################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AclI AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspI AviII AvrII BanI BbeI BbrPI BbsI BcgI BciVI BclI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsiWI BspDI BspLU11I BsrBI BsrDI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraIII Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII KasI KpnI MfeI MluI MunI NarI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccII 30 CGCG CG^CG AccIII 1331 TCCGGA T^CCGGA AcyI 2319 GGCGTC G[GA]^CG[CT]C AflII 252 CTTAAG C^TTAAG AhdI 1425 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1425 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 476 CCCGGG C^[CT]CG[GA]G BbvCI 1231 CCTCAGC CCTCAGC(-5/-2) BfrI 252 CTTAAG C^TTAAG BglII 430 AGATCT A^GATCT BsaHI 2319 GGCGTC G[GA]^CG[CT]C BsaWI 1331 TCCGGA [AT]^CCGG[AT] BseAI 1331 TCCGGA T^CCGGA BsgI 1313 GTGCAG GTGCAG(16/14) BsiEI 38 CGGCCG CG[GA][CT]^CG BsmBI 1481 GAGACG CGTCTC(1/5) BsmI 909 GAATGC GAATGC(1/-1) BsoBI 476 CCCGGG C^[CT]CG[GA]G Bsp1407I 1200 TGTACA T^GTACA BspEI 1331 TCCGGA T^CCGGA BspHI 1080 TCATGA T^CATGA BspMI 930 GCAGGT ACCTGC(4/8) BsrFI 40 GCCGGC [GA]^CCGG[CT] BsrGI 1200 TGTACA T^GTACA BssSI 879 CACGAG CACGAG(-5/-1) BstAPI 205 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 862 GGTAACC G^GT[ACGT]ACC BstPI 862 GGTAACC G^GT[ACGT]ACC BstUI 30 CGCG CG^CG CfoI 1656 GCGC GCG^C Cfr10I 40 GCCGGC [GA]^CCGG[CT] DrdI 1215 GACAGTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 29 CCGCGG C^C[GA][CT]GG EagI 38 CGGCCG C^GGCCG Eam1105I 1425 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 38 CGGCCG C^GGCCG Eco52I 38 CGGCCG C^GGCCG EcoO65I 862 GGTAACC G^GT[ACGT]ACC HaeII 1655 GGCGCT [GA]GCGC^[CT] HhaI 1656 GCGC GCG^C Hin1I 2319 GGCGTC G[GA]^CG[CT]C HinP1I 1656 GCGC G^CGC HpaI 1726 GTTAAC GTT^AAC KspI 29 CCGCGG CCGC^GG MroI 1331 TCCGGA T^CCGGA MvnI 30 CGCG CG^CG NaeI 40 GCCGGC GCC^GGC NdeI 599 CATATG CA^TATG NgoMIV 40 GCCGGC G^CCGGC PflMI 867 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1080 TCATGA T^CATGA SacI 1176 GAGCTC GAGCT^C SacII 29 CCGCGG CCGC^GG SapI 1768 GAAGAGC GCTCTTC(1/4) ScaI 2332 AGTACT AGT^ACT SmaI 476 CCCGGG CCC^GGG SpeI 955 ACTAGT A^CTAGT SspBI 1200 TGTACA T^GTACA StuI 1942 AGGCCT AGG^CCT Tsp45I 916 GTCAC ^GT[GC]AC Van91I 867 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 667 CCATATTTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 476 CCCGGG C^CCGGG XmaI 476 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AseI 268 ATTAAT AT^TAAT 2020 ATTAAT AsnI 268 ATTAAT AT^TAAT 2020 ATTAAT AspHI 1176 GAGCTC G[AT]GC[AT]^C 1803 GAGCAC BalI 573 TGGCCA TGG^CCA 875 TGGCCA BamHI 546 GGATCC G^GATCC 1627 GGATCC BcnI 476 CCCGG CC^[GC]GG 477 CCGGG BseRI 2399 CTCCTC GAGGAG(10/8) 1420 GAGGAG BsiHKAI 1176 GAGCTC G[AT]GC[AT]^C 1803 GAGCAC Cac8I 40 GCCGGC GC[ACGT]^[ACGT]GC 571 GCTGGC DraI 222 TTTAAA TTT^AAA 699 TTTAAA DraII 1227 AGGTCCT [GA]G^G[ACGT]CC[CT] 1464 AGGACCT EarI 850 CTCTTC CTCTTC(1/4) 1768 GAAGAG EcoO109I 1227 AGGTCCT [GA]G^G[ACGT]CC[CT] 1464 AGGACCT EcoT14I 1651 CCAAGG C^C[AT][AT]GG 2292 CCTTGG FauI 28 CCCGC CCCGC(4/6) 4 GCGGG HincII 1582 GTTGAC GT[CT]^[GA]AC 1726 GTTAAC HindII 1582 GTTGAC GT[CT]^[GA]AC 1726 GTTAAC Ksp632I 850 CTCTTC CTCTTC(1/4) 1768 GAAGAG MaeII 68 ACGT A^CGT 1336 ACGT MluNI 573 TGGCCA TGG^CCA 875 TGGCCA MscI 573 TGGCCA TGG^CCA 875 TGGCCA MspA1I 29 CCGCGG C[AC]G^C[GT]G 45 CAGCGG NciI 476 CCCGG CC^[GC]GG 477 CCGGG PpuMI 1227 AGGTCCT [GA]G^G[AT]CC[CT] 1464 AGGACCT PshBI 268 ATTAAT AT^TAAT 2020 ATTAAT SexAI 393 ACCAGGT A^CC[AT]GGT 2358 ACCTGGT SfcI 461 CTACAG C^T[GA][CT]AG 839 CTGTAG StyI 1651 CCAAGG C^C[AT][AT]GG 2292 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanII BbvI BfaI BmyI BpmI BsaBI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII