Restriction map for
pFN21AE0906
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I EarI Eco47III EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AfaI 448 GTAC GT^AC
AocI 383 CCTCAGG CC^T[ACGT]AGG
AspHI 413 GAGCTC G[AT]GC[AT]^C
AvaII 334 GGTCC G^G[AT]CC
BalI 339 TGGCCA TGG^CCA
BanI 21 GGTGCC G^G[CT][GA]CC
BanII 413 GAGCTC G[GA]GC[CT]^C
BmyI 413 GAGCTC G[AGT]GC[ACT]^C
BpmI 184 CTGGAG CTGGAG(16/14)
BseRI 202 GAGGAG GAGGAG(10/8)
BsiEI 232 CGGCCG CG[GA][CT]^CG
BsiHKAI 413 GAGCTC G[AT]GC[AT]^C
Bsp1286I 413 GAGCTC G[AGT]GC[ACT]^C
BstF5I 523 CATCC GGATG(2/0)
BstYI 149 AGATCC [GA]^GATC[CT]
Bsu36I 383 CCTCAGG CC^T[ACGT]AGG
CfoI 576 GCGC GCG^C
EagI 232 CGGCCG C^GGCCG
EclXI 232 CGGCCG C^GGCCG
Eco52I 232 CGGCCG C^GGCCG
Eco81I 383 CCTCAGG CC^T[ACGT]AGG
EcoT14I 272 CCTTGG C^C[AT][AT]GG
EcoT22I 520 ATGCAT ATGCA^T
FauI 119 CCCGC CCCGC(4/6)
FokI 523 CATCC GGATG(9/13)
HapII 231 CCGG C^CGG
HhaI 576 GCGC GCG^C
HinP1I 576 GCGC G^CGC
HincII 467 GTCAAC GT[CT]^[GA]AC
HindII 467 GTCAAC GT[CT]^[GA]AC
HindIII 589 AAGCTT A^AGCTT
HinfI 211 GAATC G^A[ACGT]TC
HpaII 231 CCGG C^CGG
HphI 582 GGTGA GGTGA(8/7)
MflI 149 AGATCC [GA]^GATC[CT]
MluNI 339 TGGCCA TGG^CCA
MscI 339 TGGCCA TGG^CCA
MseI 251 TTAA T^TAA
MspI 231 CCGG C^CGG
NsiI 520 ATGCAT ATGCA^T
PflMI 355 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 625 CTGCAG CTGCA^G
PvuII 281 CAGCTG CAG^CTG
RsaI 448 GTAC GT^AC
SacI 413 GAGCTC GAGCT^C
SfiI 340 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 272 CCTTGG C^C[AT][AT]GG
TfiI 211 GAATC G^A[AT]TC
Tru9I 251 TTAA T^TAA
Tsp509I 310 AATT ^AATT
Van91I 355 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 351 CCAGCCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 149 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 150 GATCC GGATC(4/5)
369 GATCC
AlwNI 110 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
356 CAGGAGCTG
BglI 271 GCCTTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
341 GCCAGGCAGGC
BsaI 315 GAGACC GGTCTC(1/5)
226 GGTCTC
BsgI 128 GTGCAG GTGCAG(16/14)
400 GTGCAG
BsmAI 315 GAGAC GTCTC(1/5)
227 GTCTC
BsmFI 364 GGGAC GGGAC(10/14)
46 GTCCC
BstAPI 280 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
429 GCAGCCAATGC
DpnII 150 GATC ^GATC
369 GATC
EaeI 232 CGGCCG [CT]^GGCC[GA]
339 TGGCCA
MboI 150 GATC ^GATC
369 GATC
MboII 85 GAAGA GAAGA(8/7)
585 GAAGA
MspA1I 281 CAGCTG C[AC]G^C[GT]G
532 CAGCGG
NdeII 150 GATC ^GATC
369 GATC
NlaIII 37 CATG CATG^
519 CATG
NlaIV 21 GGTGCC GG[ACGT]^[ACGT]CC
379 GGAACC
Sau3AI 150 GATC ^GATC
369 GATC
SfcI 257 CTACAG C^T[GA][CT]AG
625 CTGCAG
TspRI 330 CAGTG CA[GC]TG[ACGT][ACGT]^
441 CAGTG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BspMI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI HaeIII
ItaI MnlI MvaI MwoI Sau96I
ScrFI SfaNI TseI