Restriction map for
pFN21AE0911
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BalI BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BsrI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 199 GGCGTC G[GA]^CG[CT]C
ApaI 161 GGGCCC GGGCC^C
Asp700I 513 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 121 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 488 GGATCC G^GATCC
BbsI 712 GAAGAC GAAGAC(2/6)
BfaI 686 CTAG C^TAG
BglI 320 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 712 GAAGAC GAAGAC(2/6)
BsaHI 199 GGCGTC G[GA]^CG[CT]C
BseRI 253 GAGGAG GAGGAG(10/8)
BsmAI 202 GTCTC GTCTC(1/5)
BsmBI 201 CGTCTC CGTCTC(1/5)
BspHI 17 TCATGA T^CATGA
BsrDI 358 GCAATG GCAATG(2/0)
BssHII 192 GCGCGC G^CGCGC
BssSI 70 CACGAG CACGAG(-5/-1)
BstAPI 434 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 468 GGATG GGATG(2/0)
BstYI 488 GGATCC [GA]^GATC[CT]
DdeI 353 CTAAG C^T[ACGT]AG
DrdI 295 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 174 CGGCCA [CT]^GGCC[GA]
EcoNI 102 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 468 GGATG GGATG(9/13)
Hin1I 199 GGCGTC G[GA]^CG[CT]C
MaeI 686 CTAG C^TAG
MflI 488 GGATCC [GA]^GATC[CT]
MseI 682 TTAA T^TAA
PflFI 121 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 551 GACTC GAGTC(4/5)
RcaI 17 TCATGA T^CATGA
SgrAI 195 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 293 TCGA T^CGA
TfiI 697 GAATC G^A[AT]TC
Tru9I 682 TTAA T^TAA
Tth111I 121 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 293 TCGA T^CGA
XhoII 488 GGATCC [GA]^GATC[CT]
XmnI 513 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 670 AAATTC [GA]^AATT[CT]
678 GAATTT
AfaI 51 GTAC GT^AC
633 GTAC
ApoI 670 AAATTC [GA]^AATT[CT]
678 GAATTT
AvaII 27 GGACC G^G[AT]CC
303 GGTCC
BanII 161 GGGCCC G[GA]GC[CT]^C
535 GAGCCC
BbrPI 234 CACGTG CAC^GTG
403 CACGTG
BcnI 158 CCGGG CC^[GC]GG
376 CCGGG
BsaAI 234 CACGTG [CT]AC^GT[GA]
403 CACGTG
BspMI 215 ACCTGC ACCTGC(4/8)
439 ACCTGC
BsrFI 196 GCCGGC [GA]^CCGG[CT]
320 GCCGGC
Cfr10I 196 GCCGGC [GA]^CCGG[CT]
320 GCCGGC
DraII 160 GGGGCCC [GA]G^G[ACGT]CC[CT]
161 GGGCCCT
DraIII 70 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
586 CACGAAGTG
EcoO109I 160 GGGGCCC [GA]G^G[ACGT]CC[CT]
161 GGGCCCT
HinfI 551 GACTC G^A[ACGT]TC
697 GAATC
HphI 421 GGTGA GGTGA(8/7)
437 TCACC
MspA1I 145 CAGCTG C[AC]G^C[GT]G
178 CAGCTG
NaeI 196 GCCGGC GCC^GGC
320 GCCGGC
NciI 158 CCGGG CC^[GC]GG
376 CCGGG
NgoMIV 196 GCCGGC G^CCGGC
320 GCCGGC
NlaIII 18 CATG CATG^
481 CATG
PmaCI 234 CACGTG CAC^GTG
403 CACGTG
PmlI 234 CACGTG CAC^GTG
403 CACGTG
PvuII 145 CAGCTG CAG^CTG
178 CAGCTG
RsaI 51 GTAC GT^AC
633 GTAC
TspRI 151 CACTG CA[GC]TG[ACGT][ACGT]^
708 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DpnII
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
MaeII MboI MboII MnlI MspI
MvaI MvnI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp509I