Restriction map for
pFN21AE0906
(Linear) (Six-base) MAPSORT of: KIEE0906.seq Check: 7482 from: 1 to: 636
KIEE0906.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 387 632 636
Size: 387 245 4
Fragments arranged by size:
387 245 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 98 305 636
Size: 32 66 207 331
Fragments arranged by size:
331 207 66 32
AlwNI CAG_nnn'CTG
Cuts at: 0 115 361 636
Size: 115 246 275
Fragments arranged by size:
275 246 115
BanI G'GyrC_C
Cuts at: 0 21 636
Size: 21 615
BanII G_rGCy'C
Cuts at: 0 417 636
Size: 417 219
BfrBI ATG'CAT
Cuts at: 0 522 636
Size: 522 114
BglI GCCn_nnn'nGGC
Cuts at: 0 277 347 636
Size: 277 70 289
Fragments arranged by size:
289 277 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 636
Size: 205 431
Bpu10I CC'TnA_GC
Cuts at: 0 458 636
Size: 458 178
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 309 636
Size: 232 77 327
Fragments arranged by size:
327 232 77
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 636
Size: 217 419
BseYI C'CCAG_C
Cuts at: 0 361 636
Size: 361 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 421 636
Size: 149 272 215
Fragments arranged by size:
272 215 149
BsiEI CG_ry'CG
Cuts at: 0 235 636
Size: 235 401
BsiHKAI G_wGCw'C
Cuts at: 0 417 636
Size: 417 219
Bsp1286I G_dGCh'C
Cuts at: 0 417 636
Size: 417 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 387 454 632 636
Size: 387 67 178 4
Fragments arranged by size:
387 178 67 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 286 435 636
Size: 286 149 201
Fragments arranged by size:
286 201 149
BstYI r'GATC_y
Cuts at: 0 149 636
Size: 149 487
Bsu36I CC'TnA_GG
Cuts at: 0 384 636
Size: 384 252
BtsI GCAGTG_nn'
Cuts at: 0 336 636
Size: 336 300
EaeI y'GGCC_r
Cuts at: 0 232 339 636
Size: 232 107 297
Fragments arranged by size:
297 232 107
EagI C'GGCC_G
Cuts at: 0 232 636
Size: 232 404
EcoICRI GAG'CTC
Cuts at: 0 415 636
Size: 415 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 98 205 305 636
Size: 32 66 107 100 331
Fragments arranged by size:
331 107 100 66 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 636
Size: 264 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 210 228 242 636
Size: 196 14 18 14 394
Fragments arranged by size:
394 196 18 14 14
HincII GTy'rAC
Cuts at: 0 469 636
Size: 469 167
HindIII A'AGCT_T
Cuts at: 0 589 636
Size: 589 47
MscI TGG'CCA
Cuts at: 0 341 636
Size: 341 295
MspA1I CmG'CkG
Cuts at: 0 283 534 636
Size: 283 251 102
Fragments arranged by size:
283 251 102
NsiI A_TGCA'T
Cuts at: 0 524 636
Size: 524 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 361 636
Size: 361 275
PstI C_TGCA'G
Cuts at: 0 629 636
Size: 629 7
PvuII CAG'CTG
Cuts at: 0 283 636
Size: 283 353
SacI G_AGCT'C
Cuts at: 0 417 636
Size: 417 219
SfcI C'TryA_G
Cuts at: 0 257 625 636
Size: 257 368 11
Fragments arranged by size:
368 257 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 347 636
Size: 347 289
StyI C'CwwG_G
Cuts at: 0 272 636
Size: 272 364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 358 636
Size: 358 278
Enzymes that do cut:
AarI AcuI AlwNI BanI BanII BfrBI BglI BpmI
Bpu10I BsaI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BstAPI BstYI Bsu36I BtsI EaeI EagI EcoICRI
Eco57MI FalI Hin4I HincII HindIII MscI MspA1I NsiI
PflMI PstI PvuII SacI SfcI SfiI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI