Restriction map for
pFN21AE0774
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstNI BstPI
BstUI Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvaI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 1194 GTAC GT^AC
AlwNI 1016 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1083 GGGCCC GGGCC^C
Asp700I 1099 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 717 GTGCTC G[AT]GC[AT]^C
BalI 1285 TGGCCA TGG^CCA
BanI 40 GGCACC G^G[CT][GA]CC
BbsI 213 GTCTTC GAAGAC(2/6)
BclI 1110 TGATCA T^GATCA
BcnI 55 CCCGG CC^[GC]GG
BpmI 1119 CTCCAG CTGGAG(16/14)
BpuAI 213 GTCTTC GAAGAC(2/6)
BsaAI 1195 TACGTA [CT]AC^GT[GA]
BsaI 58 GGTCTC GGTCTC(1/5)
BsiHKAI 717 GTGCTC G[AT]GC[AT]^C
BspHI 1037 TCATGA T^CATGA
BssKI 55 CCCGG ^CC[ACGT]GG
Bst1107I 740 GTATAC GTA^TAC
BstF5I 702 GGATG GGATG(2/0)
BstZ17I 740 GTATAC GTA^TAC
EaeI 1285 TGGCCA [CT]^GGCC[GA]
EcoT22I 449 ATGCAT ATGCA^T
FauI 222 GCGGG CCCGC(4/6)
FbaI 1110 TGATCA T^GATCA
FokI 702 GGATG GGATG(9/13)
HapII 56 CCGG C^CGG
HgaI 87 GACGC GACGC(5/10)
HindIII 782 AAGCTT A^AGCTT
HpaII 56 CCGG C^CGG
MaeII 1196 ACGT A^CGT
MluNI 1285 TGGCCA TGG^CCA
MscI 1285 TGGCCA TGG^CCA
MslI 1150 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 81 CCGCTG C[AC]G^C[GT]G
MspI 56 CCGG C^CGG
NciI 55 CCCGG CC^[GC]GG
NsiI 449 ATGCAT ATGCA^T
NspI 681 ACATGC [GA]CATG^[CT]
PacI 658 TTAATTAA TTAAT^TAA
RcaI 1037 TCATGA T^CATGA
RsaI 1194 GTAC GT^AC
ScrFI 55 CCCGG CC^[ACGT]GG
SnaBI 1195 TACGTA TAC^GTA
TaqI 422 TCGA T^CGA
Tsp45I 296 GTGAC ^GT[GC]AC
TthHB8I 422 TCGA T^CGA
XmnI 1099 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 740 GTATAC GT^[AC][GT]AC
1144 GTCTAC
AciI 81 CCGC CCGC(-3/-1)
222 GCGG
AseI 453 ATTAAT AT^TAAT
661 ATTAAT
AsnI 453 ATTAAT AT^TAAT
661 ATTAAT
BanII 1083 GGGCCC G[GA]GC[CT]^C
1116 GGGCTC
BfaI 359 CTAG C^TAG
386 CTAG
BsmAI 853 GAGAC GTCTC(1/5)
59 GTCTC
BsmFI 678 GGGAC GGGAC(10/14)
1204 GGGAC
BsrI 510 CCAGT ACTGG(1/-1)
1121 CCAGT
BstXI 878 CCAAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1149 CCACTACAATGG
DdeI 347 CTGAG C^T[ACGT]AG
720 CTCAG
EcoT14I 413 CCTTGG C^C[AT][AT]GG
878 CCAAGG
MaeI 359 CTAG C^TAG
386 CTAG
MaeIII 296 GTGAC ^GT[ACGT]AC
1299 GTTAC
PshBI 453 ATTAAT AT^TAAT
661 ATTAAT
PstI 377 CTGCAG CTGCA^G
1208 CTGCAG
SapI 238 GAAGAGC GCTCTTC(1/4)
424 GAAGAGC
SfaNI 496 GATGC GCATC(5/9)
607 GATGC
StyI 413 CCTTGG C^C[AT][AT]GG
878 CCAAGG
TspRI 849 CAGTG CA[GC]TG[ACGT][ACGT]^
1122 CAGTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BstYI Cfr13I DpnII EarI
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MboI MboII MflI MnlI
MseI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I SfcI TfiI
Tru9I TseI Tsp509I XhoII