Restriction map for
pFN21AE0906
(Circular) (Six-base) MAPSORT of: pFN21AE0906.seq Check: 6971 from: 1 to: 5341
pFN21AE0906 5341 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2392 2637 2392
Size: 245 5096
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 2103 2310 3404 4788 5056 1632
Size: 405 66 207 1094 1384 268 1917
Fragments arranged by size:
1917 1384 1094 405 268 207 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 2120 2366 3084 4656 2120
Size: 246 718 1572 2805
Fragments arranged by size:
2805 1572 718 246
ApaLI G'TGCA_C
Cuts at: 3398 4554 3398
Size: 1156 4185
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2662 4117 618
Size: 325 217 627 239 636 1455 1842
Fragments arranged by size:
1842 1455 636 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2422 2660 1391
Size: 1031 238 4072
Fragments arranged by size:
4072 1031 238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3532 3566 1903
Size: 34 50 34 1511 34 3678
Fragments arranged by size:
3678 1511 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BfrBI ATG'CAT
Cuts at: 2527 2527
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 2352 3952
Size: 107 122 71 631 605 610 70 1600
Cuts at: 3952 136
Size: 1525
Fragments arranged by size:
1600 1525 631 610 605 122 107 71
70
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 3402 4558 1790
Size: 1612 1156 2573
Fragments arranged by size:
2573 1612 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 4001 1239
Size: 971 1791 2579
Fragments arranged by size:
2579 1791 971
Bpu10I CC'TnA_GC
Cuts at: 2463 2463
Size: 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4331 4629 3466
Size: 865 298 4178
Fragments arranged by size:
4178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 2314 4004 882
Size: 617 738 77 1690 2219
Fragments arranged by size:
2219 1690 738 617 77
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5116
Fragments arranged by size:
5116 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 1186
Size: 1036 4305
BseYI C'CCAG_C
Cuts at: 2366 4544 5040 2366
Size: 2178 496 2667
Fragments arranged by size:
2667 2178 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2154 2426 1163
Size: 573 418 272 4078
Fragments arranged by size:
4078 573 418 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2240 2791 3552 4580 1540
Size: 275 186 239 551 761 1028 2301
Fragments arranged by size:
2301 1028 761 551 275 239 186
BsiHKAI G_wGCw'C
Cuts at: 2422 3402 3487 4558 2422
Size: 980 85 1071 3205
Fragments arranged by size:
3205 1071 980 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2422 2660 3402 3487 4558 1391
Size: 399 632 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 632 399 238 85
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2392 2459 2637 2696 844
Size: 1548 67 178 59 3489
Fragments arranged by size:
3489 1548 178 67 59
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3985 4172 1700
Size: 110 2175 187 2869
Fragments arranged by size:
2869 2175 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2291 2440 794
Size: 1497 149 3695
Fragments arranged by size:
3695 1497 149
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2154 2671 3038 3423 3440 4892 4978 4990
Size: 182 517 367 385 17 1452 86 12
Cuts at: 4990 5336 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 517 385 367 346 182 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2389 2389
Size: 5341
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4789
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 2857 3651 3671 2341
Size: 516 794 20 4011
Fragments arranged by size:
4011 794 516 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 2344 2788
Size: 54 1425 50 275 18 407 107 444
Cuts at: 2788 3677 5306 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 444 407 275 107 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2237 2788 1537
Size: 275 425 551 4090
Fragments arranged by size:
4090 551 425 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3271 1353
Size: 1918 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2420 2420
Size: 5341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2103 2210 2310 3404 4001 4788
Size: 393 405 66 107 100 1094 597 787
Cuts at: 4788 5056 1239
Size: 268 1524
Fragments arranged by size:
1524 1094 787 597 405 393 268 107
100 66
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 5341
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 4488 1678
Size: 2810 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2215 2233 2247 3982
Size: 32 135 32 629 14 18 14 1735
Cuts at: 3982 4014 4056 4088 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1735 629 135 42 32 32 32
32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2474 2685 2931 669
Size: 988 281 536 211 246 3079
Fragments arranged by size:
3079 988 536 281 246 211
HindIII A'AGCT_T
Cuts at: 748 2594 748
Size: 1846 3495
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2346 10
Size: 54 1425 343 514 3005
Fragments arranged by size:
3005 1425 514 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3299 3658 3817 518
Size: 648 2133 359 159 2042
Fragments arranged by size:
2133 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2288 2539 2757 3434 4582 4827 1606
Size: 682 251 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 682 677 251 245 218
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4789
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NsiI A_TGCA'T
Cuts at: 2529 2529
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2366 1108
Size: 1258 4083
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2634 2693 830
Size: 1804 59 3478
Fragments arranged by size:
3478 1804 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
PvuII CAG'CTG
Cuts at: 2288 2288
Size: 5341
SacI G_AGCT'C
Cuts at: 2422 2422
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SfcI C'TryA_G
Cuts at: 826 1045 2262 2630 2689 3824 4505 4696 826
Size: 219 1217 368 59 1135 681 191 1471
Fragments arranged by size:
1471 1217 1135 681 368 219 191 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 2352 2352
Size: 5341
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3445 4346 4608 820
Size: 197 926 1502 901 262 1553
Fragments arranged by size:
1553 1502 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2277 2780 513
Size: 552 1212 503 3074
Fragments arranged by size:
3074 1212 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2363 2363
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfoI SrfI SwaI