Restriction map for
pFN21AE0913
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AvrII BamHI BanI
BbeI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MroI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 214 GAATTT [GA]^AATT[CT]
AfaI 328 GTAC GT^AC
AflIII 87 ACATGT A^C[GA][CT]GT
AhdI 672 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 214 GAATTT [GA]^AATT[CT]
AspEI 672 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 701 GGACC G^G[AT]CC
AviII 725 TGCGCA TGC^GCA
BanII 376 GAGCCC G[GA]GC[CT]^C
BbrPI 432 CACGTG CAC^GTG
BbvI 739 GCAGC GCAGC(8/12)
BclI 92 TGATCA T^GATCA
BfaI 25 CTAG C^TAG
BpmI 332 CTCCAG CTGGAG(16/14)
BsaAI 432 CACGTG [CT]AC^GT[GA]
BsmBI 193 GAGACG CGTCTC(1/5)
BspHI 290 TCATGA T^CATGA
BspLU11I 87 ACATGT A^CATGT
BsrDI 734 GCAATG GCAATG(2/0)
BstBI 440 TTCGAA TT^CGAA
Cac8I 229 GCCAGC GC[ACGT]^[ACGT]GC
DraII 753 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 672 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 593 CTCTTC CTCTTC(1/4)
EcoNI 19 CCTGTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 753 GGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 92 TGATCA T^GATCA
FspI 725 TGCGCA TGC^GCA
Ksp632I 593 CTCTTC CTCTTC(1/4)
MaeI 25 CTAG C^TAG
NspI 87 ACATGT [GA]CATG^[CT]
NspV 440 TTCGAA TT^CGAA
PleI 14 GAGTC GAGTC(4/5)
PmaCI 432 CACGTG CAC^GTG
PmlI 432 CACGTG CAC^GTG
PstI 561 CTGCAG CTGCA^G
RcaI 290 TCATGA T^CATGA
RsaI 328 GTAC GT^AC
SfuI 440 TTCGAA TT^CGAA
TseI 739 GCAGC G^C[AT]GC
Tsp509I 215 AATT ^AATT
XcmI 257 CCAACATAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 510 CCGC CCGC(-3/-1)
204 GCGG
Aor51HI 55 AGCGCT AGC^GCT
299 AGCGCT
AspHI 346 GTGCTC G[AT]GC[AT]^C
571 GAGCAC
BalI 2 TGGCCA TGG^CCA
254 TGGCCA
BbsI 496 GAAGAC GAAGAC(2/6)
481 GTCTTC
BpuAI 496 GAAGAC GAAGAC(2/6)
481 GTCTTC
BsiHKAI 346 GTGCTC G[AT]GC[AT]^C
571 GAGCAC
BsmFI 700 GGGAC GGGAC(10/14)
16 GTCCC
BstF5I 96 CATCC GGATG(2/0)
585 GGATG
DpnII 93 GATC ^GATC
318 GATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
254 TGGCCA
Eco47III 55 AGCGCT AGC^GCT
299 AGCGCT
Fnu4HI 204 GCGGC GC^[ACGT]GC
739 GCAGC
FokI 96 CATCC GGATG(9/13)
585 GGATG
HaeII 55 AGCGCT [GA]GCGC^[CT]
299 AGCGCT
ItaI 204 GCGGC GC^[ACGT]GC
739 GCAGC
MboI 93 GATC ^GATC
318 GATC
MluNI 2 TGGCCA TGG^CCA
254 TGGCCA
MscI 2 TGGCCA TGG^CCA
254 TGGCCA
MwoI 241 GCTCAAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
256 GCCAACATAGC
NdeII 93 GATC ^GATC
318 GATC
NlaIV 700 GGGACC GG[ACGT]^[ACGT]CC
753 GGGGCC
Sau3AI 93 GATC ^GATC
318 GATC
SfaNI 616 GATGC GCATC(5/9)
689 GCATC
SfcI 561 CTGCAG C^T[GA][CT]AG
641 CTACAG
SmlI 242 CTCAAG C^T[CT][GA]AG
598 CTCAAG
TaqI 401 TCGA T^CGA
441 TCGA
TfiI 64 GAATC G^A[AT]TC
117 GAATC
Tsp45I 430 GTCAC ^GT[GC]AC
457 GTCAC
TthHB8I 401 TCGA T^CGA
441 TCGA
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
CfoI Cfr13I DdeI EcoRII HaeIII
HhaI HinP1I HinfI HphI MaeII
MaeIII MboII MnlI MslI MvaI
NlaIII Sau96I ScrFI TspRI