Restriction map for
pFN21AE4164
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
BspDI BspEI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
FauI FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeII MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI Sau96I SbfI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
StuI Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1699 GTCTAC GT^[AC][GT]AC
AciI 961 CCGC CCGC(-3/-1)
AflII 102 CTTAAG C^TTAAG
AflIII 34 ACATGT A^C[GA][CT]GT
Asp700I 1014 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1232 CCCGGG C^[CT]CG[GA]G
BamHI 1228 GGATCC G^GATCC
BbvI 199 GCAGC GCAGC(8/12)
BfrI 102 CTTAAG C^TTAAG
BsaI 1796 GAGACC GGTCTC(1/5)
BseRI 730 GAGGAG GAGGAG(10/8)
BsiYI 1280 CCAAAGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1280 CCAAAGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1235 GGGAC GGGAC(10/14)
BsoBI 1232 CCCGGG C^[CT]CG[GA]G
BspHI 1086 TCATGA T^CATGA
BspLU11I 34 ACATGT A^CATGT
DsaI 1801 CCATGG C^C[GA][CT]GG
EcoRI 44 GAATTC G^AATTC
EcoRV 1290 GATATC GAT^ATC
HapII 1233 CCGG C^CGG
HindIII 404 AAGCTT A^AGCTT
HpaII 1233 CCGG C^CGG
MspI 1233 CCGG C^CGG
MwoI 292 GCAGTAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1801 CCATGG C^CATGG
NdeI 11 CATATG CA^TATG
PacI 1489 TTAATTAA TTAAT^TAA
PflMI 1280 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1086 TCATGA T^CATGA
ScaI 576 AGTACT AGT^ACT
SexAI 1105 ACCTGGT A^CC[AT]GGT
SmaI 1232 CCCGGG CCC^GGG
SmlI 102 CTTAAG C^T[CT][GA]AG
SpeI 1452 ACTAGT A^CTAGT
SphI 202 GCATGC GCATG^C
SspI 1017 AATATT AAT^ATT
SwaI 540 ATTTAAAT ATTT^AAAT
TseI 199 GCAGC G^C[AT]GC
Van91I 1280 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1232 CCCGGG C^CCGGG
XmaI 1232 CCCGGG C^CCGGG
XmnI 1014 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 433 ATTAAT AT^TAAT
1488 ATTAAT
AsnI 433 ATTAAT AT^TAAT
1488 ATTAAT
BcnI 1232 CCCGG CC^[GC]GG
1233 CCGGG
BsgI 1604 GTGCAG GTGCAG(16/14)
1693 GTGCAG
Bsp1407I 516 TGTACA T^GTACA
1513 TGTACA
BspMI 1618 GCAGGT ACCTGC(4/8)
1695 GCAGGT
BsrGI 516 TGTACA T^GTACA
1513 TGTACA
BstF5I 857 CATCC GGATG(2/0)
804 GGATG
DraI 541 TTTAAA TTT^AAA
1417 TTTAAA
EarI 838 CTCTTC CTCTTC(1/4)
760 GAAGAG
EcoT14I 287 CCAAGG C^C[AT][AT]GG
1801 CCATGG
EcoT22I 503 ATGCAT ATGCA^T
1171 ATGCAT
Fnu4HI 199 GCAGC GC^[ACGT]GC
960 GCCGC
FokI 857 CATCC GGATG(9/13)
804 GGATG
ItaI 199 GCAGC GC^[ACGT]GC
960 GCCGC
Ksp632I 838 CTCTTC CTCTTC(1/4)
760 GAAGAG
MfeI 444 CAATTG C^AATTG
1625 CAATTG
MslI 184 CATCGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
795 CATAATCATG
MunI 444 CAATTG C^AATTG
1625 CAATTG
NciI 1232 CCCGG CC^[GC]GG
1233 CCGGG
NlaIV 481 GGCTCC GG[ACGT]^[ACGT]CC
1228 GGATCC
NsiI 503 ATGCAT ATGCA^T
1171 ATGCAT
PleI 836 GACTC GAGTC(4/5)
1078 GAGTC
PshBI 433 ATTAAT AT^TAAT
1488 ATTAAT
SfcI 112 CTACAG C^T[GA][CT]AG
580 CTACAG
SspBI 516 TGTACA T^GTACA
1513 TGTACA
StyI 287 CCAAGG C^C[AT][AT]GG
1801 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BclI BfaI BsaJI BsmAI BsrI
BssKI BstNI BstYI Cac8I DdeI
DpnII EcoRII FbaI HinfI HphI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NspI RsaI Sau3AI ScrFI SfaNI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoII