Restriction map for
pFN21AE0941
(Circular) (Six-base) MAPSORT of: pFN21AE0941.seq Check: 1868 from: 1 to: 5509
pFN21AE0941 5509 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2852 1937
Size: 915 4594
Acc65I G'GTAC_C
Cuts at: 2031 2830 2031
Size: 799 4710
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 3572 4956 5224 1632
Size: 833 1107 1384 268 1917
Fragments arranged by size:
1917 1384 1107 833 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 4408 5385 1203
Size: 3205 977 1327
Fragments arranged by size:
3205 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 2725 3252 4824 2725
Size: 527 1572 3410
Fragments arranged by size:
3410 1572 527
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2818 3044 3196 5305 1493
Size: 1325 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 2537 2834 1870
Size: 73 229 365 297 4545
Fragments arranged by size:
4545 365 297 229 73
BamHI G'GATC_C
Cuts at: 2839 3206 2839
Size: 367 5142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2830 4285 618
Size: 325 217 627 244 799 1455 1842
Fragments arranged by size:
1842 1455 799 627 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2181 2274 2828 1391
Size: 790 93 554 4072
Fragments arranged by size:
4072 790 554 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4538
BbvCI CC'TCA_GC
Cuts at: 2277 2277
Size: 5509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3700 3734 1903
Size: 34 1763 34 3678
Fragments arranged by size:
3678 1763 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4120 136
Size: 107 122 71 631 605 2448 1525
Fragments arranged by size:
2448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5504 5504
Size: 5509
BlpI GC'TnA_GC
Cuts at: 2498 2926 2498
Size: 428 5081
Bme1580I G_kGCm'C
Cuts at: 1790 3570 4726 1790
Size: 1780 1156 2573
Fragments arranged by size:
2573 1780 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3240 4198 442
Size: 416 53 83 399 24 1823 958 1753
Fragments arranged by size:
1823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2801 1056
Size: 1745 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2605 4169 1239
Size: 1016 350 1564 2579
Fragments arranged by size:
2579 1564 1016 350
Bpu10I CC'TnA_GC
Cuts at: 2277 2277
Size: 5509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 3634 4499 4797 2714
Size: 920 865 298 3426
Fragments arranged by size:
3426 920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3316 3698 4333 275
Size: 53 83 186 936 1783 382 635 1451
Fragments arranged by size:
1783 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2274 2304 2578 2608 1178
Size: 30 165 30 871 30 274 30 4079
Fragments arranged by size:
4079 871 274 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5509
BseYI C'CCAG_C
Cuts at: 2038 4712 5208 2038
Size: 2674 496 2339
Fragments arranged by size:
2674 2339 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4936
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2712 2959 3720 4748 1540
Size: 275 186 711 247 761 1028 2301
Fragments arranged by size:
2301 1028 761 711 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2274 3570 3655 4726 2181
Size: 93 1296 85 1071 2964
Fragments arranged by size:
2964 1296 1071 93 85
BsmI GAATG_Cn'
Cuts at: 2553 3020 3113 2553
Size: 467 93 4949
Fragments arranged by size:
4949 467 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2274 2828 3570 3655 4726 1391
Size: 399 391 93 554 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 399 391 93 85
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2733 2864 844
Size: 1889 131 3489
Fragments arranged by size:
3489 1889 131
BsrBI CCG'CTC
Cuts at: 1394 1823 3396 1394
Size: 429 1573 3507
Fragments arranged by size:
3507 1573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 4178 65
Size: 1183 2756 174 1396
Fragments arranged by size:
2756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4153 4340 1700
Size: 110 2343 187 2869
Fragments arranged by size:
2869 2343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5509
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstXI CCAn_nnnn'nTGG
Cuts at: 2387 2387
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2839 3206 3591 3608 5060 5146 5158 5504
Size: 867 367 385 17 1452 86 12 346
Cuts at: 5504 1972
Size: 1977
Fragments arranged by size:
1977 1452 867 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2380 2792 513
Size: 552 939 376 412 3230
Fragments arranged by size:
3230 939 552 412 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4770
Fragments arranged by size:
4770 683 56
BtsI GCAGTG_nn'
Cuts at: 2318 3025 3819 3839 2318
Size: 707 794 20 3988
Fragments arranged by size:
3988 794 707 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2709 2956 3845
Size: 54 1425 50 275 18 879 247 889
Cuts at: 3845 5474 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 879 275 247 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2709 2956 1537
Size: 275 897 247 4090
Fragments arranged by size:
4090 897 275 247
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2351 3439 1353
Size: 789 209 1088 3423
Fragments arranged by size:
3423 1088 789 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 4470 4616 387
Size: 1064 138 2507 374 146 1280
Fragments arranged by size:
2507 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 2272 2179
Size: 93 5416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2465 2605 3572 4169 4956 5224
Size: 393 623 210 140 967 597 787 268
Cuts at: 5224 1239
Size: 1524
Fragments arranged by size:
1524 967 787 623 597 393 268 210
140
EcoO109I rG'GnC_Cy
Cuts at: 2061 2341 2404 2722 2061
Size: 280 63 318 4848
Fragments arranged by size:
4848 318 280 63
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 5509
FspI TGC'GCA
Cuts at: 4015 4015
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 4656 1678
Size: 2978 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2274 2306 4150 4182 4224
Size: 32 135 32 702 32 1844 32 42
Cuts at: 4224 4256 1373
Size: 32 2626
Fragments arranged by size:
2626 1844 702 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3099 669
Size: 988 281 915 246 3079
Fragments arranged by size:
3079 988 915 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KpnI G_GTAC'C
Cuts at: 2035 2834 2035
Size: 799 4710
MfeI C'AATT_G
Cuts at: 2769 3108 2769
Size: 339 5170
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4623 4807 1305
Size: 3318 184 2007
Fragments arranged by size:
3318 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3687
Fragments arranged by size:
3687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2385 2594 3467 3826 3985 518
Size: 648 1053 166 209 873 359 159 2042
Fragments arranged by size:
2042 1053 873 648 359 209 166 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2925 3602 4750 4995 1606
Size: 432 887 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 432 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2380 2792 513
Size: 552 939 376 412 3230
Fragments arranged by size:
3230 939 552 412 376
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 2797 1052
Size: 1745 3764
NotI GC'GGCC_GC
Cuts at: 2956 2956
Size: 5509
NspI r_CATG'y
Cuts at: 1132 1229 2867 4412 1132
Size: 97 1638 1545 2229
Fragments arranged by size:
2229 1638 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PfoI T'CCnGG_A
Cuts at: 2739 2739
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2061 2404 2722 2061
Size: 343 318 4848
Fragments arranged by size:
4848 343 318
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2550 2861 830
Size: 1720 311 3478
Fragments arranged by size:
3478 1720 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
PvuII CAG'CTG
Cuts at: 2038 2038
Size: 5509
SacI G_AGCT'C
Cuts at: 2181 2274 2181
Size: 93 5416
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2546 2857 3992 4673 4864 826
Size: 219 1501 311 1135 681 191 1471
Fragments arranged by size:
1501 1471 1135 681 311 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2836 1872
Size: 964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3613 4514 4776 820
Size: 197 926 786 884 901 262 1553
Fragments arranged by size:
1553 926 901 884 786 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5509
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2380 2469 2792 2948 513
Size: 552 939 333 43 89 323 156 3074
Fragments arranged by size:
3074 939 552 333 323 156 89 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 3536 3553 3706 3891 4336 325
Size: 1255 1002 954 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1002 954 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2519 3755 5467
Size: 274 80 33 51 1443 542 1236 1712
Cuts at: 5467 96
Size: 138
Fragments arranged by size:
1712 1443 1236 542 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2578 2610 5469 5501 2578
Size: 32 2859 32 2586
Fragments arranged by size:
2859 2586 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2834 1870
Size: 964 4545
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI