(Circular) (Six-base) MAPSORT of: pFN21AE0919.seq Check: 976 from: 1 to: 5620 pFN21AE0919 5620 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2612 2626 2797 2612 Size: 14 171 5435 Fragments arranged by size: 5435 171 14 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 1937 Size: 1026 4594 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3683 5067 5335 1632 Size: 2051 1384 268 1917 Fragments arranged by size: 2051 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 2337 4519 5496 1203 Size: 1134 2182 977 1327 Fragments arranged by size: 2182 1327 1134 977 AgeI A'CCGG_T Cuts at: 2703 4451 2703 Size: 1748 3872 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2187 1184 Size: 1003 4617 AlwNI CAG_nnn'CTG Cuts at: 3363 4935 3363 Size: 1572 4048 ApaI G_GGCC'C Cuts at: 2078 2223 2078 Size: 145 5475 ApaLI G'TGCA_C Cuts at: 2360 3677 4833 2360 Size: 1317 1156 3147 Fragments arranged by size: 3147 1317 1156 ApoI r'AATT_y Cuts at: 1493 2435 2929 3155 3307 5416 1493 Size: 942 494 226 152 2109 1697 Fragments arranged by size: 2109 1697 942 494 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2585 2945 1870 Size: 73 127 515 360 4545 Fragments arranged by size: 4545 515 360 127 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2248 2941 4396 618 Size: 325 217 627 461 693 1455 1842 Fragments arranged by size: 1842 1455 693 627 461 325 217 BanII G_rGCy'C Cuts at: 1391 2078 2223 2585 2890 2939 1391 Size: 687 145 362 305 49 4072 Fragments arranged by size: 4072 687 362 305 145 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4649 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2047 2081 3811 3845 1903 Size: 34 110 34 1730 34 3678 Fragments arranged by size: 3678 1730 110 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 2595 2727 1548 Size: 1047 132 4441 Fragments arranged by size: 4441 1047 132 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 2889 3037 2889 Size: 148 5472 Bme1580I G_kGCm'C Cuts at: 1790 2078 2223 2364 3681 4837 1790 Size: 288 145 141 1317 1156 2573 Fragments arranged by size: 2573 1317 1156 288 145 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2551 2807 4280 1239 Size: 1097 215 256 1473 2579 Fragments arranged by size: 2579 1473 1097 256 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2444 2732 3745 4610 4908 2444 Size: 288 1013 865 298 3156 Fragments arranged by size: 3156 1013 865 298 288 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2569 2828 4283 882 Size: 617 1070 259 1455 2219 Fragments arranged by size: 2219 1455 1070 617 259 BsaAI yAC'GTr Cuts at: 493 1204 2340 3411 493 Size: 711 1136 1071 2702 Fragments arranged by size: 2702 1136 1071 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2703 4053 4451 4725 4872 5283 2703 Size: 1350 398 274 147 411 3040 Fragments arranged by size: 3040 1350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5395 Fragments arranged by size: 5395 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5620 BseYI C'CCAG_C Cuts at: 2086 4823 5319 2086 Size: 2737 496 2387 Fragments arranged by size: 2737 2387 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2007 1163 Size: 573 271 4776 Fragments arranged by size: 4776 573 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2083 3070 3831 4859 1540 Size: 275 186 82 987 761 1028 2301 Fragments arranged by size: 2301 1028 987 761 275 186 82 BsiHKAI G_wGCw'C Cuts at: 2364 2883 3681 3766 4837 2364 Size: 519 798 85 1071 3147 Fragments arranged by size: 3147 1071 798 519 85 BsmI GAATG_Cn' Cuts at: 3131 3224 3131 Size: 93 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2223 2364 2585 2883 2890 2939 Size: 399 288 145 141 221 298 7 49 Cuts at: 2939 3681 3766 4837 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 298 288 221 145 141 85 49 7 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2612 2626 2797 2975 844 Size: 1768 14 171 178 3489 Fragments arranged by size: 3489 1768 178 171 14 BsrBI CCG'CTC Cuts at: 1394 1823 2713 3507 1394 Size: 429 890 794 3507 Fragments arranged by size: 3507 890 794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2847 4115 4289 65 Size: 1183 1599 1268 174 1396 Fragments arranged by size: 1599 1396 1268 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2703 4264 4451 1700 Size: 110 893 1561 187 2869 Fragments arranged by size: 2869 1561 893 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 2472 4424 2472 Size: 1952 3668 BstEII G'GTnAC_C Cuts at: 1184 2812 1184 Size: 1628 3992 BstYI r'GATC_y Cuts at: 1972 2464 2950 3317 3702 3719 5171 5257 5269 Size: 492 486 367 385 17 1452 86 12 Cuts at: 5269 5615 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 492 486 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 2255 2409 513 Size: 552 939 36 215 154 3724 Fragments arranged by size: 3724 939 552 215 154 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2214 487 Size: 683 56 988 3893 Fragments arranged by size: 3893 988 683 56 BtsI GCAGTG_nn' Cuts at: 3136 3930 3950 3136 Size: 794 20 4806 Fragments arranged by size: 4806 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2274 2368 Size: 54 1425 50 275 18 250 194 94 Cuts at: 2368 3067 3956 5585 8 Size: 699 889 1629 43 Fragments arranged by size: 1629 1425 889 699 275 250 194 94 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2080 3067 1537 Size: 275 268 987 4090 Fragments arranged by size: 4090 987 275 268 EarI CTCTTCn'nnn_ Cuts at: 1353 2474 3550 1353 Size: 1121 1076 3423 Fragments arranged by size: 3423 1121 1076 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2026 2408 4207 4581 4727 387 Size: 1064 138 437 382 1799 374 146 1280 Fragments arranged by size: 1799 1280 1064 437 382 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2551 2807 3683 4280 5067 5335 Size: 393 704 215 256 876 597 787 268 Cuts at: 5335 1239 Size: 1524 Fragments arranged by size: 1524 876 787 704 597 393 268 256 215 EcoNI CCTnn'n_nnAGG Cuts at: 2196 2196 Size: 5620 EcoO109I rG'GnC_Cy Cuts at: 2074 2075 2219 2329 2480 2074 Size: 1 144 110 151 5214 Fragments arranged by size: 5214 151 144 110 1 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 5620 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4261 4293 4335 4367 1373 Size: 32 135 32 2689 32 42 32 2626 Fragments arranged by size: 2689 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 4734 4918 1305 Size: 972 2457 184 2007 Fragments arranged by size: 2457 2007 972 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 2370 10 Size: 54 1425 343 444 94 3260 Fragments arranged by size: 3260 1425 444 343 94 54 MslI CAynn'nnrTG Cuts at: 518 1166 2254 3578 3937 4096 518 Size: 648 1088 1324 359 159 2042 Fragments arranged by size: 2042 1324 1088 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2188 3036 3713 4861 5106 1606 Size: 582 848 677 1148 245 2120 Fragments arranged by size: 2120 1148 848 677 582 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2255 513 Size: 552 939 251 3878 Fragments arranged by size: 3878 939 552 251 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2978 4523 1132 Size: 97 1749 1545 2229 Fragments arranged by size: 2229 1749 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 2289 1108 Size: 1181 4439 PfoI T'CCnGG_A Cuts at: 2313 2439 2313 Size: 126 5494 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2329 2480 2329 Size: 151 5469 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PspOMI G'GGCC_C Cuts at: 2074 2219 2074 Size: 145 5475 PstI C_TGCA'G Cuts at: 830 2218 2808 2972 830 Size: 1388 590 164 3478 Fragments arranged by size: 3478 1388 590 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SanDI GG'GwC_CC Cuts at: 2480 2480 Size: 5620 SbfI CC_TGCA'GG Cuts at: 2218 2808 2972 2218 Size: 590 164 4866 Fragments arranged by size: 4866 590 164 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2214 2386 2804 2902 2968 4103 4784 Size: 219 1169 172 418 98 66 1135 681 Cuts at: 4784 4975 826 Size: 191 1471 Fragments arranged by size: 1471 1169 1135 681 418 219 191 172 98 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2072 2947 1872 Size: 200 875 4545 Fragments arranged by size: 4545 875 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 2459 2747 3724 4625 4887 820 Size: 197 926 516 288 977 901 262 1553 Fragments arranged by size: 1553 977 926 901 516 288 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5620 StyI C'CwwG_G Cuts at: 513 1065 2004 2255 3059 513 Size: 552 939 251 804 3074 Fragments arranged by size: 3074 939 804 552 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3647 3664 3817 4002 4447 325 Size: 1255 2067 17 153 185 445 1498 Fragments arranged by size: 2067 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2721 2766 3866 Size: 274 80 33 51 1443 744 45 1100 Cuts at: 3866 5578 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1100 744 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2806 2806 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2070 2945 1870 Size: 200 875 4545 Fragments arranged by size: 4545 875 200 XmnI GAAnn'nnTTC Cuts at: 2153 3749 2153 Size: 1596 4024 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI