(Linear) (Six-base) MAPSORT of: KIEE4231.seq Check: 3418 from: 1 to: 2445 KIEE4231.orf With 182 enzymes: SgfI * March 22, 2012 17:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 623 1873 1879 2105 2216 2408 2445 Size: 623 1250 6 226 111 192 37 Fragments arranged by size: 1250 623 226 192 111 37 6 AflII C'TTAA_G Cuts at: 0 252 2445 Size: 252 2193 AhdI GACnn_n'nnGTC Cuts at: 0 1430 2445 Size: 1430 1015 ApoI r'AATT_y Cuts at: 0 152 225 292 456 1878 2445 Size: 152 73 67 164 1422 567 Fragments arranged by size: 1422 567 164 152 73 67 AseI AT'TA_AT Cuts at: 0 269 2021 2445 Size: 269 1752 424 Fragments arranged by size: 1752 424 269 AvaI C'yCGr_G Cuts at: 0 476 2445 Size: 476 1969 BamHI G'GATC_C Cuts at: 0 546 1627 2445 Size: 546 1081 818 Fragments arranged by size: 1081 818 546 BanII G_rGCy'C Cuts at: 0 667 1180 1645 2445 Size: 667 513 465 800 Fragments arranged by size: 800 667 513 465 BbvCI CC'TCA_GC Cuts at: 0 1232 2445 Size: 1232 1213 BglII A'GATC_T Cuts at: 0 430 2445 Size: 430 2015 Bme1580I G_kGCm'C Cuts at: 0 14 2445 Size: 14 2431 BmgBI CAC'GTC Cuts at: 0 1337 2445 Size: 1337 1108 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 369 1826 2147 2445 Size: 369 1457 321 298 Fragments arranged by size: 1457 369 321 298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1202 1438 2156 2445 Size: 1202 236 718 289 Fragments arranged by size: 1202 718 289 236 Bpu10I CC'TnA_GC Cuts at: 0 1232 2445 Size: 1232 1213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 1629 2445 Size: 78 1551 816 Fragments arranged by size: 1551 816 78 BsaBI GATnn'nnATC Cuts at: 0 197 1055 1159 1322 2445 Size: 197 858 104 163 1123 Fragments arranged by size: 1123 858 197 163 104 BsaHI Gr'CG_yC Cuts at: 0 2320 2445 Size: 2320 125 BsaWI w'CCGG_w Cuts at: 0 1331 2445 Size: 1331 1114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2382 2412 2445 Size: 2382 30 33 Fragments arranged by size: 2382 33 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1435 2390 2445 Size: 1435 955 55 Fragments arranged by size: 1435 955 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1334 2445 Size: 1334 1111 BsiEI CG_ry'CG Cuts at: 0 41 2445 Size: 41 2404 BsiHKAI G_wGCw'C Cuts at: 0 1180 1807 2445 Size: 1180 627 638 Fragments arranged by size: 1180 638 627 BsmI GAATG_Cn' Cuts at: 0 915 2445 Size: 915 1530 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1475 2445 Size: 1475 970 Bsp1286I G_dGCh'C Cuts at: 0 14 667 1180 1645 1807 2445 Size: 14 653 513 465 162 638 Fragments arranged by size: 653 638 513 465 162 14 BspEI T'CCGG_A Cuts at: 0 1331 2445 Size: 1331 1114 BspHI T'CATG_A Cuts at: 0 1080 2445 Size: 1080 1365 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 921 2445 Size: 921 1524 BsrFI r'CCGG_y Cuts at: 0 40 2445 Size: 40 2405 BsrGI T'GTAC_A Cuts at: 0 1200 2445 Size: 1200 1245 BssSI C'ACGA_G Cuts at: 0 879 2445 Size: 879 1566 BstAPI GCAn_nnn'nTGC Cuts at: 0 211 2445 Size: 211 2234 BstEII G'GTnAC_C Cuts at: 0 862 2445 Size: 862 1583 BstXI CCAn_nnnn'nTGG Cuts at: 0 1638 1681 1716 2445 Size: 1638 43 35 729 Fragments arranged by size: 1638 729 43 35 BstYI r'GATC_y Cuts at: 0 198 430 546 1571 1627 1701 2445 Size: 198 232 116 1025 56 74 744 Fragments arranged by size: 1025 744 232 198 116 74 56 BtgI C'CryG_G Cuts at: 0 29 2445 Size: 29 2416 BtsI GCAGTG_nn' Cuts at: 0 12 369 965 1709 2445 Size: 12 357 596 744 736 Fragments arranged by size: 744 736 596 357 12 DraI TTT'AAA Cuts at: 0 224 701 2445 Size: 224 477 1744 Fragments arranged by size: 1744 477 224 DrdI GACnn_nn'nnGTC Cuts at: 0 1221 2445 Size: 1221 1224 EaeI y'GGCC_r Cuts at: 0 38 573 875 2445 Size: 38 535 302 1570 Fragments arranged by size: 1570 535 302 38 EagI C'GGCC_G Cuts at: 0 38 2445 Size: 38 2407 EarI CTCTTCn'nnn_ Cuts at: 0 856 1763 2445 Size: 856 907 682 Fragments arranged by size: 907 856 682 EcoICRI GAG'CTC Cuts at: 0 1178 2445 Size: 1178 1267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 623 1202 1438 1873 1879 2105 2156 2216 Size: 623 579 236 435 6 226 51 60 Cuts at: 2216 2408 2445 Size: 192 37 Fragments arranged by size: 623 579 435 236 226 192 60 51 37 6 EcoO109I rG'GnC_Cy Cuts at: 0 1228 1465 2445 Size: 1228 237 980 Fragments arranged by size: 1228 980 237 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1053 2438 2445 Size: 1053 1385 7 Fragments arranged by size: 1385 1053 7 HaeII r_GCGC'y Cuts at: 0 1659 2445 Size: 1659 786 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1349 1356 1381 1388 1416 1448 2113 2145 Size: 1349 7 25 7 28 32 665 32 Cuts at: 2145 2445 Size: 300 Fragments arranged by size: 1349 665 300 32 32 28 25 7 7 HincII GTy'rAC Cuts at: 0 1584 1728 2445 Size: 1584 144 717 Fragments arranged by size: 1584 717 144 HpaI GTT'AAC Cuts at: 0 1728 2445 Size: 1728 717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 84 696 1783 2445 Size: 84 612 1087 662 Fragments arranged by size: 1087 662 612 84 MscI TGG'CCA Cuts at: 0 575 877 2445 Size: 575 302 1568 Fragments arranged by size: 1568 575 302 MslI CAynn'nnrTG Cuts at: 0 1021 1679 1714 2445 Size: 1021 658 35 731 Fragments arranged by size: 1021 731 658 35 MspA1I CmG'CkG Cuts at: 0 31 47 2445 Size: 31 16 2398 Fragments arranged by size: 2398 31 16 NaeI GCC'GGC Cuts at: 0 42 2445 Size: 42 2403 NdeI CA'TA_TG Cuts at: 0 600 2445 Size: 600 1845 NgoMIV G'CCGG_C Cuts at: 0 40 2445 Size: 40 2405 PflMI CCAn_nnn'nTGG Cuts at: 0 873 2445 Size: 873 1572 PfoI T'CCnGG_A Cuts at: 0 2133 2445 Size: 2133 312 PpuMI rG'GwC_Cy Cuts at: 0 1228 1465 2445 Size: 1228 237 980 Fragments arranged by size: 1228 980 237 SacI G_AGCT'C Cuts at: 0 1180 2445 Size: 1180 1265 SacII CC_GC'GG Cuts at: 0 32 2445 Size: 32 2413 SapI GCTCTTCn'nnn_ Cuts at: 0 1763 2445 Size: 1763 682 ScaI AGT'ACT Cuts at: 0 2334 2445 Size: 2334 111 SexAI A'CCwGG_T Cuts at: 0 393 2358 2445 Size: 393 1965 87 Fragments arranged by size: 1965 393 87 SfcI C'TryA_G Cuts at: 0 461 839 2445 Size: 461 378 1606 Fragments arranged by size: 1606 461 378 SmaI CCC'GGG Cuts at: 0 478 2445 Size: 478 1967 SmlI C'TyrA_G Cuts at: 0 93 252 1644 2445 Size: 93 159 1392 801 Fragments arranged by size: 1392 801 159 93 SpeI A'CTAG_T Cuts at: 0 955 2445 Size: 955 1490 StuI AGG'CCT Cuts at: 0 1944 2445 Size: 1944 501 StyI C'CwwG_G Cuts at: 0 1651 2292 2445 Size: 1651 641 153 Fragments arranged by size: 1651 641 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 231 820 1143 1223 1927 2445 Size: 231 589 323 80 704 518 Fragments arranged by size: 704 589 518 323 231 80 TatI w'GTAC_w Cuts at: 0 1200 1588 2332 2435 2445 Size: 1200 388 744 103 10 Fragments arranged by size: 1200 744 388 103 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 527 559 2165 2197 2445 Size: 527 32 1606 32 248 Fragments arranged by size: 1606 527 248 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 674 2445 Size: 674 1771 XmaI C'CCGG_G Cuts at: 0 476 2445 Size: 476 1969 Enzymes that do cut: AcuI AflII AhdI ApoI AseI AvaI BamHI BanII BbvCI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DraI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV PflMI PfoI PpuMI SacI SacII SapI ScaI SexAI SfcI SmaI SmlI SpeI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BanI BbeI BbsI BcgI BciVI BclI BfrBI BglI BlpI BmrI BmtI BsaI BsaAI BseYI BsiWI BsrBI BsrDI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EciI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI NarI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI