(Circular) (Six-base) MAPSORT of: pFN21AE0920.seq Check: 5589 from: 1 to: 5644 pFN21AE0920 5644 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2046 2443 2046 Size: 397 5247 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2160 2595 2987 1937 Size: 223 435 392 4594 Fragments arranged by size: 4594 435 392 223 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 2752 2769 3707 5091 5359 1632 Size: 671 449 17 938 1384 268 1917 Fragments arranged by size: 1917 1384 938 671 449 268 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2206 4543 5520 1203 Size: 1003 2337 977 1327 Fragments arranged by size: 2337 1327 1003 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 2566 4373 2566 Size: 1807 3837 AleI CACnn'nnGTG Cuts at: 1166 2258 1166 Size: 1092 4552 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2078 2276 1184 Size: 894 198 4552 Fragments arranged by size: 4552 894 198 AlwNI CAG_nnn'CTG Cuts at: 2458 3387 4959 2458 Size: 929 1572 3143 Fragments arranged by size: 3143 1572 929 ApaI G_GGCC'C Cuts at: 2129 2937 2129 Size: 808 4836 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2256 2296 2372 2616 2969 1870 Size: 73 313 40 76 244 353 4545 Fragments arranged by size: 4545 353 313 244 76 73 40 BamHI G'GATC_C Cuts at: 2640 2691 2974 3341 2640 Size: 51 283 367 4943 Fragments arranged by size: 4943 367 283 51 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2965 4420 618 Size: 325 217 627 293 885 1455 1842 Fragments arranged by size: 1842 1455 885 627 325 293 217 BanII G_rGCy'C Cuts at: 1391 2103 2129 2297 2419 2550 2937 2963 1391 Size: 712 26 168 122 131 387 26 4072 Fragments arranged by size: 4072 712 387 168 131 122 26 26 BbeI G_GCGC'C Cuts at: 2084 2084 Size: 5644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3835 3869 1903 Size: 34 50 34 1814 34 3678 Fragments arranged by size: 3678 1814 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2386 5639 2386 Size: 3253 2391 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2129 2265 2937 3705 4861 1790 Size: 339 136 672 768 1156 2573 Fragments arranged by size: 2573 1156 768 672 339 136 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2144 2242 3375 Size: 416 53 83 399 24 727 98 1133 Cuts at: 3375 4333 442 Size: 958 1753 Fragments arranged by size: 1753 1133 958 727 416 399 98 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2384 2480 2616 4304 1239 Size: 887 258 96 136 1688 2579 Fragments arranged by size: 2579 1688 887 258 136 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2534 2567 3769 4634 4932 2534 Size: 33 1202 865 298 3246 Fragments arranged by size: 3246 1202 865 298 33 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2785 2818 4307 882 Size: 617 1286 33 1489 2219 Fragments arranged by size: 2219 1489 1286 617 33 BsaAI yAC'GTr Cuts at: 493 1204 2209 3435 493 Size: 711 1005 1226 2702 Fragments arranged by size: 2702 1226 1005 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 2310 3451 3833 Size: 53 83 186 936 548 229 1141 382 Cuts at: 3833 4468 275 Size: 635 1451 Fragments arranged by size: 1451 1141 936 635 548 382 229 186 83 53 BsaWI w'CCGG_w Cuts at: 2220 4077 4475 4749 4896 5307 2220 Size: 1857 398 274 147 411 2557 Fragments arranged by size: 2557 1857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2099 2129 1178 Size: 30 165 30 696 30 4693 Fragments arranged by size: 4693 696 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 1186 Size: 1255 4389 BseYI C'CCAG_C Cuts at: 2936 4847 5343 2936 Size: 1911 496 3237 Fragments arranged by size: 3237 1911 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 1163 Size: 573 312 4759 Fragments arranged by size: 4759 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2297 2496 2550 3705 3790 4861 2297 Size: 199 54 1155 85 1071 3080 Fragments arranged by size: 3080 1155 1071 199 85 54 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 BsmBI CGTCTCn'nnnn_ Cuts at: 2182 2182 Size: 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2129 2265 2297 2419 2496 2550 Size: 399 313 26 136 32 122 77 54 Cuts at: 2550 2937 2963 3705 3790 4861 1391 Size: 387 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 313 136 122 85 77 54 32 26 26 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 2070 2443 2655 2999 844 Size: 1202 24 373 212 344 3489 Fragments arranged by size: 3489 1202 373 344 212 24 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2191 2377 4288 4475 1700 Size: 110 381 186 1911 187 2869 Fragments arranged by size: 2869 1911 381 187 186 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 2041 1925 Size: 116 5528 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 2847 4448 2847 Size: 1601 4043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2272 2077 Size: 195 5449 BstYI r'GATC_y Cuts at: 1972 2386 2640 2691 2907 2974 3341 3726 3743 Size: 414 254 51 216 67 367 385 17 Cuts at: 3743 5195 5281 5293 5639 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 414 385 367 346 254 216 86 67 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2265 2659 2867 513 Size: 552 1200 394 208 3290 Fragments arranged by size: 3290 1200 552 394 208 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2636 487 Size: 683 56 1410 3495 Fragments arranged by size: 3495 1410 683 56 BtsI GCAGTG_nn' Cuts at: 2473 3160 3954 3974 2473 Size: 687 794 20 4143 Fragments arranged by size: 4143 794 687 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2351 4651 809 Size: 507 1035 2300 1802 Fragments arranged by size: 2300 1802 1035 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 3091 3980 Size: 54 1425 50 275 18 366 895 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 895 889 366 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2396 2736 2788 3574 1353 Size: 1043 340 52 786 3423 Fragments arranged by size: 3423 1043 786 340 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2295 2548 2295 Size: 253 5391 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2303 2384 2480 2616 2752 2769 Size: 393 494 177 81 96 136 136 17 Cuts at: 2769 3707 4304 5091 5359 1239 Size: 938 597 787 268 1524 Fragments arranged by size: 1524 938 787 597 494 393 268 177 136 136 96 81 17 EcoNI CCTnn'n_nnAGG Cuts at: 2423 2891 2423 Size: 468 5176 EcoO109I rG'GnC_Cy Cuts at: 2613 2933 2613 Size: 320 5324 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2084 2579 4791 1678 Size: 406 495 2212 2531 Fragments arranged by size: 2531 2212 495 406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 4285 4317 4359 Size: 32 135 32 980 32 1701 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1701 980 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2080 2080 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 2253 4758 4942 1305 Size: 900 48 2505 184 2007 Fragments arranged by size: 2505 2007 900 184 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 2198 10 Size: 54 1425 343 366 3456 Fragments arranged by size: 3456 1425 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2270 3602 3961 4120 518 Size: 648 1092 12 1332 359 159 2042 Fragments arranged by size: 2042 1332 1092 648 359 159 12 MspA1I CmG'CkG Cuts at: 1606 2142 3060 3737 4885 5130 1606 Size: 536 918 677 1148 245 2120 Fragments arranged by size: 2120 1148 918 677 536 245 NarI GG'CG_CC Cuts at: 2081 2081 Size: 5644 NcoI C'CATG_G Cuts at: 513 1065 2265 2659 2867 513 Size: 552 1200 394 208 3290 Fragments arranged by size: 3290 1200 552 394 208 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 2686 1348 Size: 1338 4306 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PfoI T'CCnGG_A Cuts at: 2322 2361 2322 Size: 39 5605 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PmlI CAC'GTG Cuts at: 2209 2209 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2613 2613 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2125 2933 2125 Size: 808 4836 PspXI vC'TCGA_Gb Cuts at: 2256 2256 Size: 5644 PstI C_TGCA'G Cuts at: 830 2996 830 Size: 2166 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SacI G_AGCT'C Cuts at: 2297 2550 2297 Size: 253 5391 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SanDI GG'GwC_CC Cuts at: 2613 2613 Size: 5644 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SexAI A'CCwGG_T Cuts at: 2118 2118 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2992 4127 4808 4999 826 Size: 219 1947 1135 681 191 1471 Fragments arranged by size: 1947 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2082 2082 Size: 5644 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2256 2549 2582 3748 4649 4911 Size: 197 926 313 293 33 1166 901 262 Cuts at: 4911 820 Size: 1553 Fragments arranged by size: 1553 1166 926 901 313 293 262 197 33 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2265 2659 2867 3083 513 Size: 552 1200 394 208 216 3074 Fragments arranged by size: 3074 1200 552 394 216 208 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 3671 3688 3841 4026 4471 325 Size: 1255 566 1525 17 153 185 445 1498 Fragments arranged by size: 1525 1498 1255 566 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3890 5602 96 Size: 274 80 33 51 1443 1913 1712 138 Fragments arranged by size: 1913 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 2591 1316 Size: 1275 4369 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2657 2206 Size: 451 5193 XhoI C'TCGA_G Cuts at: 1943 2256 1943 Size: 313 5331 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI