2910 2447 Size: 25 92 17 33 110 186 5250 Fragments arranged by size: 5250 186 110 92 33 25 17 EcoRI G'AATT_C Cuts at: 3022 3400 3022 Size: 378 5335 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 4860 1678 Size: 3182 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 2745 2777 2894 Size: 32 135 32 800 32 341 32 117 Cuts at: 2894 2926 4354 4386 4428 4460 1373 Size: 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 800 341 135 117 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 4827 5011 1305 Size: 729 2793 184 2007 Fragments arranged by size: 2793 2007 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3891 Fragments arranged by size: 3891 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2694 2738 3671 4030 4189 518 Size: 648 1528 44 933 359 159 2042 Fragments arranged by size: 2042 1528 933 648 359 159 44 MspA1I CmG'CkG Cuts at: 1606 2305 2548 3129 3806 4954 5199 1606 Size: 699 243 581 677 1148 245 2120 Fragments arranged by size: 2120 1148 699 677 581 245 243 NaeI GCC'GGC Cuts at: 2229 2324 2510 2229 Size: 95 186 5432 Fragments arranged by size: 5432 186 95 NcoI C'CATG_G Cuts at: 513 1065 2391 513 Size: 552 1326 3835 Fragments arranged by size: 3835 1326 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NgoMIV G'CCGG_C Cuts at: 2227 2322 2508 2227 Size: 95 186 5432 Fragments arranged by size: 5432 186 95 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 3001 3071 4616 1132 Size: 97 1772 70 1545 2229 Fragments arranged by size: 2229 1772 1545 97 70 PasI CC'CwG_GG Cuts at: 1348 2587 1348 Size: 1239 4474 PciI A'CATG_T Cuts at: 4612 4612 Size: 5713 PflMI CCAn_nnn'nTGG Cuts at: 1108 2397 2610 2665 2750 1108 Size: 1289 213 55 85 4071 Fragments arranged by size: 4071 1289 213 85 55 PfoI T'CCnGG_A Cuts at: 2362 2362 Size: 5713 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2472 2581 2724 2910 2472 Size: 109 143 186 5275 Fragments arranged by size: 5275 186 143 109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PspOMI G'GGCC_C Cuts at: 2495 2613 2495 Size: 118 5595 PstI C_TGCA'G Cuts at: 830 2187 2481 2547 3006 3065 830 Size: 1357 294 66 459 59 3478 Fragments arranged by size: 3478 1357 459 294 66 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 PvuII CAG'CTG Cuts at: 2548 2548 Size: 5713 SacI G_AGCT'C Cuts at: 2414 2414 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SanDI GG'GwC_CC Cuts at: 2472 2724 2910 2472 Size: 252 186 5275 Fragments arranged by size: 5275 252 186 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SexAI A'CCwGG_T Cuts at: 2457 2457 Size: 5713 SfcI C'TryA_G Cuts at: 826 1045 2183 2477 2543 3002 3061 4196 4877 Size: 219 1138 294 66 459 59 1135 681 Cuts at: 4877 5068 826 Size: 191 1471 Fragments arranged by size: 1471 1138 1135 681 459 294 219 191 66 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3817 4718 4980 820 Size: 197 926 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3001 3071 1132 Size: 1869 70 3774 Fragments arranged by size: 3774 1869 70 SspI AAT'ATT Cuts at: 5 52 3637 5 Size: 47 3585 2081 Fragments arranged by size: 3585 2081 47 StuI AGG'CCT Cuts at: 1517 2354 1517 Size: 837 4876 StyI C'CwwG_G Cuts at: 513 1065 2391 3152 513 Size: 552 1326 761 3074 Fragments arranged by size: 3074 1326 761 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2161 2330 2614 2706 3740 3757 3910 Size: 1255 581 169 284 92 1034 17 153 Cuts at: 3910 4095 4540 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1034 581 445 284 185 169 153 92 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3959 5671 Size: 274 80 33 51 1443 30 1952 1712 Cuts at: 5671 96 Size: 138 Fragments arranged by size: 1952 1712 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 3842 3842 Size: 5713 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2938 3521 276 Size: 53 83 186 936 1404 583 2468 Fragments arranged by size: 2468 1404 936 583 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI