(Circular) (Six-base) MAPSORT of: pFN21AE4231.seq Check: 4700 from: 1 to: 7150 pFN21AE4231 7150 bp With 182 enzymes: SgfI * March 28, 2012 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4960 278 Size: 53 83 186 936 3424 2468 Fragments arranged by size: 3424 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4493 1937 Size: 2556 4594 Acc65I G'GTAC_C Cuts at: 4471 4471 Size: 7150 AclI AA'CG_TT Cuts at: 1563 5277 5650 1563 Size: 3714 373 3063 Fragments arranged by size: 3714 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2628 3878 3884 4110 4221 4413 5213 6597 Size: 996 1250 6 226 111 192 800 1384 Cuts at: 6597 6865 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1250 996 800 268 226 192 111 6 AflII C'TTAA_G Cuts at: 820 1017 2257 820 Size: 197 1240 5713 Fragments arranged by size: 5713 1240 197 AflIII A'CryG_T Cuts at: 1203 6049 7026 1203 Size: 4846 977 1327 Fragments arranged by size: 4846 1327 977 AgeI A'CCGG_T Cuts at: 5981 5981 Size: 7150 AhdI GACnn_n'nnGTC Cuts at: 3435 5879 3435 Size: 2444 4706 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7150 AlwNI CAG_nnn'CTG Cuts at: 4893 6465 4893 Size: 1572 5578 ApaLI G'TGCA_C Cuts at: 5207 6363 5207 Size: 1156 5994 ApoI r'AATT_y Cuts at: 1493 2157 2230 2297 2461 3883 4459 4685 4837 Size: 664 73 67 164 1422 576 226 152 Cuts at: 4837 6946 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1422 664 576 226 164 152 73 67 AseI AT'TA_AT Cuts at: 160 1032 2274 4026 5704 160 Size: 872 1242 1752 1678 1606 Fragments arranged by size: 1752 1678 1606 1242 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7150 AvaI C'yCGr_G Cuts at: 1870 1943 2481 4475 1870 Size: 73 538 1994 4545 Fragments arranged by size: 4545 1994 538 73 BamHI G'GATC_C Cuts at: 2551 3632 4480 4847 2551 Size: 1081 848 367 4854 Fragments arranged by size: 4854 1081 848 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4471 5926 618 Size: 325 217 627 2684 1455 1842 Fragments arranged by size: 2684 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2672 3185 3650 4469 1391 Size: 1281 513 465 819 4072 Fragments arranged by size: 4072 1281 819 513 465 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6179 BbvCI CC'TCA_GC Cuts at: 3237 3237 Size: 7150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5341 5375 1903 Size: 34 3404 34 3678 Fragments arranged by size: 3678 3404 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5042 6252 5042 Size: 1210 5940 BclI T'GATC_A Cuts at: 1548 1548 Size: 7150 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5761 136 Size: 107 122 71 631 605 4089 1525 Fragments arranged by size: 4089 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2435 7145 2435 Size: 4710 2440 BlpI GC'TnA_GC Cuts at: 4567 4567 Size: 7150 Bme1580I G_kGCm'C Cuts at: 1790 2019 5211 6367 1790 Size: 229 3192 1156 2573 Fragments arranged by size: 3192 2573 1156 229 BmgBI CAC'GTC Cuts at: 1318 3342 1318 Size: 2024 5126 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4881 5839 442 Size: 416 53 83 399 24 3464 958 1753 Fragments arranged by size: 3464 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7150 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 3831 4152 2374 Size: 1457 321 5372 Fragments arranged by size: 5372 1457 321 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3207 3443 4161 5810 1239 Size: 1968 236 718 1649 2579 Fragments arranged by size: 2579 1968 1649 718 236 Bpu10I CC'TnA_GC Cuts at: 3237 3237 Size: 7150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2083 3634 5275 6140 6438 2083 Size: 1551 1641 865 298 2795 Fragments arranged by size: 2795 1641 1551 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5813 882 Size: 617 4314 2219 Fragments arranged by size: 4314 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4941 493 Size: 711 3737 2702 Fragments arranged by size: 3737 2702 711 BsaBI GATnn'nnATC Cuts at: 2202 3060 3164 3327 7144 2202 Size: 858 104 163 3817 2208 Fragments arranged by size: 3817 2208 858 163 104 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4325 4957 5339 5974 Size: 53 83 186 936 2792 632 382 635 Cuts at: 5974 275 Size: 1451 Fragments arranged by size: 2792 1451 936 635 632 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3336 5583 5981 6255 6402 6813 3336 Size: 2247 398 274 147 411 3673 Fragments arranged by size: 3673 2247 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4387 4417 1178 Size: 30 165 30 2984 30 3911 Fragments arranged by size: 3911 2984 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3440 4395 1186 Size: 2254 955 3941 Fragments arranged by size: 3941 2254 955 BseYI C'CCAG_C Cuts at: 6353 6849 6353 Size: 496 6654 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3339 1163 Size: 573 1603 4974 Fragments arranged by size: 4974 1603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 4600 5361 6389 1540 Size: 275 186 45 2554 761 1028 2301 Fragments arranged by size: 2554 2301 1028 761 275 186 45 BsiHKAI G_wGCw'C Cuts at: 3185 3812 5211 5296 6367 3185 Size: 627 1399 85 1071 3968 Fragments arranged by size: 3968 1399 1071 627 85 BsmI GAATG_Cn' Cuts at: 2920 4661 4754 2920 Size: 1741 93 5316 Fragments arranged by size: 5316 1741 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3480 3480 Size: 7150 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2672 3185 3650 3812 4469 5211 Size: 399 229 653 513 465 162 657 742 Cuts at: 5211 5296 6367 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 653 513 465 399 229 162 85 BspEI T'CCGG_A Cuts at: 3336 6813 3336 Size: 3477 3673 BspHI T'CATG_A Cuts at: 3085 5039 6769 3085 Size: 1954 1730 3466 Fragments arranged by size: 3466 1954 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2926 4505 844 Size: 2082 1579 3489 Fragments arranged by size: 3489 2082 1579 BsrBI CCG'CTC Cuts at: 1394 1823 5037 1394 Size: 429 3214 3507 Fragments arranged by size: 3507 3214 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5645 5819 65 Size: 1183 4397 174 1396 Fragments arranged by size: 4397 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 5794 5981 1700 Size: 110 235 3749 187 2869 Fragments arranged by size: 3749 2869 235 187 110 BsrGI T'GTAC_A Cuts at: 96 3205 96 Size: 3109 4041 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7150 BssSI C'ACGA_G Cuts at: 2884 5210 6222 2884 Size: 2326 1012 3812 Fragments arranged by size: 3812 2326 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2216 794 Size: 1422 5728 BstBI TT'CG_AA Cuts at: 5954 5954 Size: 7150 BstEII G'GTnAC_C Cuts at: 1184 2867 1184 Size: 1683 5467 BstXI CCAn_nnnn'nTGG Cuts at: 3643 3686 3721 3643 Size: 43 35 7072 Fragments arranged by size: 7072 43 35 BstYI r'GATC_y Cuts at: 1972 2203 2435 2551 3576 3632 3706 4480 4847 Size: 231 232 116 1025 56 74 774 367 Cuts at: 4847 5232 5249 6701 6787 6799 7145 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1025 774 385 367 346 232 231 116 86 74 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 513 Size: 552 939 30 5629 Fragments arranged by size: 5629 939 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6411 Fragments arranged by size: 6411 683 56 BtsI GCAGTG_nn' Cuts at: 2017 2374 2970 3714 4666 5460 5480 2017 Size: 357 596 744 952 794 20 3687 Fragments arranged by size: 3687 952 794 744 596 357 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7150 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7115 DraI TTT'AAA Cuts at: 2229 2706 4454 2229 Size: 477 1748 4925 Fragments arranged by size: 4925 1748 477 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3226 6157 809 Size: 507 1910 2931 1802 Fragments arranged by size: 2931 1910 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2578 2880 Size: 54 1425 50 275 18 213 535 302 Cuts at: 2880 4597 5486 7115 8 Size: 1717 889 1629 43 Fragments arranged by size: 1717 1629 1425 889 535 302 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2043 4597 1537 Size: 275 231 2554 4090 Fragments arranged by size: 4090 2554 275 231 EarI CTCTTCn'nnn_ Cuts at: 1353 2861 3768 5080 1353 Size: 1508 907 1312 3423 Fragments arranged by size: 3423 1508 1312 907 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5737 6111 6257 387 Size: 1064 138 4148 374 146 1280 Fragments arranged by size: 4148 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3183 3183 Size: 7150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2628 3207 3443 3878 3884 4110 4161 Size: 393 996 579 236 435 6 226 51 Cuts at: 4161 4221 4413 5213 5810 6597 6865 1239 Size: 60 192 800 597 787 268 1524 Fragments arranged by size: 1524 996 800 787 597 579 435 393 268 236 226 192 60 51 6 EcoO109I rG'GnC_Cy Cuts at: 3233 3470 3233 Size: 237 6913 EcoRI G'AATT_C Cuts at: 4459 4837 4459 Size: 378 6772 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3058 4443 3058 Size: 1385 5765 FspI TGC'GCA Cuts at: 5656 5656 Size: 7150 HaeII r_GCGC'y Cuts at: 1678 3664 6297 1678 Size: 1986 2633 2531 Fragments arranged by size: 2633 2531 1986 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3354 3361 3386 3393 3421 Size: 32 135 32 1782 7 25 7 28 Cuts at: 3421 3453 4118 4150 5791 5823 5865 5897 1373 Size: 32 665 32 1641 32 42 32 2626 Fragments arranged by size: 2626 1782 1641 665 135 42 32 32 32 32 32 32 28 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3589 3733 4494 4740 669 Size: 988 281 1651 144 761 246 3079 Fragments arranged by size: 3079 1651 988 761 281 246 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 7150 HpaI GTT'AAC Cuts at: 3733 4740 3733 Size: 1007 6143 KpnI G_GTAC'C Cuts at: 4475 4475 Size: 7150 MfeI C'AATT_G Cuts at: 4749 4749 Size: 7150 MluI A'CGCG_T Cuts at: 7026 7026 Size: 7150 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2089 2701 3788 6264 6448 1305 Size: 784 612 1087 2476 184 2007 Fragments arranged by size: 2476 2007 1087 784 612 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2580 2882 10 Size: 54 1425 343 748 302 4278 Fragments arranged by size: 4278 1425 748 343 302 54 MslI CAynn'nnrTG Cuts at: 518 1166 3026 3684 3719 5108 5467 5626 518 Size: 648 1860 658 35 1389 359 159 2042 Fragments arranged by size: 2042 1860 1389 658 648 359 159 35 MspA1I CmG'CkG Cuts at: 1606 2036 2052 4566 5243 6391 6636 1606 Size: 430 16 2514 677 1148 245 2120 Fragments arranged by size: 2514 2120 1148 677 430 245 16 NaeI GCC'GGC Cuts at: 2047 2047 Size: 7150 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5659 Fragments arranged by size: 5659 939 552 NdeI CA'TA_TG Cuts at: 387 2605 387 Size: 2218 4932 NgoMIV G'CCGG_C Cuts at: 2045 2045 Size: 7150 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7150 NotI GC'GGCC_GC Cuts at: 4597 4597 Size: 7150 NspI r_CATG'y Cuts at: 1132 1229 4508 6053 1132 Size: 97 3279 1545 2229 Fragments arranged by size: 3279 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7150 PciI A'CATG_T Cuts at: 6049 6049 Size: 7150 PflMI CCAn_nnn'nTGG Cuts at: 1108 2878 1108 Size: 1770 5380 PfoI T'CCnGG_A Cuts at: 4138 4138 Size: 7150 PmeI GTTT'AAAC Cuts at: 4454 4454 Size: 7150 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5219 5251 6732 6764 5219 Size: 32 1481 32 5605 Fragments arranged by size: 5605 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3233 3470 3233 Size: 237 6913 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7150 PsiI TTA'TAA Cuts at: 4720 4720 Size: 7150 PstI C_TGCA'G Cuts at: 830 4502 830 Size: 3672 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7150 SacI G_AGCT'C Cuts at: 3185 3185 Size: 7150 SacII CC_GC'GG Cuts at: 2037 2037 Size: 7150 SalI G'TCGA_C Cuts at: 1936 4492 1936 Size: 2556 4594 SapI GCTCTTCn'nnn_ Cuts at: 3768 3768 Size: 7150 SbfI CC_TGCA'GG Cuts at: 4502 4502 Size: 7150 ScaI AGT'ACT Cuts at: 4339 5398 7110 4339 Size: 1059 1712 4379 Fragments arranged by size: 4379 1712 1059 SexAI A'CCwGG_T Cuts at: 2398 4363 2398 Size: 1965 5185 SfcI C'TryA_G Cuts at: 826 1045 2466 2844 4498 5633 6314 6505 826 Size: 219 1421 378 1654 1135 681 191 1471 Fragments arranged by size: 1654 1471 1421 1135 681 378 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7150 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7150 SmaI CCC'GGG Cuts at: 1872 2483 4477 1872 Size: 611 1994 4545 Fragments arranged by size: 4545 1994 611 SmlI C'TyrA_G Cuts at: 820 1017 1943 2098 2257 3649 5254 6155 6417 Size: 197 926 155 159 1392 1605 901 262 Cuts at: 6417 820 Size: 1553 Fragments arranged by size: 1605 1553 1392 926 901 262 197 159 155 SnaBI TAC'GTA Cuts at: 493 4941 493 Size: 4448 2702 SpeI A'CTAG_T Cuts at: 152 2960 152 Size: 2808 4342 SphI G_CATG'C Cuts at: 1132 4508 1132 Size: 3376 3774 SspI AAT'ATT Cuts at: 5 52 5074 5 Size: 47 5022 2081 Fragments arranged by size: 5022 2081 47 StuI AGG'CCT Cuts at: 1517 3949 1517 Size: 2432 4718 StyI C'CwwG_G Cuts at: 513 1065 2004 3656 4297 4589 513 Size: 552 939 1652 641 292 3074 Fragments arranged by size: 3074 1652 939 641 552 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2236 2825 3148 3228 3932 5177 5194 Size: 1255 656 589 323 80 704 1245 17 Cuts at: 5194 5347 5532 5977 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1245 704 656 589 445 323 185 153 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3205 3593 4337 Size: 274 80 33 51 1443 1228 388 744 Cuts at: 4337 4440 5396 7108 96 Size: 103 956 1712 138 Fragments arranged by size: 1712 1443 1228 956 744 388 274 138 103 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2532 2564 4170 4202 7110 7142 2532 Size: 32 1606 32 2908 32 2540 Fragments arranged by size: 2908 2540 1606 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7150 XbaI T'CTAG_A Cuts at: 4486 4486 Size: 7150 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2679 2679 Size: 7150 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7150 XmaI C'CCGG_G Cuts at: 1870 2481 4475 1870 Size: 611 1994 4545 Fragments arranged by size: 4545 1994 611 XmnI GAAnn'nnTTC Cuts at: 5279 5279 Size: 7150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4958 276 Size: 53 83 186 936 3424 2468 Fragments arranged by size: 3424 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SfiI SfoI SrfI SwaI