(Circular) (Six-base) MAPSORT of: pFN21AE0925.seq Check: 4399 from: 1 to: 5689 pFN21AE0925 5689 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2204 3432 5004 2204 Size: 1228 1572 2889 Fragments arranged by size: 2889 1572 1228 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2128 2351 2453 2649 2998 3224 3376 5485 Size: 635 223 102 196 349 226 152 2109 Cuts at: 5485 1493 Size: 1697 Fragments arranged by size: 2109 1697 635 349 226 223 196 152 102 AseI AT'TA_AT Cuts at: 160 1032 2256 4243 160 Size: 872 1224 1987 1606 Fragments arranged by size: 1987 1606 1224 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2048 3014 1870 Size: 73 105 966 4545 Fragments arranged by size: 4545 966 105 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2618 2651 2618 Size: 33 5656 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 3010 4465 618 Size: 325 217 627 241 982 1455 1842 Fragments arranged by size: 1842 1455 982 627 325 241 217 BanII G_rGCy'C Cuts at: 1391 2067 2095 3008 1391 Size: 676 28 913 4072 Fragments arranged by size: 4072 913 676 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2047 2471 928 Size: 971 148 424 4146 Fragments arranged by size: 4146 971 424 148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2619 2740 3581 4791 2619 Size: 121 841 1210 3517 Fragments arranged by size: 3517 1210 841 121 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 2244 3106 2244 Size: 862 4827 Bme1580I G_kGCm'C Cuts at: 1790 3750 4906 1790 Size: 1960 1156 2573 Fragments arranged by size: 2573 1960 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4349 1239 Size: 3110 2579 Bpu10I CC'TnA_GC Cuts at: 2976 2976 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2259 3814 4679 4977 2259 Size: 1555 865 298 2971 Fragments arranged by size: 2971 1555 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 2751 5683 2751 Size: 2932 2757 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2082 2112 1178 Size: 30 165 30 679 30 4755 Fragments arranged by size: 4755 679 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2103 1186 Size: 917 4772 BseYI C'CCAG_C Cuts at: 4892 5388 4892 Size: 496 5193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2619 1163 Size: 573 883 4233 Fragments arranged by size: 4233 883 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 3750 3835 4906 2067 Size: 1683 85 1071 2850 Fragments arranged by size: 2850 1683 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2095 3008 3750 3835 4906 1391 Size: 399 277 28 913 742 85 1071 2174 Fragments arranged by size: 2174 1071 913 742 399 277 85 28 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2239 3044 844 Size: 1395 805 3489 Fragments arranged by size: 3489 1395 805 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2020 4184 4358 65 Size: 1183 772 2164 174 1396 Fragments arranged by size: 2164 1396 1183 772 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2742 513 Size: 552 1677 3460 Fragments arranged by size: 3460 1677 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2347 2993 2347 Size: 646 5043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2051 4696 809 Size: 507 735 2645 1802 Fragments arranged by size: 2645 1802 735 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2667 3136 4025 Size: 54 1425 50 275 18 837 469 889 Cuts at: 4025 5654 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 837 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2065 2065 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3752 4349 5136 5404 1239 Size: 393 2120 597 787 268 1524 Fragments arranged by size: 2120 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 4330 4362 4404 Size: 32 135 32 749 32 1977 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1977 749 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 10 Size: 54 1425 343 837 3030 Fragments arranged by size: 3030 1425 837 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 3647 4006 4165 518 Size: 648 1147 1334 359 159 2042 Fragments arranged by size: 2042 1334 1147 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3105 3782 4930 5175 1606 Size: 1499 677 1148 245 2120 Fragments arranged by size: 2120 1499 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2742 513 Size: 552 1677 3460 Fragments arranged by size: 3460 1677 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2577 3047 4592 1132 Size: 97 1348 470 1545 2229 Fragments arranged by size: 2229 1545 1348 470 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2153 1108 Size: 1045 4644 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SacI G_AGCT'C Cuts at: 2067 2067 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 3037 4172 4853 5044 826 Size: 219 1992 1135 681 191 1471 Fragments arranged by size: 1992 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2050 3016 1872 Size: 178 966 4545 Fragments arranged by size: 4545 966 178 SmlI C'TyrA_G Cuts at: 820 1017 1943 2274 3793 4694 4956 820 Size: 197 926 331 1519 901 262 1553 Fragments arranged by size: 1553 1519 926 901 331 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 2935 152 Size: 2783 2906 SphI G_CATG'C Cuts at: 1132 2577 3047 1132 Size: 1445 470 3774 Fragments arranged by size: 3774 1445 470 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2608 1517 Size: 1091 4598 StyI C'CwwG_G Cuts at: 513 1065 2742 3128 513 Size: 552 1677 386 3074 Fragments arranged by size: 3074 1677 552 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2572 3716 3733 3886 4071 4516 325 Size: 1255 992 1144 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1144 992 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2419 2634 2824 Size: 274 80 33 51 1443 442 215 190 Cuts at: 2824 3935 5647 96 Size: 1111 1712 138 Fragments arranged by size: 1712 1443 1111 442 274 215 190 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2048 3014 1870 Size: 178 966 4545 Fragments arranged by size: 4545 966 178 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI