Restriction map for
pFN21AE0910
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BseRI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI Eam1105I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AciI 307 CCGC CCGC(-3/-1)
AluI 568 AGCT AG^CT
AocI 187 CCTGAGG CC^T[ACGT]AGG
Aor51HI 426 AGCGCT AGC^GCT
AvaII 623 GGTCC G^G[AT]CC
BalI 508 TGGCCA TGG^CCA
BamHI 58 GGATCC G^GATCC
BanII 655 GAGCCC G[GA]GC[CT]^C
BbsI 471 GAAGAC GAAGAC(2/6)
BmyI 655 GAGCCC G[AGT]GC[ACT]^C
BpuAI 471 GAAGAC GAAGAC(2/6)
BsaI 236 GGTCTC GGTCTC(1/5)
BsaWI 403 TCCGGT [AT]^CCGG[AT]
BsiEI 455 CGGCCG CG[GA][CT]^CG
BsmAI 237 GTCTC GTCTC(1/5)
BsmFI 624 GTCCC GGGAC(10/14)
Bsp1286I 655 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 464 TGTACA T^GTACA
BspMI 4 GCAGGT ACCTGC(4/8)
BsrGI 464 TGTACA T^GTACA
BstYI 58 GGATCC [GA]^GATC[CT]
Bsu36I 187 CCTGAGG CC^T[ACGT]AGG
CfoI 427 GCGC GCG^C
DraI 544 TTTAAA TTT^AAA
DsaI 443 CCACGG C^C[GA][CT]GG
EagI 455 CGGCCG C^GGCCG
EarI 652 GAAGAG CTCTTC(1/4)
EclXI 455 CGGCCG C^GGCCG
Eco47III 426 AGCGCT AGC^GCT
Eco52I 455 CGGCCG C^GGCCG
Eco81I 187 CCTGAGG CC^T[ACGT]AGG
EcoRV 163 GATATC GAT^ATC
FauI 306 CCCGC CCCGC(4/6)
HaeII 426 AGCGCT [GA]GCGC^[CT]
HapII 404 CCGG C^CGG
HhaI 427 GCGC GCG^C
HinP1I 427 GCGC G^CGC
HpaII 404 CCGG C^CGG
Ksp632I 652 GAAGAG CTCTTC(1/4)
MflI 58 GGATCC [GA]^GATC[CT]
MluNI 508 TGGCCA TGG^CCA
MscI 508 TGGCCA TGG^CCA
MseI 545 TTAA T^TAA
MslI 532 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 307 CCGCTG C[AC]G^C[GT]G
MspI 404 CCGG C^CGG
NheI 244 GCTAGC G^CTAGC
PleI 479 GAGTC GAGTC(4/5)
PshAI 474 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 634 CTGCAG CTGCA^G
SapI 652 GAAGAGC GCTCTTC(1/4)
ScaI 15 AGTACT AGT^ACT
SfaNI 586 GCATC GCATC(5/9)
SspBI 464 TGTACA T^GTACA
TaqI 495 TCGA T^CGA
Tru9I 545 TTAA T^TAA
Tsp45I 109 GTCAC ^GT[GC]AC
TthHB8I 495 TCGA T^CGA
XhoII 58 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 59 GATCC GGATC(4/5)
58 GGATC
AlwNI 113 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
628 CAGAGGCTG
BpmI 412 CTCCAG CTGGAG(16/14)
514 CTCCAG
BsgI 102 CTGCAC GTGCAG(16/14)
666 CTGCAC
Cfr13I 149 GGGCC G^G[ACGT]CC
623 GGTCC
DpnII 59 GATC ^GATC
564 GATC
EaeI 455 CGGCCG [CT]^GGCC[GA]
508 TGGCCA
MaeIII 109 GTCAC ^GT[ACGT]AC
439 GTAAC
MboI 59 GATC ^GATC
564 GATC
NdeII 59 GATC ^GATC
564 GATC
NlaIII 132 CATG CATG^
520 CATG
Sau3AI 59 GATC ^GATC
564 GATC
Sau96I 149 GGGCC G^G[ACGT]CC
623 GGTCC
SfcI 77 CTGTAG C^T[GA][CT]AG
634 CTGCAG
Tsp509I 199 AATT ^AATT
582 AATT
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI BbvI BfaI BsaJI BsrI
BssKI BstF5I BstNI Cac8I DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MboII MnlI MvaI
MwoI NlaIV RsaI ScrFI TfiI
TseI TspRI