Restriction map for
pFN21AE0913
(Linear) (Six-base) MAPSORT of: KIEE0913.seq Check: 2026 from: 1 to: 762
KIEE0913.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AfeI AGC'GCT
Cuts at: 0 57 301 762
Size: 57 244 461
Fragments arranged by size:
461 244 57
AflIII A'CryG_T
Cuts at: 0 87 762
Size: 87 675
AhdI GACnn_n'nnGTC
Cuts at: 0 677 762
Size: 677 85
AleI CACnn'nnGTG
Cuts at: 0 466 762
Size: 466 296
ApoI r'AATT_y
Cuts at: 0 214 762
Size: 214 548
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 311 344 762
Size: 311 33 418
Fragments arranged by size:
418 311 33
BanII G_rGCy'C
Cuts at: 0 380 762
Size: 380 382
BbsI GAAGACnn'nnnn_
Cuts at: 0 474 503 762
Size: 474 29 259
Fragments arranged by size:
474 259 29
BclI T'GATC_A
Cuts at: 0 92 762
Size: 92 670
Bme1580I G_kGCm'C
Cuts at: 0 663 762
Size: 663 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 585 615 762
Size: 585 30 147
Fragments arranged by size:
585 147 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 762
Size: 317 445
Bpu10I CC'TnA_GC
Cuts at: 0 59 762
Size: 59 703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 583 762
Size: 227 356 179
Fragments arranged by size:
356 227 179
BsaAI yAC'GTr
Cuts at: 0 434 762
Size: 434 328
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 762
Size: 5 30 727
Fragments arranged by size:
727 30 5
BsiHKAI G_wGCw'C
Cuts at: 0 350 575 762
Size: 350 225 187
Fragments arranged by size:
350 225 187
BsmBI CGTCTCn'nnnn_
Cuts at: 0 187 762
Size: 187 575
Bsp1286I G_dGCh'C
Cuts at: 0 350 380 575 663 762
Size: 350 30 195 88 99
Fragments arranged by size:
350 195 99 88 30
BspHI T'CATG_A
Cuts at: 0 290 762
Size: 290 472
BsrDI GCAATG_nn'
Cuts at: 0 741 762
Size: 741 21
BstBI TT'CG_AA
Cuts at: 0 441 762
Size: 441 321
EaeI y'GGCC_r
Cuts at: 0 2 254 762
Size: 2 252 508
Fragments arranged by size:
508 252 2
EarI CTCTTCn'nnn_
Cuts at: 0 599 762
Size: 599 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 762
Size: 317 445
EcoNI CCTnn'n_nnAGG
Cuts at: 0 23 762
Size: 23 739
EcoO109I rG'GnC_Cy
Cuts at: 0 754 762
Size: 754 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 520 762
Size: 520 242
FspI TGC'GCA
Cuts at: 0 727 762
Size: 727 35
HaeII r_GCGC'y
Cuts at: 0 59 303 762
Size: 59 244 459
Fragments arranged by size:
459 244 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 42 52 74 663 695 762
Size: 20 22 10 22 589 32 67
Fragments arranged by size:
589 67 32 22 22 20 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 762
Size: 329 433
MscI TGG'CCA
Cuts at: 0 4 256 762
Size: 4 252 506
Fragments arranged by size:
506 252 4
MslI CAynn'nnrTG
Cuts at: 0 43 265 466 762
Size: 43 222 201 296
Fragments arranged by size:
296 222 201 43
NspI r_CATG'y
Cuts at: 0 91 762
Size: 91 671
PasI CC'CwG_GG
Cuts at: 0 491 663 762
Size: 491 172 99
Fragments arranged by size:
491 172 99
PciI A'CATG_T
Cuts at: 0 87 762
Size: 87 675
PmlI CAC'GTG
Cuts at: 0 434 762
Size: 434 328
PstI C_TGCA'G
Cuts at: 0 565 762
Size: 565 197
SfcI C'TryA_G
Cuts at: 0 561 641 762
Size: 561 80 121
Fragments arranged by size:
561 121 80
SmlI C'TyrA_G
Cuts at: 0 242 598 762
Size: 242 356 164
Fragments arranged by size:
356 242 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5 37 762
Size: 5 32 725
Fragments arranged by size:
725 32 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 762
Size: 264 498
Enzymes that do cut:
AfeI AflIII AhdI AleI ApoI BaeI BanII BbsI
BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaXI
BsiHKAI BsmBI Bsp1286I BspHI BsrDI BstBI EaeI EarI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MmeI
MscI MslI NspI PasI PciI PmlI PstI SfcI
SmlI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiWI
BsmI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI
ZraI