Restriction map for
pFN21AE0919
(Linear) (Six-base) MAPSORT of: KIEE0919.seq Check: 1005 from: 1 to: 915
KIEE0919.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 607 621 792 915
Size: 607 14 171 123
Fragments arranged by size:
607 171 123 14
AflIII A'CryG_T
Cuts at: 0 332 915
Size: 332 583
AgeI A'CCGG_T
Cuts at: 0 698 915
Size: 698 217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 182 915
Size: 182 733
ApaI G_GGCC'C
Cuts at: 0 73 218 915
Size: 73 145 697
Fragments arranged by size:
697 145 73
ApaLI G'TGCA_C
Cuts at: 0 355 915
Size: 355 560
ApoI r'AATT_y
Cuts at: 0 430 915
Size: 430 485
AvaI C'yCGr_G
Cuts at: 0 65 580 915
Size: 65 515 335
Fragments arranged by size:
515 335 65
BanI G'GyrC_C
Cuts at: 0 243 915
Size: 243 672
BanII G_rGCy'C
Cuts at: 0 73 218 580 885 915
Size: 73 145 362 305 30
Fragments arranged by size:
362 305 145 73 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 76 915
Size: 42 34 839
Fragments arranged by size:
839 42 34
BclI T'GATC_A
Cuts at: 0 590 722 915
Size: 590 132 193
Fragments arranged by size:
590 193 132
BlpI GC'TnA_GC
Cuts at: 0 884 915
Size: 884 31
Bme1580I G_kGCm'C
Cuts at: 0 73 218 359 915
Size: 73 145 141 556
Fragments arranged by size:
556 145 141 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 546 802 915
Size: 331 215 256 113
Fragments arranged by size:
331 256 215 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 727 915
Size: 439 288 188
Fragments arranged by size:
439 288 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 564 823 915
Size: 564 259 92
Fragments arranged by size:
564 259 92
BsaAI yAC'GTr
Cuts at: 0 335 915
Size: 335 580
BsaWI w'CCGG_w
Cuts at: 0 698 915
Size: 698 217
BseYI C'CCAG_C
Cuts at: 0 81 915
Size: 81 834
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 915
Size: 2 913
BsiEI CG_ry'CG
Cuts at: 0 78 915
Size: 78 837
BsiHKAI G_wGCw'C
Cuts at: 0 359 878 915
Size: 359 519 37
Fragments arranged by size:
519 359 37
Bsp1286I G_dGCh'C
Cuts at: 0 73 218 359 580 878 885 915
Size: 73 145 141 221 298 7 30
Fragments arranged by size:
298 221 145 141 73 30 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 607 621 792 915
Size: 607 14 171 123
Fragments arranged by size:
607 171 123 14
BsrBI CCG'CTC
Cuts at: 0 708 915
Size: 708 207
BsrDI GCAATG_nn'
Cuts at: 0 842 915
Size: 842 73
BsrFI r'CCGG_y
Cuts at: 0 698 915
Size: 698 217
BstBI TT'CG_AA
Cuts at: 0 467 915
Size: 467 448
BstEII G'GTnAC_C
Cuts at: 0 807 915
Size: 807 108
BstYI r'GATC_y
Cuts at: 0 459 915
Size: 459 456
BtgI C'CryG_G
Cuts at: 0 35 250 404 915
Size: 35 215 154 511
Fragments arranged by size:
511 215 154 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 209 915
Size: 209 706
EaeI y'GGCC_r
Cuts at: 0 75 269 363 915
Size: 75 194 94 552
Fragments arranged by size:
552 194 94 75
EagI C'GGCC_G
Cuts at: 0 75 915
Size: 75 840
EarI CTCTTCn'nnn_
Cuts at: 0 469 915
Size: 469 446
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 21 403 915
Size: 21 382 512
Fragments arranged by size:
512 382 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 546 802 915
Size: 331 215 256 113
Fragments arranged by size:
331 256 215 113
EcoNI CCTnn'n_nnAGG
Cuts at: 0 191 915
Size: 191 724
EcoO109I rG'GnC_Cy
Cuts at: 0 69 70 214 324 475 915
Size: 69 1 144 110 151 440
Fragments arranged by size:
440 151 144 110 69 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 480 915
Size: 480 435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 915
Size: 272 643
MscI TGG'CCA
Cuts at: 0 271 365 915
Size: 271 94 550
Fragments arranged by size:
550 271 94
MslI CAynn'nnrTG
Cuts at: 0 249 915
Size: 249 666
MspA1I CmG'CkG
Cuts at: 0 183 915
Size: 183 732
NcoI C'CATG_G
Cuts at: 0 250 915
Size: 250 665
PflMI CCAn_nnn'nTGG
Cuts at: 0 284 915
Size: 284 631
PfoI T'CCnGG_A
Cuts at: 0 308 434 915
Size: 308 126 481
Fragments arranged by size:
481 308 126
PpuMI rG'GwC_Cy
Cuts at: 0 324 475 915
Size: 324 151 440
Fragments arranged by size:
440 324 151
PspOMI G'GGCC_C
Cuts at: 0 69 214 915
Size: 69 145 701
Fragments arranged by size:
701 145 69
PstI C_TGCA'G
Cuts at: 0 213 803 915
Size: 213 590 112
Fragments arranged by size:
590 213 112
SanDI GG'GwC_CC
Cuts at: 0 475 915
Size: 475 440
SbfI CC_TGCA'GG
Cuts at: 0 213 803 915
Size: 213 590 112
Fragments arranged by size:
590 213 112
SfcI C'TryA_G
Cuts at: 0 209 381 799 897 915
Size: 209 172 418 98 18
Fragments arranged by size:
418 209 172 98 18
SmaI CCC'GGG
Cuts at: 0 67 915
Size: 67 848
SmlI C'TyrA_G
Cuts at: 0 454 742 915
Size: 454 288 173
Fragments arranged by size:
454 288 173
StyI C'CwwG_G
Cuts at: 0 250 915
Size: 250 665
TatI w'GTAC_w
Cuts at: 0 716 761 915
Size: 716 45 154
Fragments arranged by size:
716 154 45
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 801 915
Size: 801 114
XmaI C'CCGG_G
Cuts at: 0 65 915
Size: 65 850
XmnI GAAnn'nnTTC
Cuts at: 0 148 915
Size: 148 767
Enzymes that do cut:
AarI AflIII AgeI AlfI ApaI ApaLI ApoI AvaI
BanI BanII BcgI BclI BlpI Bme1580I BpmI BpuEI
BsaI BsaAI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstBI BstEII BstYI BtgI
BtgZI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI MmeI MscI MslI MspA1I NcoI PflMI PfoI
PpuMI PspOMI PstI SanDI SbfI SfcI SmaI SmlI
StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BciVI BfrBI BglI BglII
BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaHI BsaXI
BseRI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI ZraI