(Circular) (Six-base) MAPSORT of: pFN21AE0903.seq Check: 4499 from: 1 to: 5209 pFN21AE0903 5209 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3019 278 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2552 1937 Size: 615 4594 Acc65I G'GTAC_C Cuts at: 2530 2530 Size: 5209 AclI AA'CG_TT Cuts at: 1563 3336 3709 1563 Size: 1773 373 3063 Fragments arranged by size: 3063 1773 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2180 2397 3272 4656 4924 1632 Size: 462 86 217 875 1384 268 1917 Fragments arranged by size: 1917 1384 875 462 268 217 86 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5012 AflIII A'CryG_T Cuts at: 1203 4108 5085 1203 Size: 2905 977 1327 Fragments arranged by size: 2905 1327 977 AgeI A'CCGG_T Cuts at: 4040 4040 Size: 5209 AhdI GACnn_n'nnGTC Cuts at: 3938 3938 Size: 5209 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5209 AlwNI CAG_nnn'CTG Cuts at: 2952 4524 2952 Size: 1572 3637 ApaLI G'TGCA_C Cuts at: 3266 4422 3266 Size: 1156 4053 ApoI r'AATT_y Cuts at: 1493 2214 2435 2518 2744 2896 5005 1493 Size: 721 221 83 226 152 2109 1697 Fragments arranged by size: 2109 1697 721 226 221 152 83 AseI AT'TA_AT Cuts at: 160 1032 3763 160 Size: 872 2731 1606 Fragments arranged by size: 2731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 AvaI C'yCGr_G Cuts at: 1870 1943 2534 1870 Size: 73 591 4545 Fragments arranged by size: 4545 591 73 BamHI G'GATC_C Cuts at: 2539 2906 2539 Size: 367 4842 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2530 3985 618 Size: 325 217 627 233 510 1455 1842 Fragments arranged by size: 1842 1455 627 510 325 233 217 BanII G_rGCy'C Cuts at: 1391 2528 1391 Size: 1137 4072 BbeI G_GCGC'C Cuts at: 2024 2024 Size: 5209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 928 Size: 971 159 4079 Fragments arranged by size: 4079 971 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3400 3434 1903 Size: 34 1463 34 3678 Fragments arranged by size: 3678 1463 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3101 4311 3101 Size: 1210 3999 BclI T'GATC_A Cuts at: 1548 1548 Size: 5209 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3820 136 Size: 107 122 71 631 605 2148 1525 Fragments arranged by size: 2148 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2191 5204 2191 Size: 3013 2196 BlpI GC'TnA_GC Cuts at: 2626 2626 Size: 5209 Bme1580I G_kGCm'C Cuts at: 1790 3270 4426 1790 Size: 1480 1156 2573 Fragments arranged by size: 2573 1480 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5209 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2940 3898 442 Size: 416 53 83 399 24 1523 958 1753 Fragments arranged by size: 1753 1523 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2391 1056 Size: 1335 3874 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3869 1239 Size: 2630 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3334 4199 4497 3334 Size: 865 298 4046 Fragments arranged by size: 4046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3872 882 Size: 617 2373 2219 Fragments arranged by size: 2373 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3000 493 Size: 711 1796 2702 Fragments arranged by size: 2702 1796 711 BsaBI GATnn'nnATC Cuts at: 5203 5203 Size: 5209 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 3016 3398 4033 Size: 53 83 186 936 488 995 382 635 Cuts at: 4033 275 Size: 1451 Fragments arranged by size: 1451 995 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2150 3642 4040 4314 4461 4872 2150 Size: 1492 398 274 147 411 2487 Fragments arranged by size: 2487 1492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4984 Fragments arranged by size: 4984 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5209 BseYI C'CCAG_C Cuts at: 4412 4908 4412 Size: 496 4713 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4636 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2659 3420 4448 1540 Size: 275 186 658 761 1028 2301 Fragments arranged by size: 2301 1028 761 658 275 186 BsiHKAI G_wGCw'C Cuts at: 3270 3355 4426 3270 Size: 85 1071 4053 Fragments arranged by size: 4053 1071 85 BsmI GAATG_Cn' Cuts at: 2720 2813 2720 Size: 93 5116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2528 3270 3355 4426 1391 Size: 399 738 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 738 399 85 BspEI T'CCGG_A Cuts at: 4872 4872 Size: 5209 BspHI T'CATG_A Cuts at: 3098 4828 3098 Size: 1730 3479 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2564 844 Size: 1720 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3096 1394 Size: 429 1273 3507 Fragments arranged by size: 3507 1273 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2237 3704 3878 65 Size: 1183 989 1467 174 1396 Fragments arranged by size: 1467 1396 1183 989 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 3853 4040 1700 Size: 110 207 1836 187 2869 Fragments arranged by size: 2869 1836 207 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5209 BssSI C'ACGA_G Cuts at: 3269 4281 3269 Size: 1012 4197 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5209 BstBI TT'CG_AA Cuts at: 4013 4013 Size: 5209 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5209 BstYI r'GATC_y Cuts at: 1972 2191 2407 2467 2539 2906 3291 3308 4760 Size: 219 216 60 72 367 385 17 1452 Cuts at: 4760 4846 4858 5204 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 219 216 86 72 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3718 Fragments arranged by size: 3718 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4470 Fragments arranged by size: 4470 683 56 BtsI GCAGTG_nn' Cuts at: 2725 3519 3539 2725 Size: 794 20 4395 Fragments arranged by size: 4395 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5209 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5174 DraI TTT'AAA Cuts at: 2131 2513 2131 Size: 382 4827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4216 809 Size: 507 2900 1802 Fragments arranged by size: 2900 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2656 3545 5174 Size: 54 1425 50 275 18 826 889 1629 Cuts at: 5174 8 Size: 43 Fragments arranged by size: 1629 1425 889 826 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2656 1537 Size: 275 844 4090 Fragments arranged by size: 4090 844 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3139 1353 Size: 1786 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3796 4170 4316 387 Size: 1064 138 2207 374 146 1280 Fragments arranged by size: 2207 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2180 2397 3272 3869 4656 4924 Size: 393 462 86 217 875 597 787 268 Cuts at: 4924 1239 Size: 1524 Fragments arranged by size: 1524 875 787 597 462 393 268 217 86 EcoRI G'AATT_C Cuts at: 2435 2518 2896 2435 Size: 83 378 4748 Fragments arranged by size: 4748 378 83 FspI TGC'GCA Cuts at: 3715 3715 Size: 5209 HaeII r_GCGC'y Cuts at: 1678 2024 2316 4356 1678 Size: 346 292 2040 2531 Fragments arranged by size: 2531 2040 346 292 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2392 2424 2459 2491 3850 Size: 32 135 32 820 32 35 32 1359 Cuts at: 3850 3882 3924 3956 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1359 820 135 42 35 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2553 2799 669 Size: 988 281 615 246 3079 Fragments arranged by size: 3079 988 615 281 246 HindIII A'AGCT_T Cuts at: 748 2099 748 Size: 1351 3858 HpaI GTT'AAC Cuts at: 2799 2799 Size: 5209 KasI G'GCGC_C Cuts at: 2020 2020 Size: 5209 KpnI G_GTAC'C Cuts at: 2534 2534 Size: 5209 MfeI C'AATT_G Cuts at: 2808 2808 Size: 5209 MluI A'CGCG_T Cuts at: 5085 5085 Size: 5209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4323 4507 1305 Size: 3018 184 2007 Fragments arranged by size: 3018 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3387 Fragments arranged by size: 3387 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3167 3526 3685 518 Size: 648 2001 359 159 2042 Fragments arranged by size: 2042 2001 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2625 3302 4450 4695 1606 Size: 1019 677 1148 245 2120 Fragments arranged by size: 2120 1148 1019 677 245 NaeI GCC'GGC Cuts at: 2019 2019 Size: 5209 NarI GG'CG_CC Cuts at: 2021 2021 Size: 5209 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3718 Fragments arranged by size: 3718 939 552 NdeI CA'TA_TG Cuts at: 387 2245 387 Size: 1858 3351 NgoMIV G'CCGG_C Cuts at: 2017 2017 Size: 5209 NheI G'CTAG_C Cuts at: 1052 2387 1052 Size: 1335 3874 NotI GC'GGCC_GC Cuts at: 2656 2656 Size: 5209 NspI r_CATG'y Cuts at: 1132 1229 2567 4112 1132 Size: 97 1338 1545 2229 Fragments arranged by size: 2229 1545 1338 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5209 PciI A'CATG_T Cuts at: 4108 4108 Size: 5209 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5209 PmeI GTTT'AAAC Cuts at: 2513 2513 Size: 5209 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3278 3310 4791 4823 3278 Size: 32 1481 32 3664 Fragments arranged by size: 3664 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5209 PsiI TTA'TAA Cuts at: 2779 2779 Size: 5209 PstI C_TGCA'G Cuts at: 830 2561 830 Size: 1731 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5209 SalI G'TCGA_C Cuts at: 1936 2551 1936 Size: 615 4594 SbfI CC_TGCA'GG Cuts at: 2561 2561 Size: 5209 ScaI AGT'ACT Cuts at: 2258 2306 3457 5169 2258 Size: 48 1151 1712 2298 Fragments arranged by size: 2298 1712 1151 48 SfcI C'TryA_G Cuts at: 826 1045 2557 3692 4373 4564 826 Size: 219 1512 1135 681 191 1471 Fragments arranged by size: 1512 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2022 2022 Size: 5209 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 SgrAI Cr'CCGG_yG Cuts at: 1700 2017 1700 Size: 317 4892 SmaI CCC'GGG Cuts at: 1872 2536 1872 Size: 664 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3313 4214 4476 820 Size: 197 926 1370 901 262 1553 Fragments arranged by size: 1553 1370 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3000 493 Size: 2507 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5209 SphI G_CATG'C Cuts at: 1132 2567 1132 Size: 1435 3774 SspI AAT'ATT Cuts at: 5 52 3133 5 Size: 47 3081 2081 Fragments arranged by size: 3081 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5209 StyI C'CwwG_G Cuts at: 513 1065 2004 2648 513 Size: 552 939 644 3074 Fragments arranged by size: 3074 939 644 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3236 3253 3406 3591 4036 325 Size: 1255 1656 17 153 185 445 1498 Fragments arranged by size: 1656 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 2304 3455 Size: 274 80 33 51 1443 279 48 1151 Cuts at: 3455 5167 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1151 279 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2132 2164 5169 5201 2132 Size: 32 3005 32 2140 Fragments arranged by size: 3005 2140 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5209 XbaI T'CTAG_A Cuts at: 2545 2545 Size: 5209 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5209 XmaI C'CCGG_G Cuts at: 1870 2534 1870 Size: 664 4545 XmnI GAAnn'nnTTC Cuts at: 3338 3338 Size: 5209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3017 276 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI