(Circular) (Six-base) MAPSORT of: pFN21AE0932.seq Check: 4509 from: 1 to: 5938 pFN21AE0932 5938 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3009 3281 1937 Size: 1072 272 4594 Fragments arranged by size: 4594 1072 272 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4001 5385 5653 1632 Size: 2369 1384 268 1917 Fragments arranged by size: 2369 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 2570 3681 5253 2570 Size: 1111 1572 3255 Fragments arranged by size: 3255 1572 1111 ApaI G_GGCC'C Cuts at: 2801 2801 Size: 5938 ApaLI G'TGCA_C Cuts at: 2678 3995 5151 2678 Size: 1317 1156 3465 Fragments arranged by size: 3465 1317 1156 ApoI r'AATT_y Cuts at: 1493 2618 2753 2825 3247 3473 3625 5734 1493 Size: 1125 135 72 422 226 152 2109 1697 Fragments arranged by size: 2109 1697 1125 422 226 152 135 72 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2633 3263 1870 Size: 73 690 630 4545 Fragments arranged by size: 4545 690 630 73 BamHI G'GATC_C Cuts at: 2459 3268 3635 2459 Size: 809 367 4762 Fragments arranged by size: 4762 809 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2416 3259 4714 618 Size: 325 217 627 629 843 1455 1842 Fragments arranged by size: 1842 1455 843 629 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2801 3257 1391 Size: 678 732 456 4072 Fragments arranged by size: 4072 732 678 456 BbeI G_GCGC'C Cuts at: 2420 2420 Size: 5938 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4967 BbvCI CC'TCA_GC Cuts at: 2338 2338 Size: 5938 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3136 3830 5040 3136 Size: 694 1210 4034 Fragments arranged by size: 4034 1210 694 BclI T'GATC_A Cuts at: 1548 3045 1548 Size: 1497 4441 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 2682 2801 3999 5155 1790 Size: 892 119 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 892 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3669 4627 442 Size: 416 53 83 399 24 2252 958 1753 Fragments arranged by size: 2252 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 4598 1239 Size: 844 2515 2579 Fragments arranged by size: 2579 2515 844 Bpu10I CC'TnA_GC Cuts at: 2338 2338 Size: 5938 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2640 4601 882 Size: 617 1141 1961 2219 Fragments arranged by size: 2219 1961 1141 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2417 3745 4127 4762 Size: 53 83 186 936 884 1328 382 635 Cuts at: 4762 275 Size: 1451 Fragments arranged by size: 1451 1328 936 884 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5713 Fragments arranged by size: 5713 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2137 1186 Size: 951 4987 BseYI C'CCAG_C Cuts at: 2377 2554 2864 5141 5637 2377 Size: 177 310 2277 496 2678 Fragments arranged by size: 2678 2277 496 310 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 1163 Size: 573 771 4594 Fragments arranged by size: 4594 771 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2682 3208 3999 4084 5155 2682 Size: 526 791 85 1071 3465 Fragments arranged by size: 3465 1071 791 526 85 BsmI GAATG_Cn' Cuts at: 3449 3542 3449 Size: 93 5845 BsmBI CGTCTCn'nnnn_ Cuts at: 2356 2966 2356 Size: 610 5328 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2682 2801 3208 3257 3999 4084 Size: 399 279 613 119 407 49 742 85 Cuts at: 4084 5155 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 613 407 399 279 119 85 49 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3293 844 Size: 2449 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3024 3825 1394 Size: 429 1201 801 3507 Fragments arranged by size: 3507 1201 801 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2714 4433 4607 65 Size: 1183 1466 1719 174 1396 Fragments arranged by size: 1719 1466 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 2265 3998 5010 2265 Size: 1733 1012 3193 Fragments arranged by size: 3193 1733 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstYI r'GATC_y Cuts at: 1972 2459 3268 3635 4020 4037 5489 5575 5587 Size: 487 809 367 385 17 1452 86 12 Cuts at: 5587 5933 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 809 487 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3010 3010 Size: 5938 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4447 Fragments arranged by size: 4447 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 2358 3454 4248 4268 2358 Size: 1096 794 20 4028 Fragments arranged by size: 4028 1096 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 2841 3242 2841 Size: 401 5537 DraIII CAC_nnn'GTG Cuts at: 2117 2117 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 2355 2429 Size: 54 1425 50 275 18 498 27 74 Cuts at: 2429 2998 3385 4274 5903 8 Size: 569 387 889 1629 43 Fragments arranged by size: 1629 1425 889 569 498 387 275 74 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 3868 1353 Size: 1325 1190 3423 Fragments arranged by size: 3423 1325 1190 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4525 4899 5045 387 Size: 1064 138 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 4001 4598 5385 5653 1239 Size: 393 451 1918 597 787 268 1524 Fragments arranged by size: 1918 1524 787 597 451 393 268 EcoO109I rG'GnC_Cy Cuts at: 2095 2311 2797 2798 2095 Size: 216 486 1 5235 Fragments arranged by size: 5235 486 216 1 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 2420 5085 1678 Size: 742 2665 2531 Fragments arranged by size: 2665 2531 742 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2531 2563 2639 2671 4579 Size: 32 135 32 959 32 76 32 1908 Cuts at: 4579 4611 4653 4685 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1908 959 135 76 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 2083 2603 748 Size: 1335 520 4083 Fragments arranged by size: 4083 1335 520 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KasI G'GCGC_C Cuts at: 2416 2416 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 2037 3537 2037 Size: 1500 4438 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5052 5236 1305 Size: 3747 184 2007 Fragments arranged by size: 3747 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4116 Fragments arranged by size: 4116 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3896 4255 4414 518 Size: 648 2730 359 159 2042 Fragments arranged by size: 2730 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2513 3354 4031 5179 5424 1606 Size: 907 841 677 1148 245 2120 Fragments arranged by size: 2120 1148 907 841 677 245 NarI GG'CG_CC Cuts at: 2417 2417 Size: 5938 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4447 Fragments arranged by size: 4447 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2693 3296 4841 1132 Size: 97 1464 603 1545 2229 Fragments arranged by size: 2229 1545 1464 603 97 PasI CC'CwG_GG Cuts at: 1348 2411 1348 Size: 1063 4875 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 2117 1108 Size: 1009 4929 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2736 1613 Size: 1123 4815 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PspOMI G'GGCC_C Cuts at: 2797 2797 Size: 5938 PstI C_TGCA'G Cuts at: 830 2029 2319 3290 830 Size: 1199 290 971 3478 Fragments arranged by size: 3478 1199 971 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SfcI C'TryA_G Cuts at: 826 1045 2025 2315 3286 4421 5102 5293 826 Size: 219 980 290 971 1135 681 191 1471 Fragments arranged by size: 1471 1135 980 971 681 290 219 191 SfoI GGC'GCC Cuts at: 2418 2418 Size: 5938 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2633 4042 4943 5205 820 Size: 197 926 690 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 690 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 2693 3296 1132 Size: 1561 603 3774 Fragments arranged by size: 3774 1561 603 SspI AAT'ATT Cuts at: 5 52 2851 3862 5 Size: 47 2799 1011 2081 Fragments arranged by size: 2799 2081 1011 47 StuI AGG'CCT Cuts at: 1517 2495 1517 Size: 978 4960 StyI C'CwwG_G Cuts at: 513 1065 2004 3377 513 Size: 552 939 1373 3074 Fragments arranged by size: 3074 1373 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3965 3982 4135 4320 4765 325 Size: 1255 2385 17 153 185 445 1498 Fragments arranged by size: 2385 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4184 5896 96 Size: 274 80 33 51 1443 2207 1712 138 Fragments arranged by size: 2207 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2251 2283 5898 5930 2251 Size: 32 3615 32 2259 Fragments arranged by size: 3615 2259 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2608 2608 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 2633 1943 Size: 690 5248 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 4067 4067 Size: 5938 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI