(Circular) (Six-base) MAPSORT of: pFN21AE4164.seq Check: 1381 from: 1 to: 6529 pFN21AE4164 6529 bp With 182 enzymes: SgfI * March 28, 2012 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3614 3614 Size: 6529 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4339 278 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3705 3872 1937 Size: 1768 167 4594 Fragments arranged by size: 4594 1768 167 Acc65I G'GTAC_C Cuts at: 3850 3850 Size: 6529 AclI AA'CG_TT Cuts at: 1563 4656 5029 1563 Size: 3093 373 3063 Fragments arranged by size: 3093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2643 2729 4592 5976 6244 1632 Size: 1011 86 1863 1384 268 1917 Fragments arranged by size: 1917 1863 1384 1011 268 86 AflII C'TTAA_G Cuts at: 820 1017 2107 820 Size: 197 1090 5242 Fragments arranged by size: 5242 1090 197 AflIII A'CryG_T Cuts at: 1203 2039 5428 6405 1203 Size: 836 3389 977 1327 Fragments arranged by size: 3389 1327 977 836 AgeI A'CCGG_T Cuts at: 5360 5360 Size: 6529 AhdI GACnn_n'nnGTC Cuts at: 5258 5258 Size: 6529 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3361 1184 Size: 2177 4352 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 2741 Size: 32 6497 AlwNI CAG_nnn'CTG Cuts at: 4272 5844 4272 Size: 1572 4957 ApaLI G'TGCA_C Cuts at: 4586 5742 4586 Size: 1156 5373 ApoI r'AATT_y Cuts at: 1493 2049 2122 2572 3648 3764 3838 4064 4216 Size: 556 73 450 1076 116 74 226 152 Cuts at: 4216 6325 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1076 556 450 226 152 116 74 73 AseI AT'TA_AT Cuts at: 160 1032 2439 3494 5083 160 Size: 872 1407 1055 1589 1606 Fragments arranged by size: 1606 1589 1407 1055 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 AvaI C'yCGr_G Cuts at: 1870 1943 3237 3854 1870 Size: 73 1294 617 4545 Fragments arranged by size: 4545 1294 617 73 BamHI G'GATC_C Cuts at: 3233 3859 4226 3233 Size: 626 367 5536 Fragments arranged by size: 5536 626 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3850 5305 618 Size: 325 217 627 2063 1455 1842 Fragments arranged by size: 2063 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3848 1391 Size: 2457 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5558 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4720 4754 1903 Size: 34 2783 34 3678 Fragments arranged by size: 3678 2783 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4421 5631 4421 Size: 1210 5319 BclI T'GATC_A Cuts at: 1548 2503 3103 3442 1548 Size: 955 600 339 4635 Fragments arranged by size: 4635 955 600 339 BfrBI ATG'CAT Cuts at: 2510 3178 2510 Size: 668 5861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5140 136 Size: 107 122 71 631 605 3468 1525 Fragments arranged by size: 3468 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6524 6524 Size: 6529 BlpI GC'TnA_GC Cuts at: 3946 3946 Size: 6529 Bme1580I G_kGCm'C Cuts at: 1790 4590 5746 1790 Size: 2800 1156 2573 Fragments arranged by size: 2800 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4260 5218 442 Size: 416 53 83 399 24 2843 958 1753 Fragments arranged by size: 2843 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5189 1239 Size: 3950 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4654 5519 5817 4654 Size: 865 298 5366 Fragments arranged by size: 5366 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3795 5192 882 Size: 617 2296 1397 2219 Fragments arranged by size: 2296 2219 1397 617 BsaAI yAC'GTr Cuts at: 493 1204 4320 493 Size: 711 3116 2702 Fragments arranged by size: 3116 2702 711 BsaBI GATnn'nnATC Cuts at: 6523 6523 Size: 6529 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4336 4718 5353 275 Size: 53 83 186 936 2803 382 635 1451 Fragments arranged by size: 2803 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4962 5360 5634 5781 6192 4962 Size: 398 274 147 411 5299 Fragments arranged by size: 5299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6304 Fragments arranged by size: 6304 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2750 1186 Size: 1564 4965 BseYI C'CCAG_C Cuts at: 5732 6228 5732 Size: 496 6033 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3630 3719 1163 Size: 573 1894 89 3973 Fragments arranged by size: 3973 1894 573 89 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3979 4740 5768 1540 Size: 275 186 1978 761 1028 2301 Fragments arranged by size: 2301 1978 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4590 4675 5746 4590 Size: 85 1071 5373 Fragments arranged by size: 5373 1071 85 BsmI GAATG_Cn' Cuts at: 4040 4133 4040 Size: 93 6436 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3848 4590 4675 5746 1391 Size: 399 2058 742 85 1071 2174 Fragments arranged by size: 2174 2058 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6192 6192 Size: 6529 BspHI T'CATG_A Cuts at: 3091 4418 6148 3091 Size: 1327 1730 3472 Fragments arranged by size: 3472 1730 1327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3614 3691 3884 844 Size: 2770 77 193 3489 Fragments arranged by size: 3489 2770 193 77 BsrBI CCG'CTC Cuts at: 1394 1823 4416 1394 Size: 429 2593 3507 Fragments arranged by size: 3507 2593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5024 5198 65 Size: 1183 3776 174 1396 Fragments arranged by size: 3776 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5173 5360 1700 Size: 110 3363 187 2869 Fragments arranged by size: 3363 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2521 3518 96 Size: 2425 997 3107 Fragments arranged by size: 3107 2425 997 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6529 BssSI C'ACGA_G Cuts at: 4589 5601 4589 Size: 1012 5517 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6529 BstBI TT'CG_AA Cuts at: 5333 5333 Size: 6529 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6529 BstYI r'GATC_y Cuts at: 1972 2993 3233 3596 3859 4226 4611 4628 6080 Size: 1021 240 363 263 367 385 17 1452 Cuts at: 6080 6166 6178 6524 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1021 385 367 363 346 263 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3806 513 Size: 552 939 1802 3236 Fragments arranged by size: 3236 1802 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5790 Fragments arranged by size: 5790 683 56 BtsI GCAGTG_nn' Cuts at: 2218 2422 4045 4839 4859 2218 Size: 204 1623 794 20 3888 Fragments arranged by size: 3888 1623 794 204 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6529 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6494 DraI TTT'AAA Cuts at: 2548 3424 3833 2548 Size: 876 409 5244 Fragments arranged by size: 5244 876 409 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5536 809 Size: 507 4220 1802 Fragments arranged by size: 4220 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3976 4865 6494 Size: 54 1425 50 275 18 2146 889 1629 Cuts at: 6494 8 Size: 43 Fragments arranged by size: 2146 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3976 1537 Size: 275 2164 4090 Fragments arranged by size: 4090 2164 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2760 2849 4459 1353 Size: 1407 89 1610 3423 Fragments arranged by size: 3423 1610 1407 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5116 5490 5636 387 Size: 1064 138 3527 374 146 1280 Fragments arranged by size: 3527 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2643 2729 4592 5189 5976 6244 1239 Size: 393 1011 86 1863 597 787 268 1524 Fragments arranged by size: 1863 1524 1011 787 597 393 268 86 EcoRI G'AATT_C Cuts at: 2049 3838 4216 2049 Size: 1789 378 4362 Fragments arranged by size: 4362 1789 378 EcoRV GAT'ATC Cuts at: 3297 3297 Size: 6529 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2666 2666 Size: 6529 FspI TGC'GCA Cuts at: 5035 5035 Size: 6529 HaeII r_GCGC'y Cuts at: 1678 5676 1678 Size: 3998 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2088 2120 2707 2739 3195 Size: 32 135 32 516 32 587 32 456 Cuts at: 3195 3227 3251 3283 3434 3466 5170 5202 5244 Size: 32 24 32 151 32 1704 32 42 Cuts at: 5244 5276 1373 Size: 32 2626 Fragments arranged by size: 2626 1704 587 516 456 151 135 42 32 32 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 3873 4119 669 Size: 988 281 1935 246 3079 Fragments arranged by size: 3079 1935 988 281 246 HindIII A'AGCT_T Cuts at: 748 2409 748 Size: 1661 4868 HpaI GTT'AAC Cuts at: 4119 4119 Size: 6529 KpnI G_GTAC'C Cuts at: 3854 3854 Size: 6529 MfeI C'AATT_G Cuts at: 2449 3630 4128 2449 Size: 1181 498 4850 Fragments arranged by size: 4850 1181 498 MluI A'CGCG_T Cuts at: 6405 6405 Size: 6529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2636 5643 5827 1305 Size: 1331 3007 184 2007 Fragments arranged by size: 3007 2007 1331 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4707 Fragments arranged by size: 4707 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2193 2804 4487 4846 5005 518 Size: 648 1027 611 1683 359 159 2042 Fragments arranged by size: 2042 1683 1027 648 611 359 159 MspA1I CmG'CkG Cuts at: 1606 3945 4622 5770 6015 1606 Size: 2339 677 1148 245 2120 Fragments arranged by size: 2339 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3806 513 Size: 552 939 1802 3236 Fragments arranged by size: 3236 1802 939 552 NdeI CA'TA_TG Cuts at: 387 2017 387 Size: 1630 4899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6529 NotI GC'GGCC_GC Cuts at: 3976 3976 Size: 6529 NsiI A_TGCA'T Cuts at: 2512 3180 2512 Size: 668 5861 NspI r_CATG'y Cuts at: 1132 1229 2043 2211 3348 3887 5432 1132 Size: 97 814 168 1137 539 1545 2229 Fragments arranged by size: 2229 1545 1137 814 539 168 97 PacI TTA_AT'TAA Cuts at: 3498 3498 Size: 6529 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6529 PciI A'CATG_T Cuts at: 2039 5428 2039 Size: 3389 3140 PflMI CCAn_nnn'nTGG Cuts at: 1108 3291 1108 Size: 2183 4346 PmeI GTTT'AAAC Cuts at: 3833 3833 Size: 6529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4598 4630 6111 6143 4598 Size: 32 1481 32 4984 Fragments arranged by size: 4984 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6529 PsiI TTA'TAA Cuts at: 4099 4099 Size: 6529 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3163 3195 3348 3380 3163 Size: 32 153 32 6312 Fragments arranged by size: 6312 153 32 32 PstI C_TGCA'G Cuts at: 830 3881 830 Size: 3051 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6529 SalI G'TCGA_C Cuts at: 1936 3871 1936 Size: 1935 4594 SbfI CC_TGCA'GG Cuts at: 3881 3881 Size: 6529 ScaI AGT'ACT Cuts at: 2583 4777 6489 2583 Size: 2194 1712 2623 Fragments arranged by size: 2623 2194 1712 SexAI A'CCwGG_T Cuts at: 3110 3110 Size: 6529 SfcI C'TryA_G Cuts at: 826 1045 2117 2585 3877 5012 5693 5884 826 Size: 219 1072 468 1292 1135 681 191 1471 Fragments arranged by size: 1471 1292 1135 1072 681 468 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6529 SmaI CCC'GGG Cuts at: 1872 3239 3856 1872 Size: 1367 617 4545 Fragments arranged by size: 4545 1367 617 SmlI C'TyrA_G Cuts at: 820 1017 1943 2107 4633 5534 5796 820 Size: 197 926 164 2526 901 262 1553 Fragments arranged by size: 2526 1553 926 901 262 197 164 SnaBI TAC'GTA Cuts at: 493 4320 493 Size: 3827 2702 SpeI A'CTAG_T Cuts at: 152 3457 152 Size: 3305 3224 SphI G_CATG'C Cuts at: 1132 2211 3887 1132 Size: 1079 1676 3774 Fragments arranged by size: 3774 1676 1079 SspI AAT'ATT Cuts at: 5 52 3024 4453 5 Size: 47 2972 1429 2081 Fragments arranged by size: 2972 2081 1429 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6529 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 3806 3968 513 Size: 552 939 288 1514 162 3074 Fragments arranged by size: 3074 1514 939 552 288 162 SwaI ATTT'AAAT Cuts at: 2548 2548 Size: 6529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4556 4573 4726 4911 5356 325 Size: 1255 2976 17 153 185 445 1498 Fragments arranged by size: 2976 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2113 2521 2581 Size: 274 80 33 51 1443 136 408 60 Cuts at: 2581 3518 4775 6487 96 Size: 937 1257 1712 138 Fragments arranged by size: 1712 1443 1257 937 408 274 138 136 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6489 6521 6489 Size: 32 6497 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6529 XbaI T'CTAG_A Cuts at: 3865 3865 Size: 6529 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6529 XmaI C'CCGG_G Cuts at: 1870 3237 3854 1870 Size: 1367 617 4545 Fragments arranged by size: 4545 1367 617 XmnI GAAnn'nnTTC Cuts at: 3023 4658 3023 Size: 1635 4894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4337 276 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI XcmI