(Circular) (Six-base) MAPSORT of: pFN21AE0942.seq Check: 2221 from: 1 to: 5587 pFN21AE0942 5587 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3397 278 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2930 1937 Size: 993 4594 Acc65I G'GTAC_C Cuts at: 2908 2908 Size: 5587 AclI AA'CG_TT Cuts at: 1563 3714 4087 1563 Size: 2151 373 3063 Fragments arranged by size: 3063 2151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3650 5034 5302 1632 Size: 2018 1384 268 1917 Fragments arranged by size: 2018 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5390 AflIII A'CryG_T Cuts at: 1203 2305 4486 5463 1203 Size: 1102 2181 977 1327 Fragments arranged by size: 2181 1327 1102 977 AgeI A'CCGG_T Cuts at: 4418 4418 Size: 5587 AhdI GACnn_n'nnGTC Cuts at: 4316 4316 Size: 5587 AleI CACnn'nnGTG Cuts at: 1166 2870 1166 Size: 1704 3883 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5587 AlwNI CAG_nnn'CTG Cuts at: 2140 2599 3330 4902 2140 Size: 459 731 1572 2825 Fragments arranged by size: 2825 1572 731 459 ApaLI G'TGCA_C Cuts at: 3644 4800 3644 Size: 1156 4431 ApoI r'AATT_y Cuts at: 1493 2896 3122 3274 5383 1493 Size: 1403 226 152 2109 1697 Fragments arranged by size: 2109 1697 1403 226 152 AseI AT'TA_AT Cuts at: 160 1032 4141 160 Size: 872 3109 1606 Fragments arranged by size: 3109 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 AvaI C'yCGr_G Cuts at: 1870 1943 2348 2912 1870 Size: 73 405 564 4545 Fragments arranged by size: 4545 564 405 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2623 2656 2623 Size: 33 5554 BamHI G'GATC_C Cuts at: 2917 3284 2917 Size: 367 5220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2114 2908 4363 618 Size: 325 217 627 241 86 794 1455 1842 Fragments arranged by size: 1842 1455 794 627 325 241 217 86 BanII G_rGCy'C Cuts at: 1391 2225 2280 2529 2906 1391 Size: 834 55 249 377 4072 Fragments arranged by size: 4072 834 377 249 55 BbeI G_GCGC'C Cuts at: 2118 2118 Size: 5587 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2673 928 Size: 971 774 3842 Fragments arranged by size: 3842 971 774 BbvCI CC'TCA_GC Cuts at: 2730 2730 Size: 5587 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3778 3812 1903 Size: 34 1841 34 3678 Fragments arranged by size: 3678 1841 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2651 3479 4689 2651 Size: 828 1210 3549 Fragments arranged by size: 3549 1210 828 BclI T'GATC_A Cuts at: 1548 1548 Size: 5587 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4198 136 Size: 107 122 71 631 605 2526 1525 Fragments arranged by size: 2526 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5582 5582 Size: 5587 BlpI GC'TnA_GC Cuts at: 2383 3004 2383 Size: 621 4966 Bme1580I G_kGCm'C Cuts at: 1790 2476 3648 4804 1790 Size: 686 1172 1156 2573 Fragments arranged by size: 2573 1172 1156 686 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2228 2755 3318 Size: 416 53 83 399 24 811 527 563 Cuts at: 3318 4276 442 Size: 958 1753 Fragments arranged by size: 1753 958 811 563 527 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2582 4247 1239 Size: 797 546 1665 2579 Fragments arranged by size: 2579 1665 797 546 Bpu10I CC'TnA_GC Cuts at: 2177 2730 2177 Size: 553 5034 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3712 4577 4875 3712 Size: 865 298 4424 Fragments arranged by size: 4424 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 4250 882 Size: 617 1041 1710 2219 Fragments arranged by size: 2219 1710 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 2306 3378 493 Size: 711 1102 1072 2702 Fragments arranged by size: 2702 1102 1072 711 BsaBI GATnn'nnATC Cuts at: 5581 5581 Size: 5587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 2318 3394 3776 Size: 53 83 186 936 582 203 1076 382 Cuts at: 3776 4411 275 Size: 635 1451 Fragments arranged by size: 1451 1076 936 635 582 382 203 186 83 53 BsaWI w'CCGG_w Cuts at: 2583 4020 4418 4692 4839 5250 2583 Size: 1437 398 274 147 411 2920 Fragments arranged by size: 2920 1437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2306 2336 1178 Size: 30 165 30 903 30 4429 Fragments arranged by size: 4429 903 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2327 1186 Size: 997 144 4446 Fragments arranged by size: 4446 997 144 BseYI C'CCAG_C Cuts at: 2110 2556 4790 5286 2110 Size: 446 2234 496 2411 Fragments arranged by size: 2411 2234 496 446 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2346 2558 1163 Size: 573 610 212 4192 Fragments arranged by size: 4192 610 573 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3798 4826 1540 Size: 275 186 1036 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2280 2529 3648 3733 4804 2280 Size: 249 1119 85 1071 3063 Fragments arranged by size: 3063 1119 1071 249 85 BsmI GAATG_Cn' Cuts at: 3098 3191 3098 Size: 93 5494 BsmBI CGTCTCn'nnnn_ Cuts at: 2309 2309 Size: 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2225 2280 2476 2529 2906 3648 3733 Size: 399 435 55 196 53 377 742 85 Cuts at: 3733 4804 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 377 196 85 55 53 BspEI T'CCGG_A Cuts at: 5250 5250 Size: 5587 BspHI T'CATG_A Cuts at: 3476 5206 3476 Size: 1730 3857 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2942 844 Size: 2098 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2020 2589 3474 1394 Size: 429 197 569 885 3507 Fragments arranged by size: 3507 885 569 429 197 BsrDI GCAATG_nn' Cuts at: 65 1248 4082 4256 65 Size: 1183 2834 174 1396 Fragments arranged by size: 2834 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4231 4418 1700 Size: 110 2421 187 2869 Fragments arranged by size: 2869 2421 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5587 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5587 BssSI C'ACGA_G Cuts at: 3647 4659 3647 Size: 1012 4575 BstAPI GCAn_nnn'nTGC Cuts at: 794 2260 2340 2599 794 Size: 1466 80 259 3782 Fragments arranged by size: 3782 1466 259 80 BstBI TT'CG_AA Cuts at: 4391 4391 Size: 5587 BstEII G'GTnAC_C Cuts at: 1184 2779 1184 Size: 1595 3992 BstYI r'GATC_y Cuts at: 1972 2917 3284 3669 3686 5138 5224 5236 5582 Size: 945 367 385 17 1452 86 12 346 Cuts at: 5582 1972 Size: 1977 Fragments arranged by size: 1977 1452 945 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2610 2695 513 Size: 552 939 606 85 3405 Fragments arranged by size: 3405 939 606 552 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4848 Fragments arranged by size: 4848 683 56 BtsI GCAGTG_nn' Cuts at: 2483 3103 3897 3917 2483 Size: 620 794 20 4153 Fragments arranged by size: 4153 794 620 20 ClaI AT'CG_AT Cuts at: 1554 2161 1554 Size: 607 4980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5552 DraI TTT'AAA Cuts at: 2891 2891 Size: 5587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2507 4594 809 Size: 507 1191 2087 1802 Fragments arranged by size: 2087 1802 1191 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 2681 3034 Size: 54 1425 50 275 18 528 323 353 Cuts at: 3034 3923 5552 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 528 353 323 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3034 1537 Size: 275 1222 4090 Fragments arranged by size: 4090 1222 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3517 1353 Size: 2164 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2234 4174 4548 4694 387 Size: 1064 138 645 1940 374 146 1280 Fragments arranged by size: 1940 1280 1064 645 374 146 138 EcoICRI GAG'CTC Cuts at: 2278 2527 2278 Size: 249 5338 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2582 3650 4247 5034 5302 1239 Size: 393 404 546 1068 597 787 268 1524 Fragments arranged by size: 1524 1068 787 597 546 404 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2729 2729 Size: 5587 EcoRI G'AATT_C Cuts at: 2896 3274 2896 Size: 378 5209 FspI TGC'GCA Cuts at: 4093 4093 Size: 5587 HaeII r_GCGC'y Cuts at: 1678 2118 4734 1678 Size: 440 2616 2531 Fragments arranged by size: 2616 2531 440 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2154 2186 4228 4260 4302 Size: 32 135 32 582 32 2042 32 42 Cuts at: 4302 4334 1373 Size: 32 2626 Fragments arranged by size: 2626 2042 582 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2512 2931 3177 669 Size: 988 281 574 419 246 3079 Fragments arranged by size: 3079 988 574 419 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5587 HpaI GTT'AAC Cuts at: 3177 3177 Size: 5587 KasI G'GCGC_C Cuts at: 2114 2114 Size: 5587 KpnI G_GTAC'C Cuts at: 2912 2912 Size: 5587 MfeI C'AATT_G Cuts at: 3186 3186 Size: 5587 MluI A'CGCG_T Cuts at: 5463 5463 Size: 5587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4701 4885 1305 Size: 3396 184 2007 Fragments arranged by size: 3396 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2683 10 Size: 54 1425 343 851 2914 Fragments arranged by size: 2914 1425 851 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2870 3545 3904 4063 518 Size: 648 1704 675 359 159 2042 Fragments arranged by size: 2042 1704 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2560 2676 2812 3003 3680 4828 5073 Size: 531 423 116 136 191 677 1148 245 Cuts at: 5073 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 531 423 245 191 136 116 NarI GG'CG_CC Cuts at: 2115 2115 Size: 5587 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4096 Fragments arranged by size: 4096 939 552 NdeI CA'TA_TG Cuts at: 387 2869 387 Size: 2482 3105 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5587 NotI GC'GGCC_GC Cuts at: 3034 3034 Size: 5587 NspI r_CATG'y Cuts at: 1132 1229 2945 4490 1132 Size: 97 1716 1545 2229 Fragments arranged by size: 2229 1716 1545 97 PasI CC'CwG_GG Cuts at: 1348 2389 1348 Size: 1041 4546 PciI A'CATG_T Cuts at: 4486 4486 Size: 5587 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 1108 Size: 1095 4492 PmeI GTTT'AAAC Cuts at: 2891 2891 Size: 5587 PmlI CAC'GTG Cuts at: 2306 2306 Size: 5587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 5169 5201 3656 Size: 32 1481 32 4042 Fragments arranged by size: 4042 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5587 PsiI TTA'TAA Cuts at: 3157 3157 Size: 5587 PspXI vC'TCGA_Gb Cuts at: 2348 2348 Size: 5587 PstI C_TGCA'G Cuts at: 830 2046 2604 2939 830 Size: 1216 558 335 3478 Fragments arranged by size: 3478 1216 558 335 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5587 PvuII CAG'CTG Cuts at: 2137 2560 2812 2137 Size: 423 252 4912 Fragments arranged by size: 4912 423 252 SacI G_AGCT'C Cuts at: 2280 2529 2280 Size: 249 5338 SalI G'TCGA_C Cuts at: 1936 2929 1936 Size: 993 4594 SbfI CC_TGCA'GG Cuts at: 2939 2939 Size: 5587 ScaI AGT'ACT Cuts at: 3835 5547 3835 Size: 1712 3875 SfcI C'TryA_G Cuts at: 826 1045 2042 2121 2437 2600 2935 4070 4751 Size: 219 997 79 316 163 335 1135 681 Cuts at: 4751 4942 826 Size: 191 1471 Fragments arranged by size: 1471 1135 997 681 335 316 219 191 163 79 SfoI GGC'GCC Cuts at: 2116 2116 Size: 5587 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5587 SmaI CCC'GGG Cuts at: 1872 2914 1872 Size: 1042 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 3691 4592 4854 820 Size: 197 926 405 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 405 262 197 SnaBI TAC'GTA Cuts at: 493 3378 493 Size: 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5587 SphI G_CATG'C Cuts at: 1132 2945 1132 Size: 1813 3774 SspI AAT'ATT Cuts at: 5 52 3511 5 Size: 47 3459 2081 Fragments arranged by size: 3459 2081 47 StuI AGG'CCT Cuts at: 1517 2879 1517 Size: 1362 4225 StyI C'CwwG_G Cuts at: 513 1065 2004 3026 513 Size: 552 939 1022 3074 Fragments arranged by size: 3074 1022 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3614 3631 3784 3969 4414 325 Size: 1255 2034 17 153 185 445 1498 Fragments arranged by size: 2034 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3833 5545 96 Size: 274 80 33 51 1443 1856 1712 138 Fragments arranged by size: 1856 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 5547 5579 2306 Size: 32 3209 32 2314 Fragments arranged by size: 3209 2314 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5587 XbaI T'CTAG_A Cuts at: 2923 2923 Size: 5587 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2107 2011 Size: 96 5491 XhoI C'TCGA_G Cuts at: 1943 2348 1943 Size: 405 5182 XmaI C'CCGG_G Cuts at: 1870 2912 1870 Size: 1042 4545 XmnI GAAnn'nnTTC Cuts at: 3716 3716 Size: 5587 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3395 276 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI